Journals / Turkish Journal of Veterinary Research / 2026 / Volume: 10 Issue: 1

Determination of the effects of glucomannan supplementation into probiotic & enzyme combination on performance, some blood parameters and oxidative stress index in broilers

Mehmet Demirci(KIRIKKALE UNIVERSITY, DELİCE VOCATIONAL SCHOOL, DEPARTMENT OF VETERINARY, LABORATORY ASSISTANT TRAINING AND VETERINARY LABORATORY SERVICES PR.)0000-0002-0199-4559
Pages
—
Publication Date
2026-03-29
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, it was observed that the FCR, EPEF, carcass yield and data for heart, liver, pancreas, spleen and gizzard organs of the groups were similar between the groups (P>0.05), but a trend towards change was observed in the data for the bursa of Fabricius, and it was found to be higher in the PE and PEG groups compared to the control.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: This trial investigated the effects of adding plant-derived glucomannan to diets containing a probiotic and enzyme complex (PEx) on the performance and certain blood and oxidative stress parameters in broilers.
📊
Key Findings & Data:Materials and Methods: A total of 150-day-old male broiler chicks were used in the study, and three main groups were formed (one control (C), two experimental (named PE and PEG) groups).
💡
Conclusion & Impact:However, it was observed that the FCR, EPEF, carcass yield and data for heart, liver, pancreas, spleen and gizzard organs of the groups were similar between the groups (P>0.05), but a trend towards change was observed in the data for the bursa of Fabricius, and it was found to be higher in the PE and PEG groups compared to the control.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Broiler · Enzyme · Glucomannan

Abstract

Objective: This trial investigated the effects of adding plant-derived glucomannan to diets containing a probiotic and enzyme complex (PEx) on the performance and certain blood and oxidative stress parameters in broilers. Materials and Methods: A total of 150-day-old male broiler chicks were used in the study, and three main groups were formed (one control (C), two experimental (named PE and PEG) groups). Two TMR feed formulations were prepared for the broiler starter and grower periods. The complete diets were administered to the C group without any additives, to the PE group with 0.1% PEx added, and to the PEG group with both 0.1% PEx and additional plant glucomannan (Konjac gum) included. Results: According to the statistical data obtained at the end of the trial, it was determined that there were significant differences between the performance parameters BW, BWG, FI, and carcass weight values of the groups (P<0.05). These values were higher in the PEG group compared to the other two groups. However, it was observed that the FCR, EPEF, carcass yield and data for heart, liver, pancreas, spleen and gizzard organs of the groups were similar between the groups (P>0.05), but a trend towards change was observed in the data for the bursa of Fabricius, and it was found to be higher in the PE and PEG groups compared to the control. There were statistically significant differences between the groups in globulin, albumin and HDL cholesterol levels among the biochemical parameters (P<0.05). Globulin and HDL cholesterol levels were higher in the PE and PEG groups fed with supplemental diets, while albumin levels were lower. However, there were no significant differences between the groups in other biochemical parameters and oxidative stress (OS) indicators (TAS, TOS, and OS index) (P>0.05). Conclusion: It is thought that the combined use of PEx and glucomannan supplements at 0.1% levels in the diet produces notably positive effects, particularly on live performance parameters in broilers, and that these supplements have the potential for synergistic interaction. Additionally, it appears to contribute positively to organism health, particularly through its effects on increasing serum HDL cholesterol levels among biochemical parameters. However, more targeted studies are necessary to assess both the positive and potential negative effects on the immune system.

