Journals / Astım Allerji İmmünoloji / 2011 / Cilt: 9 - Sayı: 1

Comparison between pollen and seed protein profiles of Corylus avellana

Corylus avellana polen ve tohum protein profillerinin karşılaştırılması

Pages
37–43
DOI
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AI Insight
⚡ TL;DR(Too Long; Didn't Read)

After the SDSPAGE analysis process, 22% of bands have disappeared in roasted seed and 9 and 17 kDa bands were heat resistant while 14 kDa was heat sensitive.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: It is known that pollens from trees of the order Fagales usually cause allergic reactions.
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Key Findings & Data:Hazelnut seeds which are consumed as roasted have a commercial prominence.Conclusion: As a result, we thought that hazel allergy tests obtained from the regional resources may contribute the treatment of food allergies. (Asthma Allergy Immunol 2011;9:37-43) Materials and Methods: In this study, protein profiles of hazelnut seeds and pollens have been taken out with SDS-PAGE method and compared with each other.
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Conclusion & Impact:After the SDSPAGE analysis process, 22% of bands have disappeared in roasted seed and 9 and 17 kDa bands were heat resistant while 14 kDa was heat sensitive.
🔬Design:Research Article
📍Scope:2011 Verileri
⚙️Variables:Domain Parameters

Abstract

Objective: It is known that pollens from trees of the order Fagales usually cause allergic reactions. It is also known that cross reactivity takes part as a trigger in the development of allergies. Cross reactivity may occur between different allergen sources. Moreover, it can be found between different biological structures of same source such as pollen and seed. Pollen and seed of hazelnut can cause allergic reactions. Hazelnut seeds which are consumed as roasted have a commercial prominence.Conclusion: As a result, we thought that hazel allergy tests obtained from the regional resources may contribute the treatment of food allergies. (Asthma Allergy Immunol 2011;9:37-43) Materials and Methods: In this study, protein profiles of hazelnut seeds and pollens have been taken out with SDS-PAGE method and compared with each other. In addition, effects of heating process on seed protein profile have been investigated. Results: When the results have taken, it is seen that 8 same bands were found between pollen and seed at the end of SDS-PAGE analysis. After the SDSPAGE analysis process, 22% of bands have disappeared in roasted seed and 9 and 17 kDa bands were heat resistant while 14 kDa was heat sensitive.

Özet

Giriş: Genellikle Fagales ordosuna dahil ağaçların polenlerinin birçok allerjik reaksiyona yol açtığı bilinmektedir. Bununla birlikte allerjinin gelişiminde çapraz reaktivite kaynaklı olguların rol oynadığı görülmektedir. Çapraz reaktivite, farklı allerjen kaynakları arasında görülebildiği gibi, polen ve tohum gibi aynı kaynağın farklı biyolojik yapıları arasında da görülebilmektedir. Fındık bitkisi polenleri ve tohumları allerjik reaksiyonlara neden olabilmektedir. Aynı zamanda kavrularak tüketilen fındık tohumları ticari öneme sahiptir. Gereç ve Yöntem: Bu çalışmada, fındık bitkisi tohumları ile polenlerinin protein profilleri, SDS-PAGE yöntemiyle çıkartılıp karşılaştırılmıştır. Ayrıca, ısıl işlemlerin tohum protein profili üzerine olan etkileri de araştırılmıştır. Bulgular: Polen ve tohum örneklerinden elde edilen jel elektroforezi analizi sonuçlarında tohum ve polen arasında sekiz tane ortak bant bulunmuştur. Kavrulan fındık tohumunda, bantların yaklaşık %22’sinin kaybolduğu görülmüştür. Bununla birlikte, 9 ve 17 kDa molekül ağırlığına sahip bantların ısıya dayanıklı, 14 kDa’lık bantın ise ısıya dayanıksız olduğu bulunmuştur.Sonuç: Fındık allerji testlerinin bölgesel olarak elde edilen kaynaklar ile yapılmasının gıda allerjilerinin tedavisine katkıda bulunabileceğini düşünmekteyiz. (Asthma Allergy Immunol 2011;9:37-43)

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Cite This Article

CEBECİ, H., ALIÇ, G., YILDIRIM, Ö., PINAR, N. M., AŞÇI, B., ALAN, Ş. (2011). Comparison between pollen and seed protein profiles of Corylus avellana. Astım Allerji İmmünoloji, Cilt: 9 - Sayı: 1, 37–43. https://ajindex.com/articles/161914

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References (11)

  1. Gruehna S, Suphioglu C, O’Hehirc RE, Volkmann D. Molecular cloning and characterization of hazel pollen protein (70 kD) as a luminal binding protein (BiP): a novel cross-reactive plant allergen. Int Arch Allergy Immunol 2003;131:91-100.
  2. Kazemi-Shirazi L, Pauli G, Purohit A, Spitzauer S, Fröschl R, Hoffmann-Sommergruber K, et al. Quantitative IgE inhibition experiments with purified recombinant allergens indicate pollen-derived allergens as the sensitizing agents responsible for many forms of plant food allergy. J Allergy Clin Immunol 2000;105:116-25.
  3. Scheurer S, Son DY, Boehm M, Karamloo F, Franke S, Hoffmann A, et al. Cross-reactivity and epitope analysis of Pru a1, the major cherry allergen. Mol Immunol 1999;36:155-67.
  4. Valenta R, Kraft D. Type 1 allergic reactions toplant-derived food: a consequence of primarysensitization to pollen allergens. J Allergy Clin Immunol 1996;97:893-5.
  5. Radauer C, Breiteneder H. Pollen allergens are restricted to few protein families and show distinct patterns of species distribution. J Allergy Clin Immunol 2006;117:141-7.
  6. Rudeschko O, Fahlbusch B, Steurich F, Schlenvoigt G, Jäger L. Kiwi allergens and their cross-reactivity with birch, rye, timothy, and mugwort pollen. J Invest Allergol Clin Immunol 1998;8:78-84.
  7. Bauer L, Ebner C, Hirschwehr R, Wüthrich B, Pichler C, Fritsch R, et al. IgE cross-reactivity between birch pollen, mugwort pollen and celery is due to at least three distinct cross-reacting allergens: immunoblot investigation of the birch-mugwort-celery syndrome. Journal of the British Society for Allergy and Clinical Immunology 1996;26:1161-70.
  8. Schocker F, Lüttkopf D, Müller U, Thomas P, Vieths S, Becker WM. IgE binding to unique hazelnut allergens: Identification of non pollen-related and heat-stable hazelnut allergens eliciting severe allergic reactions. European Journal of Nutrition 2000;39:172-80.
  9. Hirschwehr R, Valenta R, Ebner C, Ferreira F, Sperr W R, Valent P, et al. Identification of common allergenic structures in hazel pollen and hazelnuts: a possible explanation for sensitivity to hazelnuts in patients allergic to tree pollen. J Allergy Clin Immunol 1992;90:927-36. Alan Ş. Zonguldak ili atmosferinin polen ve spor analizi (2003-2004) [Tez]. Zonguldak: Zonguldak Karaelmas Üniversitesi, 2004.
  10. İnceoğlu Ö, Pınar NM, Şakıyan N, Sorkun K. Airborne pollen concentration in Ankara, Turkey 1990-1993. Grana 1994;33:158-61.
  11. Ayvaz A, Baki A, Doğan C. Trabzon atmosferindeki aeroallerjenlerin mevsimsel dağılımı. Asthma