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Journals / Tıp Fakültesi Klinikleri Dergisi / 2026 / Cilt: 2 Sayı: 3

Effects of Short-term High Glucose on NIH/3T3 Fibroblast Proliferation, Apoptosis, and Collagen Type I Production

Kısa Süreli Yüksek Glikoz Uygulamasının NIH/3T3 Fibroblastlarında Proliferasyon, Apoptoz ve Kollajen Tip I Üretimi Üzerine Etkileri

Tuğba Soydaş(ISTANBUL AYDIN UNIVERSITY)
Elif Yaprak Saraç(ISTANBUL UNIVERSITY)
Suzan Çınar(ISTANBUL UNIVERSITY)
Güven Yenmiş(Acıbadem Healthcare Services)
Sibel Doğan(ISTANBUL UNIVERSITY)
Seyhun Solakoğlu(ISTANBUL UNIVERSITY)
Matem Tunçdemir(Istanbul University-Cerrahpasa)
Gönül Kanıgür Sultuybek(ISTANBUL AYDIN UNIVERSITY)
Pages
91–95
Publication Date
2019-08-09
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusions: High glucose modulates the fibrosis in NIH/3T3 fibroblast cells via inducing the production of type I collagen while maintains the homeostasis by inducing the apoptosis and necrosis of cells.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: To investigate the mechanisms of in vitro high glucose induced model of liver fibrosis.
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Key Findings & Data:Results: A high glucose medium not only increased NIH/3T3 fibroblast proliferation, but also increased type I collagen synthesis, showing a similar condition to the fibrosis.
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Conclusion & Impact:Conclusions: High glucose modulates the fibrosis in NIH/3T3 fibroblast cells via inducing the production of type I collagen while maintains the homeostasis by inducing the apoptosis and necrosis of cells.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:High glucose · Collagen · NIH/3T3 fibroblast

Abstract

Objective: To investigate the mechanisms of in vitro high glucose induced model of liver fibrosis. Material and methods: The effects of high glucose concentration on fibroblast proliferation were investigated by BrdU immunostaining. Apoptosis and necrosis levels were analyzed by flow cytometric assay. The content of collagen type I was measured by Collagen Estimation Assay through ELISA. Results: A high glucose medium not only increased NIH/3T3 fibroblast proliferation, but also increased type I collagen synthesis, showing a similar condition to the fibrosis. Moreover, the high glucose caused an increased level of cellular apoptosis and necrosis. Conclusions: High glucose modulates the fibrosis in NIH/3T3 fibroblast cells via inducing the production of type I collagen while maintains the homeostasis by inducing the apoptosis and necrosis of cells.

Özet

Amaç: Yüksek glikoza bağlı karaciğer fibrozis mekanizmalarını in vitro model kullanarak araştırmak amaçlanmıştır. Gereç ve yöntem: Yüksek glikoz konsantrasyonunun fibroblast proliferasyonu üzerindeki etkileri BrdU immünositokimya tekniği ile incelenmiştir. Apoptoz seviyeleri ve nekroz akım sitometri analizi ile tespit edildi. Kollajen tip I içeriği, ELISA ile ölçüldü. Bulgular: Yüksek glikoz düzeyi sadece NIH/3T3 fibroblast proliferasyonunu arttırmadı, aynı zamanda fibrozise benzer bir durumun göstergesi olarak tip I kollajen sentezini de arttırdı. Ayrıca yüksek glikoz, apoptoz ve nekroz seviyelerinin artmasına da neden olmuştur. Sonuç: Yüksek glikoz, NIH/3T3 fibroblast hücrelerinde fibrozisi, tip I kollajen üretimini indükleyerek modüle ederken, hücrelerin apoptoz ve nekrozunu indükleyerek homeostazı korur.

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Cite This Article

Soydaş, T., Saraç, E. Y., Çınar, S., Yenmiş, G., Doğan, S., Solakoğlu, S., Tunçdemir, M., Sultuybek, G. K. (2019). Effects of Short-term High Glucose on NIH/3T3 Fibroblast Proliferation, Apoptosis, and Collagen Type I Production. Tıp Fakültesi Klinikleri Dergisi, Cilt: 2 Sayı: 3, 91–95. https://ajindex.com/articles/1602174

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