Journals / Manas Journal of Agriculture Veterinary and Life Sciences / 2026 / Cilt: 16 Sayı: 1

The Potential Therapeutic Use of Honey, Propolis and Bee Venom in Veterinary Medicine

Veteriner Hekimlik Alanında Bal, Propolis ve Arı Zehirinin Terapötik Amaçlı Kullanım Potansiyelleri

Ceyda Gökçe Vurgun(ARDAHAN ÜNİVERSİTESİ, KAFKAS ARI VE ARICILIK UYGULAMA VE ARAŞTIRMA MERKEZİ)0009-0008-9552-9279
Pages
171–183
Publication Date
2026-06-23
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, recent findings from increasingly intensive scientific research in the field of animal health were evaluated, and the therapeutic potential and possible mechanisms of action of honey, propolis, and bee venom within the scope of veterinary medicine were examined, along with the most recent developments on the subject.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In many countries around the world, bee products have long been used in the treatment and prevention of various diseases, and today they continue to play a role in complementary medicine practices in human health.
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Key Findings & Data:These effects are primarily attributed to the presence of enzymatic hydrogen peroxide, phenolic compounds, the bee-derived antimicrobial peptide defensin-1, maillard reaction products, and antibiotic-like peptides in honey, as well as flavonoids, phenolic acids, esters, and terpenoids in propolis.
💡
Conclusion & Impact:In this study, recent findings from increasingly intensive scientific research in the field of animal health were evaluated, and the therapeutic potential and possible mechanisms of action of honey, propolis, and bee venom within the scope of veterinary medicine were examined, along with the most recent developments on the subject.
🔬Design:Derleme
📍Scope:Comprehensive Sample
⚙️Variables:Arı ürünleri · Arı zehri · Bal

Abstract

In many countries around the world, bee products have long been used in the treatment and prevention of various diseases, and today they continue to play a role in complementary medicine practices in human health. The use of bee products such as honey, propolis, pollen, royal jelly, beeswax, bee venom, and other hive derivatives for therapeutic purposes, as well as for the protection and promotion of health, is defined as apitherapy. However, the application of apitherapy in veterinary medicine is considered a relatively recent approach compared to its use in human medicine. Among bee products, honey, propolis, and bee venom are particularly prominent in terms of usage, and research findings have indicated that these products possess antimicrobial, antiparasitic, immunomodulatory, anti-inflammatory, antioxidant, and antiulcer properties. These effects are primarily attributed to the presence of enzymatic hydrogen peroxide, phenolic compounds, the bee-derived antimicrobial peptide defensin-1, maillard reaction products, and antibiotic-like peptides in honey, as well as flavonoids, phenolic acids, esters, and terpenoids in propolis. Bee venom, has been found to exhibit antimicrobial, anti-inflammatory, analgesic, and antipyretic effects through compounds such as melittin, adolapin, and the enzyme phospholipase A₂. In this study, recent findings from increasingly intensive scientific research in the field of animal health were evaluated, and the therapeutic potential and possible mechanisms of action of honey, propolis, and bee venom within the scope of veterinary medicine were examined, along with the most recent developments on the subject.

