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Journals / New Trends in Medicine Sciences / 2021 / Volume: 2 Issue: 2

Biochemical and Histopathological Evaluation of the Protective Efficacy of Thymoquinone in Experimentally Ischemia Reperfusion Induced Rat Ovaries

Özge Nur Türkeri(LEFKE AVRUPA ÜNİVERSİTESİ)0000-0001-8791-5331
Ayhan Tanyeli(ATATURK UNIVERSITY)0000-0002-0095-0917
Nezahat Kurt(ERZINCAN BINALI YILDIRIM UNIVERSITY)0000-0002-1685-5332
Nuri Bakan(ATATURK UNIVERSITY)0000-0002-2139-7268
Fazile Nur Ekinci Akdemir(AGRI IBRAHIM CECEN UNIVERSITY)0000-0001-9585-3169
Behzat Mokhtare(ATATURK UNIVERSITY)
Pages
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Publication Date
2021-09-15
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: It was concluded that thymoquinone protected the tissues from ischemic damage by causing significant increase in TAS levels that were decreased by I/R injury and significant decrease in elevated levels of MDA and TOS that were increased by I/R injury.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: In our study, oxidant-antioxidant and inflammation markers of ovarian tissue were evaluated in order to determine whether thymoquinone has a protective effect against experimentally induced ischemia-reperfusion in rat ovaries.
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Key Findings & Data:Material and Method: The rats, which randomly divided into 5 groups and were experimentally induced ischemia-reperfusion in their ovaries, were administered timokinon at the determined doses.
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Conclusion & Impact:Conclusion: It was concluded that thymoquinone protected the tissues from ischemic damage by causing significant increase in TAS levels that were decreased by I/R injury and significant decrease in elevated levels of MDA and TOS that were increased by I/R injury.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Ischemia Reperfusion · Oxidative stress · Thymoquinone

Abstract

Aim: In our study, oxidant-antioxidant and inflammation markers of ovarian tissue were evaluated in order to determine whether thymoquinone has a protective effect against experimentally induced ischemia-reperfusion in rat ovaries. In our study, oxidant-antioxidant and inflammation markers in ovarian tissue were evaluated in order to determine whether thymoquinone has a protective effect against damage in rat ovaries in which ischemia-reperfusion was created. Material and Method: The rats, which randomly divided into 5 groups and were experimentally induced ischemia-reperfusion in their ovaries, were administered timokinon at the determined doses. The markers such as TAS, TOS and MDA from ovarian tissues were determined as spectrophotometrically obtained by experimental procedure. Tissues examined histopathologically under light microscopy were evaluated for immunopositivity after immunohistochemical staining with NF-Kβ1 antibodies. Results: While MDA, TOS, OSI levels in tissue were significantly higher in ischemia/reperfusion groups, and TQ, MDA, TOS, OSI levels were significantly lower rather than the control group (p 0.05). Conclusion: It was concluded that thymoquinone protected the tissues from ischemic damage by causing significant increase in TAS levels that were decreased by I/R injury and significant decrease in elevated levels of MDA and TOS that were increased by I/R injury.

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Cite This Article

Türkeri, Ö. N., Tanyeli, A., Kurt, N., Bakan, N., Akdemir, F. N. E., Mokhtare, B. (2021). Biochemical and Histopathological Evaluation of the Protective Efficacy of Thymoquinone in Experimentally Ischemia Reperfusion Induced Rat Ovaries. New Trends in Medicine Sciences, Volume: 2 Issue: 2. https://ajindex.com/articles/1532546

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References (34)

