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Journals / NanoEra / 2025 / Volume: 5 Issue: 2

An investigation on the synthesis, characterisation and antibacterial activity of silver nanoparticles (Ag-NPs)-modified montmorillonite

Sümeyra Zahide Öztürk(İSTANBUL GELİŞİM ÜNİVERSİTESİ, GELİŞİM MESLEK YÜKSEKOKULU)
Alican Bahadır Semerci(NECMETTİN ERBAKAN ÜNİVERSİTESİ)0000-0001-9502-9321
Sıla Topçu(SAKARYA UYGULAMALI BİLİMLER ÜNİVERSİTESİ)0000-0003-0681-8033
Murat Tuna(SAKARYA ÜNİVERSİTESİ)0000-0002-8554-903X
Fatih Çalışkan(SAKARYA UYGULAMALI BİLİMLER ÜNİVERSİTESİ, TEKNOLOJİ FAKÜLTESİ)0000-0002-9568-7049
Journal
NanoEra
Pages
—
Publication Date
2025-12-31
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings highlight Ag-MMT as a promising nanocomposite for antimicrobial applications.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, silver nanoparticle (Ag-NP) modified sodium montmorillonite (Na-MMT) was synthesised via a sol–gel-assisted ion-exchange approach.
📊
Key Findings & Data:The antibacterial performance of six Ag-MMT formulations was assessed via the Kirby–Bauer disk diffusion assay against Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Salmonella typhimurium, Staphylococcus epidermidis, and Staphylococcus aureus.
💡
Conclusion & Impact:These findings highlight Ag-MMT as a promising nanocomposite for antimicrobial applications.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Siver · Cation Exchange · Organoclay

Abstract

In this study, silver nanoparticle (Ag-NP) modified sodium montmorillonite (Na-MMT) was synthesised via a sol–gel-assisted ion-exchange approach. During the process, Ag⁺ cations were intercalated into the interlayer spaces of Na-MMT, leading to the successful preparation of Ag-enriched organoclays. Structural modifications were verified by Fourier-transform infrared spectroscopy (FTIR), while scanning electron microscopy (SEM) revealed well-dispersed silver nanoparticles on the clay surface. Energy-dispersive X-ray spectroscopy (EDS) provided additional confirmation of silver incorporation within the layered framework. The antibacterial performance of six Ag-MMT formulations was assessed via the Kirby–Bauer disk diffusion assay against Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Salmonella typhimurium, Staphylococcus epidermidis, and Staphylococcus aureus. The most effective sample exhibited a maximum inhibition zone of 13.5 mm against S. typhimurium, while a 2% Ag loading was found to be the minimum effective concentration for broad-spectrum antibacterial activity. These findings highlight Ag-MMT as a promising nanocomposite for antimicrobial applications.

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Cite This Article

Öztürk, S. Z., Semerci, A. B., Topçu, S., Tuna, M., Çalışkan, F. (2025). An investigation on the synthesis, characterisation and antibacterial activity of silver nanoparticles (Ag-NPs)-modified montmorillonite. NanoEra, Volume: 5 Issue: 2. https://ajindex.com/articles/1627649

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References (38)

