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Journals / Cukurova University Journal of Natural and Applied Sciences / 2025 / Volume: 4 Issue: 2

Acanthus spinosus L.: In Vitro Antimicrobial Activity, Fixed Oil Amount, Mineral Element Analysis, and Fatty Acid Composition (Kireç Plateau/ Kahramanmaraş)

Murat Gök(KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, BİYOLOJİ (YL) (TEZLİ))0009-0005-6214-0804
Sevil Toroglu(KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ, BİYOLOJİ PR.)0000-0001-8667-6383
Ferudun Koçer(KAHRAMANMARAŞ SÜTÇÜ İMAM ÜNİVERSİTESİ, ÜNİVERSİTE SANAYİ-KAMU İŞBİRLİĞİ GELİŞTİRME UYGULAMA VE ARAŞTIRMA MERKEZİ)0000-0002-8749-7106
Pages
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Publication Date
2025-12-29
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the ethyl acetate extract exhibited only very slight antifungal activity (7 mm inhibition zone) against Candida albicans ATCC 14053.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, samples of Acanthus spinosus L. were collected from Kireç Plateau (Kahramanmaraş) at 2024.
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Key Findings & Data:The antimicrobial activity of Acanthus spinosus L. was tested against 12 bacterial species and 1 fungal species using aqueous, methanol, ethanol, and ethyl acetate extracts by the disk diffusion method.
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Conclusion & Impact:The results showed that the ethyl acetate extract exhibited only very slight antifungal activity (7 mm inhibition zone) against Candida albicans ATCC 14053.
🔬Design:Research Article
📍Scope:2024 Verileri
⚙️Variables:Acanthus spinosus L. · Antimicrobial Activity · Fatty Acid Composition

Abstract

In this study, samples of Acanthus spinosus L. were collected from Kireç Plateau (Kahramanmaraş) at 2024. The purpose of the study was to research the antimicrobial activity, fixed oil content, mineral element combination, and fatty acid contents of Acanthus spinosus L. The antimicrobial activity of Acanthus spinosus L. was tested against 12 bacterial species and 1 fungal species using aqueous, methanol, ethanol, and ethyl acetate extracts by the disk diffusion method. The results showed that the ethyl acetate extract exhibited only very slight antifungal activity (7 mm inhibition zone) against Candida albicans ATCC 14053. The fixed oil amount of this plant was showed as 1.80% using the Randall extraction method. The fatty acid contents were analyzed by preparing methyl esters and using GC with a FID detector. The analysis identified 25 different phytochemical fatty acid components, of which 14 were unsaturated (38.452 %) and 11 were saturated fatty acids (61.549%). Capric acid had the highest proportion (31.290%) in the saturated fatty acids, while Oleic acid was the dominant in the unsaturated fatty acid (11.208%). For mineral element analysis, a microwave digestion system and an ICP-OES device were used. The above-ground parts of the plant were analyzed for mineral elements (Na, K, Ca, P, Mg, Fe, Mn, Zn, Cu, Mo). The findings indicate that Acanthus spinosus L. is a potential natural source with a rich fatty acid content and high calcium and zinc content, despite its very limited antifungal source.

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Cite This Article

Gök, M., Toroglu, S., Koçer, F. (2025). Acanthus spinosus L.: In Vitro Antimicrobial Activity, Fixed Oil Amount, Mineral Element Analysis, and Fatty Acid Composition (Kireç Plateau/ Kahramanmaraş). Cukurova University Journal of Natural and Applied Sciences, Volume: 4 Issue: 2. https://doi.org/10.70395/cunas.1787175

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References (35)