  1. Meyer JS, Harmon CM, Harty MP, Markowitz RI, Hubbard AM, Bellah RD. Ovarian torsion: clinical and imaging presentation in children. Journal of Pediatric Surgery, 1995, 30: 1433- 1436.
  2. Chen M, Chen CD, Yang YS. Torsion of the previously normal uterine adnexa. Evaluation of the correlation between the pathological changes and the clinical characteristics. Acta Obstetricia et Gynecologica Scandinavica, 2001, 80: 58-61.
  3. Haskins T, Shull BL. Adnexal torsion: a mind-twisting diagnosis. Southern Medical Journal, 1986, 79: 576-577.
  4. Taskin O, Birincioglu M, Aydin A, Buhur A, Burak F, Yılmaz I, Wheeler JM. The effects of twisted ischaemic adnexa managed by detorsion on ovarian viability and histology: an ischaemia-reperfusion rodent model. Hum Reprod, 1998, 13: 2823- 2827
  5. Kuroda S, Siesjo BK. Reperfusion damage following focal ischemia: pathophysiology and therapeutic windows. Clinical neuroscience, 1997, 4: 199- 212.
  6. Jordan JE, Zhao ZQ, Vinten-Johansen J. The role of neutrophils in myocardial ischemia-reperfusion injury. Cardiovascular Research, 1999, 43: 860-878.
  7. Mogilner JG, Lurie M, Coran AG, Nativ O, Shiloni E, Sukhotnik I. Effect of diclofenac on germ cell apoptosis following testicular ischemia-reperfusion injury in a rat. Pediatric Surgery International, 2006, 22: 99-105
  8. Medenica R, Mukerjee S, Muschart T, Koffskey J, Corbit W. Nigella sativa plant extract increases number and activity of immune component cell in humans. Experimental Hematology, 1993, 21 (3): 1186.
  9. Aggarwal BB, Kunnumakkara AB, Harikumar KB, Tharakan ST, Sung B. Anand P. Potential of spice-derived phytochemicals for cancer prevention. Planta Medica, 2008, 74 (13): 1560-1569.
  10. Staicu M.L., Mureşan A., Tache S., Moldovan R. 2011. Effects of exogenous antioxidants on oxidative stress in pregnancy. Journal of Medicine and Life; 4, 2, 163-167.)
  11. Abdollahi, M., Ranjbar, A., Shadnia, S., Nikfar, S., Rezaie, A., 2004. Pesticides and oxidative stress: a review. Medical Science Monitor; 10, 6, 141-147.)
  12. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem.).
  13. Ohkawa H,Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbutric acid reaction. Analytical Biochemistry, 1979, 95:351-358.
  14. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004;37:112–119.
  15. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38:1103–1111.
  16. Dorweiler B, Pruefer D, Andrasi TB, Maksan SM, Schmiedt W, Neufang A, Vahl CF. Ischemia-Reperfusion Injury Pathophysiology and Clinical Implications. European Journal of Trauma and Emergency Surgery, 2007, 33: 600-612.
  17. Zimmerman BJ, Granger DN. Reperfusion injury. Surgical Clinics of North America, 1992, 72: 65-83.
  18. Cerqueira NF, Hussni CA, Yoshida WB. Pathophysiology of mesenteric ischemia/reperfusion: a review. Acta Cirurgica Brasileira, 2005, 20: 336-343.
  19. Cakir Gungor AN, Gencer M, Karaca T, et al. The effect of hesperetin on ischemia-reperfusion injury in rat ovary. Arch Gyne col Obstet, 2014, 290: 763-769.
  20. Sahin FK, Cosar E, Koken G, et al. Protective effect of aprotinin on ischemia-reperfusion injury in rat ovary. J Obstet Gynaecol Res, 2008, 34: 794-800.
  21. Ustundag UV, Sahin S, Ak K,et al. The effects of tacrolimus on the activity and expression of tissue factor in the rat ovary with ischemia-reperfusion induced injury. Reprod Biol, 2015, 15: 139-145.
  22. Akdemir A, Sahin C, Erbas O, et al. Isursodeoxycholic acid crucial for ischemia / reperfusion-induced ovarian injury in rat ovary? Arch Gynecol Obstet, 2015, 292: 445-450
  23. Kılıç A., Uyanıkoğlu H., İncebıyık A. Protective Effect of N-Acetylcystein and Resveratrol on Ischemia-Reperfusion Injury in Rat Ovary. Dicle Medical Journal, 2016, 43(2): 229-236
  24. Ergun Y, Koc A, Dolapcioglu K, et al. The protective effect of erythropoietin and dimethylsulfoxide on ischemia-reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol, 2010, 152: 186-190.
  25. Eser A, Hizli D, Haltas H, et al. Effects of curcumin on ovarian ischemia- reperfusion injury in a rat model. Biomed Rep, 2015, 3: 807-813.
  26. Celik M. Sildenafilin Sıçan Överlerinde Oluşturulan İskemi Reperfüzyon Hasarındaki Etkisinin Biyokimyasal Ve Histopatolojik Olarak Araştırılması. Tıp fakültesi, Biyokimya Anabilim Dalı. Uzmanlık Tezi, Erzurum: Atatürk Üniversitesi, 2011.
  27. Hussein AE, Abd-Elkhabir A, Abozahra A, Baiomy A, Ashamallah SA, Sheashaa HA, Sobh MA. Pancreatic injury secondary to renal ischemia/reperfusion (I/R) injury: possible role of oxidative stress. Physiological research / Academia Scientiarum Bohemoslovaca, 2013.
  28. Kowalczuk K, Stryjecka-Zimmer M. The influence of oxidative stress on the level of malondialdehyde (MDA) in different areas of the rabbit brain. Annales Universitatis Mariae Curie-Sklodowska. Sectio D: Medicina, 2002, 57: 160-164
  29. Yuan GJ, Ma JC, Gong ZJ, et al. Modulation of liver oxidant-antioxidant system by ischemic preconditioning during ischemia/reperfusion injury in rats. World J Gastroenterol, 2005, 11: 1825-1828.
  30. Sancak EB, Akbas A, Silan C, Cakir DU, Turkon H, Ozkanli SS. Protective effect of syringic acid on kidney ischemia-reperfusion injury. Ren Fail, 2016, 38: 629-635
  31. Cadirci E, Oral A, Odabasoglu F, Kilic C, Coskun K, Halici Z, Suleyman H, Nuri Keles O, Unal B. Atorvastatin reduces tissue damage in rat ovaries subjected to torsion and detorsion: biochemical and histopathologic evaluation. NaunynSchmiedeberg's archives of pharmacology, 2010, 381: 455-466.
  32. Yurtcu E, Togrul C, Ozyer S, Uzunlar O, Karatas YH, Seckin KD, Caydere M, Hucumenoglu S, Cicek N. Dose dependent protective effects of vardenafi l on ischemia-reperfusion injury with biochemical and histopathologic evaluation in rat ovary. J Pediatr Surg, 2015, 50: 1205-1209
  33. Yildiz F., CobanS., Terzi A., Ates M, Aksoy N., Cakir H., Ocak A.R., and Bitiren M. Nigella sativa relieves the deleterious effects of ischemia reperfusion injury on liver. World Journal Gastroenterol. 2008, 14(33): 5204–5209.
  34. Curtis CD, Thorngren DL, Nardulli AM. Immunohistochemical analysis of oxidative stress and DNA repair proteins in normal mammary and breast cancer tissues. BMC cancer, 2010, 10:9