Özet

Dünyanın birçok ülkesinde arı ürünleri uzun yıllardır çeşitli hastalıkların tedavisinde ve önlenmesinde kullanılmakta olup günümüzde de pek çok ülkede insan sağlığı alanında tamamlayıcı tıp uygulamalarında yer almaktadır. Bal, propolis, arı zehiri, polen, arı sütü, balmumu ve diğer arı ürünlerinin tedaviye, sağlığın korunmasına ve geliştirilmesine yönelik kullanımını ifade eden apiterapinin Veteriner hekimlikte uygulanması ise, beşeri hekimliğe kıyasla daha yeni bir yaklaşım olarak değerlendirilmektedir. Arı ürünleri içerisinde özellikle bal, propolis, arı zehiri kullanım yaygınlığı açısından ön plana çıkmakta olup, araştırma sonuçları bu ürünlerin antimikrobiyal, antiparaziter, immünomodülatör, antiinflamatuar, antioksidan veya antiülseratif özelliklere sahip olduğunu ortaya koymaktadır. Bu etkiler balda bulunan enzimatik hidrojen peroksit, fenolik bileşikler, arı antimikrobiyal peptid defensin-1'in varlığı, maillard reaksiyonu ürünleri, antibiyotik benzeri peptitler ile propoliste mevcut olan flavonoidler, fenolik asitler, esterler ve terpenoidler gibi biyoaktif bileşikler yoluyla ortaya çıkmaktadır. Arı zehri ise, içerdiği mellitin, adolaptin ve enzim fosfolipaz A2 gibi bileşikler vasıtasıyla antimikrobiyal, antiinflamatuar, analjezik ve antipiretik etkilere sahip olduğu belirlenmiştir. Bu çalışmada, son yıllarda hayvan sağlığı alanında gittikçe yoğunlaşan bilimsel araştırmalardan elde edilen güncel bulgular değerlendirilerek, veteriner hekimliği kapsamında bal, propolis ve arı zehrinin terapötik amaçlı kullanım potansiyelleri ile muhtemel etki mekanizmaları incelenmiş ve konu ile ilgili en son gelişmeler değerlendirilmiştir.

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Cite This Article

Vurgun, C. G. (2026). The Potential Therapeutic Use of Honey, Propolis and Bee Venom in Veterinary Medicine. Manas Journal of Agriculture Veterinary and Life Sciences, Cilt: 16 Sayı: 1, 171–183. https://doi.org/10.53518/mjavl.1869590

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References (101)