  1. Chimeddorj M. Farklı Bentonitlerin Nem Alıcı (Desikant) Özelliklerinin Belirlenmesi. İstanbul Teknik Üniversitesi; 2007.
  2. Edraki M, Moghaddampour IM, Alinia-Ahandani E, Keivani MB, Sheydaei M. Ginger intercalated sodium montmorillonite nano clay: assembly, characterization, and investigation antimicrobial properties. Chem Rev Lett. 2021;4:120-129. doi:https://doi.org/10.22034/crl.2021.273340.1103
  3. Peighambardoust SJ, Zahed-Karkaj S, Peighambardoust SH, Ebrahimi Y, Peressini D. Characterization of carboxymethyl cellulose-based active films incorporating non-modified and Ag or Cu-modified Cloisite 30B and montmorillonite nanoclays. Iran Polym J. 2020;29(12):1087-1097. doi:10.1007/s13726-020-00863-z
  4. Chinoune K, Bentaleb K, Bouberka Z, Nadim A, Maschke U. Adsorption of reactive dyes from aqueous solution by dirty bentonite. Appl Clay Sci. 2016;123:64-75. doi:10.1016/j.clay.2016.01.006
  5. El-Enein SA, Okbah MA, Hussain SG, Soliman NF, Ghounam HH. Adsorption of Selected Metals Ions in Solution Using Nano-Bentonite Particles: Isotherms and Kinetics. Environ Process. 2020;7(2):463-477. doi:10.1007/s40710-020-00430-x
  6. Gu G, Hu J, Cevallos-Cevallos JM, Richardson SM, Bartz JA, van Bruggen AHC. Internal Colonization of Salmonella enterica Serovar Typhimurium in Tomato Plants. Ravel J, ed. PLoS One. 2011;6(11):e27340. doi:10.1371/journal.pone.0027340
  7. Tohdee K, Kaewsichan L, Asadullah. Enhancement of adsorption efficiency of heavy metal Cu(II) and Zn(II) onto cationic surfactant modified bentonite. J Environ Chem Eng. 2018;6(2):2821-2828. doi:10.1016/j.jece.2018.04.030
  8. Shameli K, Mansor Bin Ahmad M, Mohsen Z, Yunis WZ, Ibrahim NA, Rustaiyan A. Synthesis of silver nanoparticles in montmorillonite and their antibacterial behavior. Int J Nanomedicine. 2011;6:581-590. doi:10.2147/IJN.S17112
  9. Teich-McGoldrick SL, Greathouse JA, Jové-Colón CF, Cygan RT. Swelling Properties of Montmorillonite and Beidellite Clay Minerals from Molecular Simulation: Comparison of Temperature, Interlayer Cation, and Charge Location Effects. J Phys Chem C. 2015;119(36):20880-20891. doi:10.1021/acs.jpcc.5b03253
  10. Lin T, Wang J, Yin X, Wei X. Modification of Bentonite and Its Application in Antimicrobial Material. Pap Biomater. 2020;5(1):54-61. doi:https://doi.org/10.12103/j.issn.2096-2355.2020.01.006
  11. Chen WL, Grabowski RC, Goel S. Clay Swelling: Role of Cations in Stabilizing/Destabilizing Mechanisms. ACS Omega. 2022;7(4):3185-3191. doi:10.1021/acsomega.1c04384
  12. Çankaya N, Sökmen Ö. Biyopolimerler ve Montmorillonit Kil Nanokompozitleri. J Polytech. 2017;20(3):663-673. 13. Jiao L, Lin F, Cao S, et al. Preparation, characterization, antimicrobial and cytotoxicity studies of copper/zincloaded montmorillonite. J Anim Sci Biotechnol. 2017;8(1):27. doi:10.1186/s40104-017-0156-6
  13. Tan S, Zhang K, Zhang L, Xie Y, Liu Y. Preparation and Characterization of the Antibacterial Zn 2+ or/and Ce 3+ Loaded Montmorillonites. Chinese J Chem. 2008;26(5):865-869. doi:10.1002/cjoc.200890160
  14. Kaviyarasu K, Geetha N, Kanimozhi K, et al. In vitro cytotoxicity effect and antibacterial performance of human lung epithelial cells A549 activity of Zinc oxide doped TiO2 nanocrystals: Investigation of bio-medical application by chemical method. Mater Sci Eng C. 2017;74:325-333. doi:10.1016/j.msec.2016.12.024
  15. Mobeen Amanulla A, Jasmine Shahina S, Sundaram R, et al. Antibacterial, magnetic, optical and humidity sensor studies of β-CoMoO4 - Co3O4 nanocomposites and its synthesis and characterization. J Photochem Photobiol B Biol. 2018;183:233-241. doi:10.1016/j.jphotobiol.2018.04.034
  16. Li M, Huang L, Wang X, et al. Direct generation of Ag nanoclusters on reduced graphene oxide nanosheets for efficient catalysis, antibacteria and photothermal anticancer applications. J Colloid Interface Sci. 2018;529:444-451. doi:10.1016/j.jcis.2018.06.028
  17. Magaña SM, Quintana P, Aguilar DH, et al. Antibacterial activity of montmorillonites modified with silver. J Mol Catal A Chem. 2008;281(1-2):192-199. doi:10.1016/j.molcata.2007.10.024
  18. Sharma A, Saini AK, Kumar N, et al. Methods of preparation of metal-doped and hybrid tungsten oxide nanoparticles for anticancer, antibacterial, and biosensing applications. Surfaces and Interfaces. 2022;28:101641. doi:10.1016/j.surfin.2021.101641
