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Journals / Sağlık Bilimleri Dergisi / 2009 / Cilt: 18 - Sayı: 2

Effects of Melatonin and Vitamin C on Damage and Expression of Endothelial NOS in Cerebellum of Chronic Alcoholic Rats

MELATONİN VE C VİTAMİNİNİN KRONİK ALKOLİK SIÇANLARIN SEREBELLUM DOKUSUNDAKİ HASAR VE eNOS İMMUNREAKTİVİTESİ ÜZERİNE ETKİLERİ

Mehmet Fatih SÖNMEZ
Derya AKKUŞ
Pages
62–71
Publication Date
2009-06-01
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

In melatonin andvitamin C treated groups, eNOS immunoreactivitywas (+++).Present results indicated that chronic alcoholconsumption increased lipid peroxidation andespecially melatonin and vitamin C administrationproduced in some degree protection against alcoholinduced damage in cerebellum

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to determine thealterations in cerebellum caused by alcoholconsumption and the effects of melatonin and vitaminC on these changes, in rats.Twenty-four adult male Wistar rats weighting 200250g were used in this study.
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Key Findings & Data:The first group served as control (n:6).The second group was the ethanol treatment for 28days (n:6).
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Conclusion & Impact:In melatonin andvitamin C treated groups, eNOS immunoreactivitywas (+++).Present results indicated that chronic alcoholconsumption increased lipid peroxidation andespecially melatonin and vitamin C administrationproduced in some degree protection against alcoholinduced damage in cerebellum
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Kronik alkol tüketimi · beyincik · eNOS

Abstract

The aim of this study was to determine thealterations in cerebellum caused by alcoholconsumption and the effects of melatonin and vitaminC on these changes, in rats.Twenty-four adult male Wistar rats weighting 200250g were used in this study. Rats were divided intofour groups. The first group served as control (n:6).The second group was the ethanol treatment for 28days (n:6). The third group was given ethanol andsupplemented (intraperitoneally) (n:6). The fourth group was givenethanol and supplemented with 4 mg/kg/daymelatonin (intraperitoneally) (n:6).Light microscopic examinations revealed Purkinjecell degeneration and decrease in the number ofPurkinje cells in ganglionic layer of cerebellum. Inmelatonin and vitamin C treated groups, degenerationin Purkinje cells was less than the other groups. In thecontrol group, endothelial nitric oxide synthaseimmunoreactivity was (+++) in Purkinje cells, and(++) in granular and molecular layer. No endothelialnitric oxide synthase activity in degenerated Purkinjecells of alcohol group was observed. In melatonin andvitamin C treated groups, eNOS immunoreactivitywas (+++).Present results indicated that chronic alcoholconsumption increased lipid peroxidation andespecially melatonin and vitamin C administrationproduced in some degree protection against alcoholinduced damage in cerebellum

Özet

Bu çalışmada etanolün serebellumda oluşturduğuhasar ve eNOS ekspresyonu üzerine melatonin ve Cvitamininin amaçlandı.Çalışmada 24 adet 200-250 gr ağırlığında Wistar cinsiergin erkek sıçan kullanıldı. Sıçanlar rasgele olarakdört gruba ayrıldı. Birinci grup kontrol (n: 6); ikincigrup deney süresince (28 gün) alkol içeren sıvı diyetalan (n: 6); üçüncü grup deney boyunca alkol içeren sıvıdiyet ile birlikte 40mg/kg/gün intraperitoneal C vitaminialan (n: 6); dördüncü grup deney süresince alkol içerensıvı diyet ile birlikte 4mg/kg/gün intraperitonealmelatonin (n: 6) alan sıçanlardan oluşturuldu.Deney süresince sadece alkol alan gruptaki sıçanlarınserebellum dokularında gangliyoner tabakada purkinjehücrelerinde dejenerasyon ve hücre sayılarında azalmagözlendi. Melatonin ve C vitamini uygulanan gruplardapurkinje hücrelerindeki dejenerasyon oranının azaldığıayırt edildi. Kontrol grubu sıçanlarda, endotelyal nitrikoksit sentaz immunoreaktivitesi için yapılan boyamadamoleküler ve granüler tabakalarda (++) boyanma,purkinje hücrelerinde ise (+++) boyanma gözlendi.Alkol alan grupta dejenere olan purkinje hücrelerindeendotelyal nitrik oksit sentaz immunoreaktivitesigözlenmedi. Melatonin ve C vitamini uygulanangruplarda ise (+++) boyanma mevcuttu.Sonuç olarak kronik alkol tüketimi serebellumdokusunda hasara yol açmakta. Bu hasar melatonin veC vitamini ile kısmen düzelmektedir

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Cite This Article

SÖNMEZ, M. F., AKKUŞ, D. (2009). Effects of Melatonin and Vitamin C on Damage and Expression of Endothelial NOS in Cerebellum of Chronic Alcoholic Rats. Sağlık Bilimleri Dergisi, Cilt: 18 - Sayı: 2, 62–71. https://ajindex.com/articles/155425

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