  1. Abd El-Wahed, A. A., Khalifa, S. A. M., Sheikh, B. Y., Farag, M. A., Saeed, A., Larik, F. A., Koca-Caliskan, U., Alajlani, M. M., Alharbi, M. G., Atanasov, A. G., & Xiao, J. (2022). Bee venom composition: From chemistry to biological activity. Toxicon, 204, 48–63.
  2. Al-Ani, I. A.-A., Zimmermann, S., Reichling, J., & Wink, M. (2015). Pharmacological synergism of bee venom and melittin with antibiotics and plant secondary metabolites against multi-drug resistant microbial pathogens. Phytomedicine, 22, 245–255.
  3. AlGabbani, Q., Mansour, L., Elnakady, Y. A., Al-Quraishy, S., Alomar, S., Al-Shaebi, E. M., & Abdel-Baki, A. A. (2017). In vivo assessment of the antimalarial and spleen-protective activities of the Saudi propolis methanolic extract. Parasitology Research, 116, 539–547.
  4. Anjum, S. I., Ullah, A., Khan, K. A., Attaullah, M., Khan, H., Ali, H., Bashir, M.A., Tahir, M., Ansari, M.J., Ghramh, H. A., Adgaba, N. & Dash, C. K. (2019). Composition and functional properties of propolis (bee glue): A review. Saudi Journal of Biological Sciences, 26, 1695–1703.
  5. Azam, M. N. K., Ahmed, M. N., Biswas, S., Ara, N., Rahman, M. M., Hirashima, A., & Hasan, M. N. (2018). A review on bioactivities of honey bee venom. Annual Research & Review in Biology, 30, 1–13.
  6. Azman, A. N. S. S., Tan, J. J., Abdullah, M. N. H., Bahari, H., Lim, V., & Yong, Y. K. (2024). Medicinal activities of Tualang honey: A systematic review. BMC Complementary Medicine and Therapies, 24, 358.
  7. Bankova, V., Popova, M., & Trusheva, B. (2014). Propolis volatile compounds. Chemistry Central Journal, 8, 28.
  8. Bava, R., Castagna, F., Musella, V., Lupia, C., Palma, E., & Britti, D. (2023). Therapeutic use of bee venom and potential applications in veterinary medicine. Veterinary Sciences, 10(2), 119.
  9. Bellik, Y. (2015). Bee venom: Its potential use in alternative medicine. Anti-Infective Agents, 13, 3–16.
  10. Berretta, A. A., de Castro, P. A., Cavalheiro, A. H., Fortes, V. S., Bom, V. P., Nascimento, A. P., & Goldman, G. H. (2013). Evaluation of mucoadhesive gels with propolis (EPP-AF) in preclinical treatment of vulvovaginal candidiasis. Evidence-Based Complementary and Alternative Medicine, 641480.
  11. Bischofberger, A. S., Dart, C. M., Horadagoda, N., Perkins, N. R., Jeffcott, L. B., Little, C. B., & Dart, A. J. (2016). Effect of Manuka honey gel on transforming growth factor β1 and β3 concentrations, bacterial counts, and histomorphology of contaminated full-thickness skin wounds in equine distal limbs. Australian Veterinary Journal, 94(1–2), 27–34.
  12. Bistué-Rovira, À., Solé, M., Anguera-Tejedor, M., Pérez, B., García-Tercero, L., Díaz-Pérez, A., Martínez-Benitez, Z., Delgado-Hernández, R., & Jiménez-Altayó, F. (2025). Bee venom disrupts vascular homeostasis: Apitoxin and melittin trigger vascular cell toxicity and aortic dysfunction in mice. Toxicological Sciences, 207(1), 196–207.
  13. Blair, S. E., Cokcetin, N. N., Harry, E. J., & Carter, D. A. (2009). The unusual antibacterial activity of medical-grade Leptospermum honey: Antibacterial spectrum, resistance and transcriptome analysis. European Journal of Clinical Microbiology & Infectious Diseases, 28, 1199–1208.
  14. Boeing, T., da Silva, L. M., Mariott, M., Andrade, S. F., & de Souza, P. (2023). Gastroprotective and ulcer healing effects of Brazilian red propolis in experimental gastric ulcer models. Chemistry & Biodiversity, 20, e202200786.
  15. Bonfim, A. P., Sakita, K. M., Faria, D. R., Arita, G. S., Vendramini, F. A. V. R., Capoci, I. R. G., Braga, A. G., dos Santos, R. S., Bruschi, M. L., Becker, T. C. A., de Oliveira Junior, A. G., Kioshima, É. S., Bonfim-Mendonça, P. d. S., & Svidzinski, T. I. E. (2020). Preclinical approaches in vulvovaginal candidiasis treatment with mucoadhesive thermoresponsive systems containing propolis. PLoS ONE, 15, e0243197.
  16. Bryan, L. E. (1982). Bacterial resistance and susceptibility to chemotherapeutic agents. Cambridge University Press. Cambridge, UK.
  17. Budak, Ö., & Çakıroğlu, H. (2022). Examination the effects of chestnut and Manuka honey for wound healing on mice experimental model. Medical Science and Discovery, 9(6), 170–174.
  18. Carnwath, R., Graham, E. M., Reynolds, K., & Pollock, P. J. (2014). The antimicrobial activity of honey against common equine wound bacterial isolates. The Veterinary Journal, 199, 110–114.
  19. Carpena, M., Nuñez-Estevez, B., Soria-Lopez, A., & Simal-Gandara, J. (2020). Bee venom: An updating review of its bioactive molecules and its health applications. Nutrients, 12(11), 3360.