  1. Huang, M.Y., Zhong, L.J., Wang, F., Liu, Q.Y., Zhang, Y.H., (2014). Chemical constituents from the roots of Acanthus ilicifo-lius var. xiamenensis. Biochem. Systemat. Ecol. 55, 145–147.
  2. Matos, P., Paranhos, A., Oliveiros, B., Cruz, M. T., Batista, M. T., Figueirinha, A., (2024). Biological and phytochemical varia-tion with pre-and post-harvest conditions for the Acanthus mollis L. leaf. Industrial Crops and Products, 211, 118221.
  3. Fekih, N., Allali, H., Ziani-Cherif, C., Flamini, G., (2023). Acanthus mollis L. Grown in Algeria Exhibits Potent Antioxidant Activity. Agriculturae Conspectus Scientificus, 88(4), 301-306.
  4. TUBİVES., (2025). Türkiye Bitkileri Veri Servisi (Erişim tarihi: 20.07.2025) http://194.27.225.161/yasin/tubives/index.php?sayfa=1&tax_id=7386
  5. Korkut, T., (2012). The use of Acanthus in ancient art. In XI International Symposium on Flower Bulbs and Herbaceous Per-ennials 1002 (pp. 265-270).
  6. El Weshahy, M., Ellabban, E., (2022). Highlights on the Use of Acanthus as an Ornamental motif from Greco-Roman to Islam-ic period. Journal of Association of Arab Universities for Tourism and Hospitality.
  7. Özenver, N., Efferth, M., Efferth, T., (2022). Ethnopharmacology, phytochemistry, chemical ecology and invasion biology of Acanthus mollis L. Journal of Ethnopharmacology, 285, 114833.
  8. Carvalho, L., Barros, M., Fernandes, M., &Fernandes, F. M., (2025). A botanical perspective on the Portuguese crown jewels – the golden fleece insignia. Ethnobotany Research and Applications, 30, 1-13.
  9. Bulut, G., Haznedaroglu, M.Z., Doğan, A., Koyu, H., Tuzlacı, E., (2017). An ethnobotanical study of medicinal plants in Acipayam (Denizli- Türkiye). J. Herb. Med. 10, 64–81.
  10. Matos, P., Batista, M. T., Figueirinha, A., (2022). A review of the ethnomedicinal uses, chemistry, and pharmacological proper-ties of the genus Acanthus (Acanthaceae). Journal of Ethnopharmacology, 293, 115271.
  11. Aydın, S. S., (2019). Türkiye’de yetişen bazı Acanthus L. türlerinden ilaç adayı bileşiklerin izolasyonu. Sağlık Bilimleri Enstitüsü. Doktora tezi.
  12. Sarikurkcu, C., Kirkan, B., Sahinler, S. S., Tepe, B., (2022). Can Acanthus spinosus be used as an alternative antioxidant and enzyme inhibitory agent?. South AfricanJournal of Botany, 145, 135-141.A
  13. Büyüktuncel, E., (2012). Gelişmiş Ekstraksiyon Teknikleri I. (Advanced Extraction Techniques I) Hacettepe Üniversity, Journal of theFaculty of Pharmacy. Volume 32 / Issue 2. 209-242.
  14. Bauer, A.W., Kirby, W.M.M, Sherris, J.C, Turck, M., (1966). Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 36, 493-496.
  15. Keskin, D., Toroglu, S., (2011). Studies on antimicrobial activities of solvent extracts of different commercial spices. Journal of Environmental Biology, 32, 251-256.
  16. Alan, Ş., Koçer, F., Uyar, G., (2018). Antimicrobial activities of some bryophytes in Türkiye. Communications Faculty of Sci-ences University of Ankara Series C Biology, 27(2), 17-22.
  17. İmalı, A., Karakuzulu, H., Koçer, F., (2023). Determination of mineral content and antimicrobial activities of sumac (Rhuscoriar-ia L.) grown under Kilis ecological conditions (Türkiye). Open Access ResearchJournal of Life Sciences, 6(2), 25-33.
  18. Aşcı, Ö. A., Baydar, N. G., (2021). Exchange of total carbohydrate, minerals, and phenolics in grape and grape products. Turk-ish Journal of Agriculture-Food Science and Technology, 9(6), 1106-1113.