🔖

Cite This Article

Demirci, M. (2026). Determination of the effects of glucomannan supplementation into probiotic & enzyme combination on performance, some blood parameters and oxidative stress index in broilers. Turkish Journal of Veterinary Research, Volume: 10 Issue: 1. https://doi.org/10.47748/tjvr.1795359

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (54)

  1. Abdel-Raheem SM, Abd-Allah SM, Hassanein KM. The effects of prebiotic, probiotic and synbiotic supplementation on intestinal microbial ecology and histomorphology of broiler chickens. IJAVMS. 2012; 6(4):277-289.
  2. Adeola O, Cowieson AJ. Board-invited review: Opportunities and challenges in using exogenous enzymes to improve nonruminant animal production. J Anim Sci. 2011; 89(10):3189-3218.Al-Khalaifa H, Al-Nasser A, Al-Surayee T, et al. Effect of dietary probiotics and prebiotics on the performance of broiler chickens. Poult Sci. 2019; 98(10):4465-4479.
  3. Atuahene D, Sam BA, Idan F, et al. Probiotics, prebiotics, and synbiotics in pigs and poultry: a review of gut health, performance, and environmental outcomes. Vet Sci. 2025; 12(11):1054.
  4. AOAC. Official methods of analysis of AOAC International. 18th ed. Maryland: AOAC International; 2005.
  5. Azzaz HH, Murad HA, Hassaan NA, Fahmy M. Pectinase production optimization for improving dairy animal’s diets degradation. Int J Dairy Sci. 2019; 1:54-61.
  6. Behera SS, Ray RC. Konjac glucomannan, a promising polysaccharide of Amorphophallus konjac K. Koch in health care. Int J Biol Macromol. 2016; 92:942-956.
  7. Chua M, Baldwin TC, Hocking TJ, Chan K. Traditional uses and potential health benefits of Amorphophallus konjac K. Koch ex NE Br. J Ethnopharmacol. 2010; 128(2):268-278.
  8. Curea CD, Usturoi MG, Custură I, et al. Efficiency of Growing of Chicken Broilers Under Conditions of Compliance with Eu Rules of Welfare. Sci Pap-Ser D-Anim S. 2023; LXVI(1):273-278.
  9. Dong S, Li L, Hao F, et al. Improving quality of poultry and its meat products with probiotics, prebiotics, and phytoextracts. Poult Sci. 2024; 103(2):103287.
  10. Eckersall PD. Chapter 5: Proteins, proteomics, and the dysproteinemias. In: Kaneko JJ, Harvey JW, Bruss ML, eds. Clinical Biochemistry of Domestic Animals. 6th ed. Massachusetts: Academic Press; 2008. pp.117-155.
  11. Fijan S. Microorganisms with claimed probiotic properties: An overview of recent literature. Int J Environ Res Public Health. 2014; 11(5):4745-4767.
  12. Gaggìa F, Mattarelli P, Biavati B. Probiotics and prebiotics in animal feeding for safe food production. Int J Food Microbiol. 2010; 141:15-28.
  13. Güçlü BK, Kara K. Ruminant beslemede alternatif yem katkı maddelerinin kullanımı: 1. probiyotik, prebiyotik ve enzim. Ercivet. 2009; 6(1):65-75.
  14. Hardy H, Harris J, Lyon E, Beal J, Foey AD. Probiotics, prebiotics and immunomodulation of gut mucosal defences: homeostasis and immunopathology. Nutrients. 2013; 5(6):1869-1912.
  15. Hu JY, Mohammed AA, Murugesan GR, Cheng HW. Effect of a synbiotic supplement as an antibiotic alternative on broiler skeletal, physiological, and oxidative parameters under heat stress. Poult Sci. 2022; 101(4):101769.
  16. Jenkins G, Mason P. The role of prebiotics and probiotics in human health: a systematic review with a focus on gut and immune health. Food Nutr J. 2022; 7(3):245.
  17. Jha R, Mishra P. Dietary fiber in poultry nutrition and their effects on nutrient utilization, performance, gut health, and on the environment: a review. J Anim Sci Biotechnol. 2021; 12(1):1-16.
