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Journals / Turkish Journal of Biodiversity / 2025 / Cilt: 8 Sayı: 2

Quantitative analysis of Medicago lupulina stem and evaluation of antioxidant activity

Medicago lupulina gövdesinin kantitatif analizi ve antioksidan aktivitesinin değerlendirilmesi

Ramazan Erenler(Iğdır Üniversitesi)0000-0002-0505-3190
İlyas Yıldız(TOKAT GAZIOSMANPASA UNIVERSITY)0000-0003-1254-1069
Esma Nur Geçer(TOKAT GAZIOSMANPASA UNIVERSITY)0000-0002-0095-079X
İbrahim Hosaflıoğlu(IĞDIR ÜNİVERSİTESİ, IĞDIR ZİRAAT FAKÜLTESİ)0009-0000-2730-1493
Pages
74–80
Publication Date
2025-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Identifying bioactive compounds in M. lupulina stems at high concentrations will significantly contribute to studies aimed at their isolation.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Plants have gained considerable interest in drug discovery owing to their rich content of bioactive secondary metabolites, which play a critical role in the pharmaceutical industry.
📊
Key Findings & Data:A significant proportion of currently used therapeutic agents are derived from naturally occurring biologically active compounds.
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Conclusion & Impact:Identifying bioactive compounds in M. lupulina stems at high concentrations will significantly contribute to studies aimed at their isolation.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Kantitatif analiz · Medicago lupulina gövde · antioksidan aktivite

Abstract

Plants have gained considerable interest in drug discovery owing to their rich content of bioactive secondary metabolites, which play a critical role in the pharmaceutical industry. A significant proportion of currently used therapeutic agents are derived from naturally occurring biologically active compounds. In this study, the phenolic composition of Medicago lupulina stem was quantified by LC–MS/MS, revealing salicylic acid as the predominant phenolic compound (78.4 µg/g extract). The antioxidant potential of the methanolic stem extract was evaluated using the DPPH and ABTS assays. The extract exhibited notable antioxidant activity, with IC50 values of 14.8 µg/mL and 8.7 µg/mL in the DPPH and ABTS assays, respectively. Identifying bioactive compounds in M. lupulina stems at high concentrations will significantly contribute to studies aimed at their isolation.

Özet

Bitkiler, ilaç endüstrisinde önemli rol oynayan zengin biyoaktif ikincil metabolit içerikleri nedeniyle ilaç keşfinde yoğun ilgi görmektedir. Günümüzde kullanılan terapötik ajanların önemli bir kısmı, doğal olarak oluşan aktif bileşiklerden elde edilmektedir. Bu çalışmada, Medicago lupulina gövdesinin fenolik bileşimi LC-MS/MS ile kantitatif olarak belirlenmiş ve ana ürün olarak salisilik asit (78,4 µg/g ekstrakt) tespit edilmiştir. Metanol ekstraktının antioksidan potansiyeli, DPPH ve ABTS testleri kullanılarak değerlendirilmiştir. Ekstrakt, DPPH ve ABTS testlerinde sırasıyla 14,8 µg/mL ve 8,7 µg/mL IC50 değerleriyle kayda değer antioksidan aktivite göstermiştir. M. lupulina saplarında yüksek konsantrasyonlarda bulunan biyoaktif bileşiklerin tanımlanması, bunların izolasyonuna odaklanan çalışmalara önemli katkı sağlayacaktır.

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Cite This Article

Erenler, R., Yıldız, İ., Geçer, E. N., Hosaflıoğlu, İ. (2025). Quantitative analysis of Medicago lupulina stem and evaluation of antioxidant activity. Turkish Journal of Biodiversity, Cilt: 8 Sayı: 2, 74–80. https://doi.org/10.38059/biodiversity.1843090

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