  19. Akıncı, A.B., Sünbül, A.B., Çaylar, M., Koçer, F., Uruş, S., (2022). “Aromatik Schiff Bazları ve Co(II), Ni(II) ve Cu(II) Kom-plekslerinin Sentezi: Antimikrobiyal Aktivitelerinin İncelenmesi”, El-Cezeri Journal of ScienceandEngineering, vol. 9, no. 3, pp. 1049–1070. doi: 10.31202/ecjse.109952.
  20. Başay, S., Dorak, S., Aşik, B. B., (2025). The Effects of Organic Fertilizer Applications on the Nutrient Elements Content of Eggplant Seeds. Agronomy, 15(2), 439.
  21. [Ashraf, M., Ali, Q., Iqbal, Z., (2006). Effect of nitrogen application rate on the content and composition of oil, essential oil and minerals in black cumin (Nigellasativa L.) seeds. J. Environ. Sci. Health B.86, 871-876.
  22. Hosseini, S.S., Rezadoost, H., Nadjafi, F., Asareh, M.H., (2019). Comparative essential oil composition and fatty acid profiling of some Iranian black cumin landraces. Ind. Crop. Prod. 140, 111628.
  23. Metcalfe, LD., Schmitz, A.A., Pelka, J.R., (1966). Rapid Preparation of Fatty Acid Esters from Lipids for Gas Chromatographic Analysis. Analytical Chemistry. 38/Issue 3, 514-515.
  24. Anonymous., (2011). Hayvansal ve bitkisel katı ve sıvı yağlar- yağ asitleri metil esterlerinin gaz kromatoğrafisi Bölüm 2: Yağ asitleri metil esterlerinin hazırlanması. TSE yayınları, TS No: 12966/2, Ankara.
  25. Eser, N., Yoldaş, A., Koçer, F., (2020). GC/FID ile ekstrakte edilen Ferula elaeochytris kök ekstresinden yağ asidlerinin analizi. Sakarya Tıp Dergisi, 10(2), 264-269.
  26. Bora, R., Adhikari, P.P., Das, A.K., Raaman, N., Sharma, G.D., (2017). Ethnomedicinal, phytochemical, and pharmacological aspects of genus Acanthus Int. J. Pharm. Pharmaceut. Sci., 9 (12), p. 18.
  27. Keskin, C., (2017). Investigations of in vitro antioxidant activities, elemental compositions and lipid constituents of Acanthus dioscoridis L. var. dioscoridis L. as a Medicinal Plant. Journal of the Institute of Scienceand Technology, 7(2), 141-148.
  28. Mu, Y.M., Yanase, T., Nishi, Y., Tanaka, A., Saito, M., Jin, C.H., Mukasa, C., Okabe, T., Nomura, M., Goto, K., Nawata, H., (2001). Saturated FFAs, palmitic acid and stearic acid, induce apoptosis in human granulosa cells. Endocrinology., 142: 3590-3597. doi:10.1210/endo.142.8.8293.
  29. Clifton, P., Dietary fatty acids and inflammation. Nutrition and Dietetics. (2009). 66(1):7–11. https://doi.org/10.1111/j.1747-0080.2008.01311.x
  30. Martin‐Moreno, J.M., Willett, W.C., Gorgojo, L., Banegas, J.R., Rodriguez‐Artalejo, F., Fernandez‐Rodriguez, J.C., Mai-sonneuve, P., Boyle, P., (1994). Dietary fat, olive oil intake and breast cancer risk. Int J Cancer. 58: 774-780. doi:10.1002/ijc.2910580604
  31. Coupland, K., Wilson, R,. (1994). Nervonic acid and demyelinating disease. Med Hypotheses. 42(4): 237-242.
  32. Dauqan, E., Sani, H.A., Abdullah, A., Kasim, ZM., (2011). Effect of Different Vegetable Oils (Red Palm Olein, Palm Olein, Corn Oil and Coconut Oil) on Lipid Profile in Rat. Food Nutr Sci.2,253-258.doi:10.4236/fns.2011.24036 Published Online June 2011 (http://www.scirp.org/journal/fns).
  33. Dugo, G., Pera, L., Torre, GL., Giuffrida, D., (2004). Determination of Cd(II), Cu(II), Pb(II), andZn(II) content in commercial vegetable oils using derivative potentiometric stripping analysis. Food Chemistry, 87, 639-645.
  34. Karaca, E., Aytaç, S., (2007). Yağ bitkilerinde yağ asitleri kompozisyonu üzerine etki eden faktörler. OMÜ Ziraat Fakültesi Dergisi, 22(1), 123-131.
  35. Can, N., (2019). The importance of oxidative stability in preservation of vegetable oils and analysis methods used to determine oxidative stability. ABMYO Dergisi Sayı 54, 107-124.