  18. Jian X, Jian S, Deng B. Konjac glucomannan: A functional food additive for preventing metabolic syndrome. J Funct Foods. 2024; 115:106108.
  19. Kamalzadeh A, Hosseini A, Moradi S. Effects of yeast glucomannan on performance of broiler chickens. Int J Agric Biol. 2009; 11(1):49-53.
  20. Kapoor DU, Sharma H, Maheshwari R, et al. Konjac glucomannan: A comprehensive review of its extraction, health benefits, and pharmaceutical applications. Carbohydr Polym. 2024; 339:122266.
  21. Kiani AK, Anpilogov K, Dautaj A, et al. Bacteriophages in food supplements obtained from natural sources. Acta Biomed. 2020; 91(13-S):e2020025.
  22. Kiarie E, Romero LF, Nyachoti CM. The role of added feed enzymes in promoting gut health in swine and poultry. Nutr Res Rev. 2013; 26(1):71-88.
  23. Kırkpınar F, Açıkgöz Z, Mert S, Işık Ö. Effects of dietary probiotic, prebiotic and enzyme mixture supplementation on performance, carcase, organs, ileal pH and viscosity of broilers. Hayüretim. 2018; 59(2):1-9.
  24. Khomayezi R, Adewole D. Probiotics, prebiotics, and synbiotics: an overview of their delivery routes and effects on growth and health of broiler chickens. Worlds Poult Sci J. 2022; 78(1):57-81.
  25. Larasati YN, Harmayani E, Widada J, Nurliyani N, Perdinan A. Caecal bacterial composition of broiler chickens affected by porang glucomannan. J Indonesian Trop Anim Agric. 2021; 46(3):187-198.
  26. Luo C, Wan L, Chen Y, Yuan J. Supplemental enzyme and probiotics on the growth performance and nutrient digestibility of broilers fed with a newly harvested corn diet. Animals. 2022; 12(18):2381.
  27. Maftei N-M, Raileanu CR, Balta AA, et al. The potential impact of probiotics on human health: an update on their health-promoting properties. Microorganisms. 2024; 12(2):234.
  28. Markowiak P, Śliżewska K. Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients. 2017; 9(9):1021.
  29. Mavromati E, Sena L, Gjeta Z, Mavromati J. Assessing the economic efficiency in some broiler farms through the European production efficiency factor (EPEF). EAR. 2018; 6(9):5354-5362.
  30. Meijerink N, De Oliveira JE, Van Haarlem DA, et al. Glucose oligosaccharide and long-chain glucomannan feed additives induce enhanced activation of intraepithelial NK cells and relative abundance of commensal lactic acid bacteria in broiler chickens. Vet Sci. 2021; 8(6):110.
  31. Mountzouris KC, Tsirtsikos P, Kalamara E, Nitsch S, Schatzmayr G, Fegeros K. Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult Sci. 2007; 86(2):309-317.
  32. Murthy TNK, Reddy BN, Devegowda G. Evaluation of glucomannan for its adsorbing ability of aflatoxin B1 and T-2 Toxin in the gastrointestinal tract of broiler chickens. Mycotoxin Res. 2002; 18(Suppl 1):20-23.
  33. Naeem M, Bourassa D. Probiotics in poultry: Unlocking productivity through microbiome modulation and gut health. Microorganisms. 2025; 13(2):257.
  34. NRC. National Research Council. Nutrient requirements of poultry. 9th Rev Ed. Washington: National Academy Press; 1994.
  35. Ogwiji M, Jatau ID, Natala AJ, Mohany M, Al-Rejaie SS, Zhu M. Effect of prebiotic, probiotic, and synbiotic products on oxidative status, performance, and parasitological parameters of broiler chickens induced with cecal coccidiosis. J Appl Poult Res. 2024; 33(4):100472.
  36. Oleskin AV, Boyang C. Microalgae in terms of biomedical technology: Probiotics, prebiotics, and metabiotics. Appl Biochem Microbiol. 2022; 58(6):813-825.
