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Journals / Turkish Journal of Kinesiology / 2025 / Volume: 11 Issue: 6

Circadian rhythm–dependent effects of acute caffeine consumption on hydration and performance in football players

Özgür Taylan Ökke(Department of the Coaching Education, Graduate School of Education, University of Sinop)0009-0003-4503-8985
Ahmet Mor(Department of the Coaching Education, Faculty of Sport Sciences, University of Sinop)0000-0002-1181-1111
Pages
—
Publication Date
2025-12-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, this study revealed that acute CAF intake in relation to circadian rhythm had no significant effect on performance in football players.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to investigate the effects of acute caffeine (CAF) consumption at different time-of-day, in relation to circadian rhythm, on selected performance parameters and hydration status in football players, as well as to assess gastrointestinal responses following CAF intake.
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Key Findings & Data:The study included twelve trained male football players aged between 18-25 years.
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Conclusion & Impact:In conclusion, this study revealed that acute CAF intake in relation to circadian rhythm had no significant effect on performance in football players.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Caffeine · circadian rhythm · football players

Abstract

The aim of this study was to investigate the effects of acute caffeine (CAF) consumption at different time-of-day, in relation to circadian rhythm, on selected performance parameters and hydration status in football players, as well as to assess gastrointestinal responses following CAF intake. The study included twelve trained male football players aged between 18-25 years. Within the experimental design, which employed a randomized, single-blind approach, the familiarization session was conducted on the first day, followed by three separate test days. Sixty minutes before the initiation of testing, the football players ingested CAF at a dose of 6 mg/kg⁻¹. The tests, measurements, and analyses were conducted at 10:00 a.m. on day 1, 14:00 p.m. on day 2, and 18:00 p.m. on day 3. Differences between variables were analyzed using repeated-measures ANOVA. No significant difference was found in vertical jump, anaerobic power, change of direction (COD), 10 and 20m sprint, ball speed, rating of perceived exertion (RPE), and visual analogue scale (VAS) between time-of-day after CAF intake (p>0.05). There were significant main effects for the 30m sprint between time-of-day after CAF intake (p 0.05). There was a significant difference in the Gastrointestinal Symptom Rating Scale (GSRS) total between the time-of-day after CAF intake (p<0.05). In conclusion, this study revealed that acute CAF intake in relation to circadian rhythm had no significant effect on performance in football players. Additionally, the findings indicated a slight decline in performance during the morning hours. This outcome is thought to be primarily attributable to the athletes’ habitual training and competition schedules rather than to CAF intake. Furthermore, gastrointestinal symptoms associated with morning CAF intake may have contributed to the observed performance decrease. Overall, the data suggested a tendency toward more favorable outcomes in the evening measurements.

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Cite This Article

Ökke, Ö. T., Mor, A. (2025). Circadian rhythm–dependent effects of acute caffeine consumption on hydration and performance in football players. Turkish Journal of Kinesiology, Volume: 11 Issue: 6. https://doi.org/10.31459/turkjkin.1786020

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References (36)