  19. Kiradzhiyska D, Milcheva N, Mancheva R, Batsalova T, Dzhambazov B, Zahariev N. Preparation and Preliminary Evaluation of Silver-Modified Anodic Alumina for Biomedical Applications. Metals (Basel). 2021;12(1):51. doi:10.3390/met12010051
  20. Mofa NN, Zhapekova AO, Sadykov BS, Bakkara AE, Tulepov MI, Elouadi B. Preparation of Silvered Colloidal Compositions for Nanocosmetic Drugs. Eurasian Chem J. 2020;22(1):11-18. doi:10.18321/ectj925
  21. Subha V, Ranu A, Shankar A, et al. Functionalization ofspraycoated cellulose nanofiber sheet with montmorillonite (MMT) and silver nanoparticles (AgNPs) to biomedical nanocomposite as wound regeneration scaffold. Prog Org Coatings. 2022;166:106782. doi:10.1016/j.porgcoat.2022.106782
  22. Unsal T, Cansever N, Ilhan-Sungur E. The influence of Ag-Cu ions on natural biofilms of variable ages: Evaluation of MIC. Bioelectrochemistry. 2022;146:108143. doi:10.1016/j.bioelechem.2022.108143
  23. Abdelkrim S, Mokhtar A, Djelad A, et al. Chitosan/AgBentonite Nanocomposites: Preparation, Characterization, Swelling and Biological Properties. J Inorg Organomet Polym Mater. 2020;30(3):831-840. doi:10.1007/s10904-019-01219-8
  24. Lin J, Jiang B, Zhan Y. Effect of pre-treatment of bentonite with sodium and calcium ions on phosphate adsorption onto zirconium-modified bentonite. J Environ Manage. 2018;217:183-195. doi:10.1016/j.jenvman.2018.03.079
  25. Soy U, Demir A, Caliskan F. Effect of bentonite addition on fabrication of reticulated porous SiC ceramics for liquid metal infiltration. Ceram Int. 2011;37(1):15-19. doi:10.1016/j.ceramint.2010.07.028
  26. Bai X, Sandukas S, Appleford M, Ong JL, Rabiei A. Antibacterial effect and cytotoxicity of Ag‐doped functionally graded hydroxyapatite coatings. J Biomed Mater Res Part B Appl Biomater. 2012;100B(2):553-561. doi:10.1002/jbm.b.31985
  27. Huang X, Ge M, Wang H, Liang H, Meng N, Zhou N. Functional modification of polydimethylsiloxane nanocomposite with silver nanoparticles-based montmorillonite for antibacterial applications. Colloids Surfaces A Physicochem Eng Asp. 2022;642:128666. doi:10.1016/j.colsurfa.2022.128666
  28. Sohrabnezhad S, Rassa M, Seifi A. Green synthesis of Ag nanoparticles in montmorillonite. Mater Lett. 2016;168:28-30. doi:10.1016/j.matlet.2016.01.025
  29. Zhou C, Tong D, Yu W. Smectite Nanomaterials: Preparation, Properties, and Functional Applications. In: Nanomaterials from Clay Minerals. Elsevier; 2019:335-364. doi:10.1016/B978-0-12-814533-3.00007-7
  30. Roy A, Butola BS, Joshi M. Synthesis, characterization and antibacterial properties of novel nano-silver loaded acid activated montmorillonite. Appl Clay Sci. 2017;146:278-285. doi:10.1016/j.clay.2017.05.043
  31. Baran A. Gümüş nano malzemelerin çevre dostu, hızlı sentezi ve biomedikal uygulamaları. DÜMF Mühendislik Derg. 2021;12(2):329-336. doi:10.24012/dumf.880878
  32. Taaca KLM, Dahonog LA, Olegario EM. Cell viability and bacterial reduction activity of Ag-modified bentonite. Mater Today Proc. 2019;16:1782-1788. doi:10.1016/j.matpr.2019.06.051
  33. Krishnan B, Mahalingam S. Facile synthesis and antimicrobial activity of manganese oxide/bentonite nanocomposites. Res Chem Intermed. 2017;43(4):2351-2365. doi:10.1007/s11164-016-2765-7
  34. Banerjee M, Mallick S, Paul A, Chattopadhyay A, Ghosh SS. Heightened Reactive Oxygen Species Generation in the Antimicrobial Activity of a Three Component IodinatedChitosan−Silver Nanoparticle Composite. Langmuir. 2010;26(8):5901-5908. doi:10.1021/la9038528
  35. Cui L, Chen P, Chen S, et al. In Situ Study of the Antibacterial Activity and Mechanism of Action of Silver Nanoparticles by Surface-Enhanced Raman Spectroscopy. Anal Chem. 2013;85(11):5436-5443. doi:10.1021/ac400245j
  36. Joe A, Park SH, Kim DJ, et al. Antimicrobial activity of ZnO nanoplates and its Ag nanocomposites: Insight into an ROSmediated antibacterial mechanism under UV light. J Solid State Chem. 2018;267:124-133. doi:10.1016/j.jssc.2018.08.003
  37. Vasiliev G, Kubo AL, Vija H, et al. Synergistic antibacterial effect of copper and silver nanoparticles and their mechanism of action. Sci Rep. 2023;13(1):9202. doi:10.1038/s41598-023-36460-2
  38. Kukushkina EA, Hossain SI, Sportelli MC, Ditaranto N, Picca RA, Cioffi N. Ag-Based Synergistic Antimicrobial Composites. A Critical Review. Nanomaterials. 2021;11(7):1687. doi:10.3390/nano11071687