  37. Oliveira EM, Tanure CBGS, Castejon FV, et al. Performance and nutrient metabolizability in broilers fed diets containing corn contaminated with Fumonisin B1 and esterified glucomannan. Braz J Poult Sci. 2015; 17(3):313-318.
  38. Ölmez M, Şahin T, Karadağoğlu Ö, et al. Effects of dietary prebiotic, probiotic, and synbiotic supplementation on growth performance, carcass traits, digestive enzymes and some biochemical parameters in broiler chickens. J Hellenic Vet Med Soc. 2025; 76(1):8705-8714.
  39. Patterson JA, Burkholder KM. Application of prebiotics and probiotics in poultry production. Poult Sci. 2003; 82(4):627-631.
  40. Perdinan A, Wahyuni HI, Suthama N. Body resistance and growth performance of broiler fed glucomannan extracted from Amorphophallus onchophyllus tuber. Trop Anim Sci J. 2019; 42(1):33-38.
  41. Rehman A, Arif M, Sajjad N, et al. Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity. Poult Sci. 2020; 99(12):6946-6953.
  42. Saiyed MA, Joshi RS, Savaliya FP, Patel AB, Mishra RK, Bhagora NJ. Study on inclusion of probiotic, prebiotic and its combination in broiler diet and their effect on carcass characteristics and economics of commercial broilers. Vet World. 2015; 8(2):225.
  43. Sarangi NR, Babu LK, Kumar A, Pradhan CR, Pati PK, Mishra JP. Effect of dietary supplementation of prebiotic, probiotic, and synbiotic on growth performance and carcass characteristics of broiler chickens. Vet World. 2016; 9(3):313.
  44. Song D, Ibrahim S, Hayek S. Recent application of probiotics in food and agricultural science. Probiotics. 2012; 10:1-34.
  45. SPSS. SPSS 15.0 for Windows Evaluation Version. The SPSS base 15.0 user’s guide. SPSS Inc. Chicago, USA; 2006.
  46. Swanson KS, Allenspach K, Amos G, et al. Use of biotics in animals: impact on nutrition, health, and food production. J Anim Sci. 2025; 103:skaf061.
  47. Swanson KS, Gibson GR, Hutkins R, et al. The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of synbiotics. Nat Rev Gastro Hepat. 2020; 17(11):687-701.
  48. Tomczyk G, Niczyporuk JS, Kozdruń W, et al. Probiotic supplementation as an alternative to antibiotics in broiler chickens. J. Vet. Res. 2024; 68(1):147.
  49. Van Limbergen T, Sarrazin S, Chantziaras I, et al. Risk factors for poor health and performance in European broiler production systems. BMC Vet Res. 2020; 16(1):287.
  50. Webb K, Peterson J, Fox S, et al. Effect of prebiotic, probiotic, and enzyme supplementation on gut fermentation, markers of inflammation and immune response in individuals with GI Symptoms. Curr Dev Nutr. 2019; 3:04-20.
  51. Xing W, Gao W, Lv X, et al. The effects of supplementation of probiotics, prebiotics, or synbiotics on patients with non-alcoholic fatty liver disease: A meta-analysis of randomized controlled trials. Front Nutr. 2022; 9:1024678.
  52. Yıldız A, Şentürk ET, Olgun O. The effect of multi-enzyme addition to quail diets on performance, egg quality and serum parameters. TURJAF. 2021; 9(3):536-541.
  53. Żbikowski A, Pawłowski K, Śliżewska K, Dolka B, Nerc J, Szeleszczuk P. Comparative effects of using new multi-strain synbiotics on chicken growth performance, hematology, serum biochemistry and immunity. Animals. 2020; 10(9):1555.
  54. Zhao Z, Liswaniso S, Qin N, Cao S, Wu X, Ma C, et al. Effects of a novel synbiotics-enzyme complex as a replacement for antibiotics on growth performance, slaughter and meat characteristics, immune organ index, and intestinal morphology of broilers. Front Vet Sci. 2024; 11:1468847.