  1. Albersnagel, F. A. (1988). Velten and music mood induction procedures: A comparision with accessibility of thought associations. Behav Res Ther, 26(1), 79-95. doi: 10.1016/0005-7967(88)90035-6
  2. Alpar, R. (2022). Spor, sağlık ve eğitim bilimlerinden örneklerle uygulamalı istatistik ve geçerlilik-güvenirlik. Ankara: Detay Publishing. (in Turkish)
  3. Bangsbo, J. (2014). Physiological demands of football. Sports Science Exchange, 27(125), 1-6.
  4. Bangsbo, J., Mohr, M., & Krustrup, P. (2006). Physical and metabolic demands of training and match-play in the elite football player. J Sports Sci, 24(7), 665-674. doi: 10.1080/02640410500482529
  5. Behrens, M., Mau-Moeller, A., Weippert, M., Fuhrmann, J., Wegner, K., Skripitz, R., Bader, R., & Bruhn, S. (2015). Caffeine-induced increase in voluntary activation and strength of the quadriceps muscle during isometric, concentric and eccentric contractions. Sci Rep, 5, 10209. doi: 10.1038/srep10209
  6. Borg, G. (1998). Borg's perceived exertion and pain scales. Champaign, IL: Human Kinetics.
  7. Bougrine, H., Ammar, A., Salem, A., Trabelsi, K., Żmijewski, P., Jahrami, H., Chtourou, H., & Souissi, N. (2024). Effects of different caffeine dosages on maximal physical performance and potential side effects in low-consumer female athletes: Morning vs. evening administration. Nutrients, 16(14), 2223. doi: 10.3390/nu16142223
  8. Bougrine, H., Cherif, M., Chtourou, H., & Souissi, N. (2022). Can caffeine supplementation reverse the impact of time of day on cognitive and short-term high intensity performances in young female handball players? Chronobiol Int, 39(8), 1144-1155. doi: 10.1080/07420528.2022.2077747
  9. Boyett, J. C., Giersch, G. E., Womack, C. J., Saunders, M. J., Hughey, C. A., Daley, H. M., & Luden, N. D. (2016). Time of day and training status both impact the efficacy of caffeine for short duration cycling performance. Nutrients, 8(10), 639. doi.org/10.3390/nu8100639
  10. Davis, J., & Green, J. M. (2009). Caffeine and anaerobic performance. Sports Med, 39(10), 813-832. doi: 10.2165/11317770-000000000-00000
  11. Drăgoi, C. M., Nicolae, A. C., Ungurianu, A., Margină, D. M., Grădinaru, D., & Dumitrescu, I. B. (2024). Circadian rhythms, chrononutrition, physical training, and redox homeostasis—molecular mechanisms in human health. Cells, 13(2), 138. doi: 10.3390/cells13020138
  12. Fox, E., Bowers, R., & Foss, M. (2012). Beden eğitimi ve sporun fizyolojik temelleri. Ankara: Spor Publishing. (in Turkish)
  13. Graham, T. E., & Spriet, L. L. (1995). Metabolic, catecholamine, and exercise performance responses to various doses of caffeine. J Appl Physiol (1985), 78(3), 867-874. doi: 10.1152/jappl.1995.78.3.867
  14. Grgic, J., & Del Coso, J. (2021). Ergogenic effects of acute caffeine intake on muscular endurance and muscular strength in women: A meta-analysis. Int J Environ Res Public Health, 18(11), 5773. doi: 10.3390/ijerph18115773
  15. Haupt, S., Eckstein, M. L., Wolf, A., Zimmer, R. T., Wachsmuth, N. B., & Moser, O. (2021). Eat, train, sleep—retreat? Hormonal interactions of intermittent fasting, exercise and circadian rhythm. Biomolecules, 11(4), 516. doi: 10.3390/biom11040516
  16. Kline, C. E., Durstine, J. L., Davis, J. M., Moore, T. A., Devlin, T. M., Zielinski, M. R., & Youngstedt, S. D. (2007). Circadian variation in swim performance. J Appl Physiol (1985), 102(2), 641-649. doi: 10.1152/japplphysiol.00910.2006
  17. Lopes-Silva, J. P., Santos, J. F. D. S., & Franchini, E. (2019). Can caffeine supplementation reverse the effect of time of day on repeated-sprint exercise performance? Appl Physiol Nutr Metab, 44(2), 187-193. doi: 10.1139/apnm-2018-0373
  18. Mor, A., Acar, K., Alexe, D. I., Mor, H., Abdioğlu, M., Man, M. C., Karakaș, F., Waer, F. B., Yılmaz, A. K., & Alexe, C. I. (2024). Moderate-dose caffeine enhances anaerobic performance without altering hydration status. Front Nutr, 11, 1359999. doi: 10.3389/fnut.2024.1359999
  19. Mor, A., Karakaş, F., Mor, H., Yurtseven, R., Yılmaz, A. K., & Acar, K. (2022). The effects of resistance band exercises on some performance parameters in young football players. Spormetre The Journal of Physical Education and Sport Sciences, 20 (3), 128-142. doi: 10.33689/spormetre.1095371
  20. Mor, A., Yurtseven, R., Mor, H., & Acar, K. (2021). The effects of different warm-up protocols on some performance parameters in 11-12 age group football players. Spormetre The Journal of Physical Education and Sport Sciences, 19 (4), 72-83. doi: 10.33689/spormetre.907920
  21. Mor, H., Mor, A., Abdioğlu, M., Tohănean, D. I., Savu, C. V., Acar, G. C., Moraru, C. E., & Alexe, D. I. (2025). The acute effects of caffeine supplementation on anaerobic performance and functional strength in female soccer players. Nutrients, 17(13), 2156. doi.org/10.3390/nu17132156
  22. Mora-Rodriguez, R., Pallares, J. G., Lopez-Gullon, J. M., Lopez-Samanes, A., Fernandez-Elias, V. E., & Ortega, J. F. (2015). Improvements on neuromuscular performance with caffeine ingestion depend on the time-of-day. J Sci Med Sport, 18(3), 338-342. doi: 10.1016/j.jsams.2014.04.010
  23. Mora-Rodríguez, R., Pallarés, J. G., López-Samanes, Á., Ortega, J. F., & Fernández-Elías, V. E. (2012). Caffeine ingestion reverses the circadian rhythm effects on neuromuscular performance in highly resistance-trained men. PLoS One, 7(4), e33807. doi: 10.1371/journal.pone.0033807
  24. Okudur, A., & Sanioğlu, A. (2012). The relationship between balance and agility performance in tennis players aged 12. Selçuk University Journal of Physical Education and Sport Science, 14(2), 165–170.
  25. Racinais, S., Blonc, S., & Hue, O. (2005). Effects of active warm-up and diurnal increase in temperature on muscular power. Med Sci Sports Exerc, 37(12), 2134-2139. doi: 10.1249/01.mss.0000179099.81706.11
  26. Revicki, D.A., Wood, M., Wiklund, I., & Crawley, J. (1997). Reliability and validity of the gastrointestinal symptom rating scale in patients with gastroesophageal reflux disease. Qual Life Res, 7(1), 75–83. doi.org/10.1023/a:1008841022998
  27. Sedliak, M., Finni, T., Peltonen, J., & Hakkinen, K. (2008). Effect of time-of-day-specific strength training on maximum strength and EMG activity of the leg extensors in men. J Sports Sci, 26(10), 1005-1014. doi: 10.1080/02640410801930150
  28. Sherman, H., Gutman, R., Chapnik, N., Meylan, J., Le Coutre, J., & Froy, O. (2011). Caffeine alters circadian rhythms and expression of disease and metabolic markers. Int J Biochem Cell Biol, 43(5), 829-838. doi: 10.1016/j.biocel.2011.02.008
  29. Souissi, M., Abedelmalek, S., Chtourou, H., Atheymen, R., Hakim, A., & Sahnoun, Z. (2012). Effects of morning caffeine’ingestion on mood States, simple reaction time, and short-term maximal performance on elite judoists. Asian J Sports Med, 3(3), 161. doi: 10.5812/asjsm.34607
  30. Souissi, M., Abedelmalek, S., Chtourou, H., Boussita, A., Hakim, A., & Sahnoun, Z. (2013). Effects of time-of-day and caffeine ingestion on mood states, simple reaction time, and short-term maximal performance in elite judoists. Biological Rhythm Research, 44(6), 897-907. doi: 10.1080/09291016.2013.780700
  31. Souissi, M., Aloui, A., Chtourou, H., Aouicha, H. B., Atheymen, R., & Sahnoun, Z. (2015). Caffeine ingestion does not affect afternoon muscle power and fatigue during the Wingate test in elite judo players. Biological Rhythm Research, 46(2), 291-298. doi: 10.1080/09291016.2014.994857
  32. Souissi, N., Driss, T., Chamari, K., Vandewalle, H., Davenne, D., Gam, A., Fillard, J.-R., & Jousselin, E. (2010). Diurnal variation in Wingate test performances: Influence of active warm-up. Chronobiol Int, 27(3), 640-652. doi: 10.3109/07420528.2010.483157
  33. Souissi, Y., Souissi, M., & Chtourou, H. (2019). Effects of caffeine ingestion on the diurnal variation of cognitive and repeated high-intensity performances. Pharmacol Biochem Behav, 177, 69-74. doi: 10.1016/j.pbb.2019.01.001
  34. Tarnopolsky. (2008). Effect of caffeine on the neuromuscular system--potential as an ergogenic aid. Appl Physiol Nutr Metab, 33(6), 1284-1289. doi: 10.1139/H08-121
  35. Tessitore, A., Meeusen, R., Piacentini, M., Demarie, S., & Capranica, L. (2006). Physiological and technical aspects of" 6-a-side" soccer drills. J Sports Med Phys Fitness, 46(1), 36.
  36. Zhang, Y., Yang, W., Xue, Y., Hou, D., Chen, S., Xu, Z., Peng, S., Zhao, H., Wang, C., & Liu, C. (2024). Timing matters: Time of day impacts the ergogenic effects of caffeine—A narrative review. Nutrients, 16(10), 1421. doi: 10.3390/nu16101421