  20. Cecere, B. G. O., da Silva, A. S., Molosse, V. L., Alba, D. F., Leal, K. W., da Rosa, G., Pereira, W. A. B., da Silva, A. D., Schetinger, M. R. C., Kempka, A. P., Nunes, A., Maraschin, M., Araújo, D. N., Deolindo, G. L., & Vedovatto, M. (2021). Addition of propolis to milk improves lactating lamb’s growth: Effect on antimicrobial, antioxidant and immune responses in animals. Small Ruminant Research, 194, 106265
  21. Chaillou, L. L., & Nazareno, M. A. (2009). Bioactivity of propolis from Santiago del Estero, Argentina, related to their chemical composition. LWT – Food Science and Technology, 42, 1422–1427.
  22. Chatzimisios, K., Tsioli, V., Brellou, G. D., Apostolopoulou, E. P., Angelou, V., Pratsinakis, E. D., Cremers, N. A. J., & Papazoglou, L. G. (2023). Evaluation of the effectiveness of medical-grade honey and Hypericum perforatum ointment on second-intention healing of full-thickness skin wounds in cats. Animals, 14, 36.
  23. Cushnie, T. T., & Lamb, A. J. (2005). Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26, 343–356.
  24. De Barros, P. P., Rossoni, R. D., Garcia, M. T., Kaminski, V. L., Loures, F. V., Fuchs, B. B., Mylonakis, E., & Junqueira, J. C. (2021). The anti-biofilm efficacy of caffeic acid phenethyl ester (CAPE) in vitro and a murine model of oral candidiasis. Frontiers in Cellular and Infection Microbiology, 11, 700305.
  25. De Mendonça, M. A. A., Ribeiro, A. R. S., de Lima, A. K., Bezerra, G. B., Pinheiro, M. S., Albuquerque-Júnior, R. L. C., & Cardoso, J. C. (2020). Red propolis and its regulator formononetin: Evaluation of antioxidant activity and gastroprotective effects in rat model of gastric ulcer. Nutrients, 12(10), 2951.
  26. Değirmencioğlu, H. T. (2018). Evaluation of phenolic profile and activities of Turkish propolis (Yüksek lisans tezi). Yeditepe University. İstanbul, Türkiye.
  27. Fennell, J. F., Shipman, W. H., & Cole, L. J. (1968). Antibacterial action of melittin, a polypeptide from bee venom. Proceedings of the Society for Experimental Biology and Medicine, 127, 707–710.
  28. Fernandes Junior, A., Balestrin, E. C., Betoni, J. E. C., Orsi, R., de Cunha, M., & Montelli, A. C. (2005). Propolis and synergism with antimicrobial drugs. Memórias do Instituto Oswaldo Cruz, 100, 563–566.
  29. Ferreira, L. N., Fonseca, F. P., de Castro, C. C., Figueiredo, M. B., Hubner, S. O., & Camargo, M. M. (2012). Effect of the ethanolic extract from green propolis on production of antibodies after immunization against canine parvovirus and canine coronavirus. Brazilian Journal of Veterinary Research and Animal Science, 49, 116–122.
  30. Gajski, G., Leonova, E., & Sjakste, N. (2024). Bee venom: Composition and anticancer properties. Toxins, 16(3), 117.
  31. Genç, F. & Dodoloğlu, A. (2017). Arıcılığın Temel Esasları. Atatürk Üniversite Ziraat Fakültesi Ofset Tesisi, Erzurum.
  32. Gokulakrishnaa, R., & Thirunavukkarasu, S. (2020). Apitherapy: A valuable gift from honey bee. Journal of Entomology and Zoology Studies, 8, 2317–2323.
  33. Guo, D. S., & Ding, S. L. (2010). Effect of basal diet supplemented with propolis on production performance of weaned piglets. Animal Husbandry and Feed Science, 9, 18–19.
  34. Han, S. M., Lee, G. G., & Park, K. K. (2012). Acute dermal toxicity study of bee venom (Apis mellifera L.) in rats. Toxicological Research, 28(2), 99–102.
  35. Han, S., Yeo, J., Baek, H., Lin, S.-M., Meyer, S., & Molan, P. C. (2009). Postantibiotic effect of purified melittin from honeybee (Apis mellifera) venom against Escherichia coli and Staphylococcus aureus. Journal of Asian Natural Products Research, 11, 796–804.
  36. Harakeh, S., Saber, S. H., Akefe, I. O., Shaker, S., Barkaat Hussain, M., Saad Almasaudi, A., Saleh, S. M. M., & Almasaudi, S. (2022). Saudi honey alleviates indomethacin-induced gastric ulcer via improving antioxidant and anti-inflammatory responses in male albino rats. Saudi Journal of Biological Sciences, 29, 3040–3050.
  37. İnanç, B. (2020). The quality specialities in Türkiye’s honeys for apitherapy. Journal of Pharmacopuncture, 23, 194–200.
  38. Jenkins, R. E., & Cooper, R. (2012). Improving antibiotic activity against wound pathogens with Manuka honey in vitro. PLoS ONE, 7, e45600.
  39. Kabiloğlu, A., Kocabağlı, N., & Kekeç, A. İ. (2023). Effects of propolis extract on growth performance and health condition of dairy calves. Tropical Animal Health and Production, 55, 115.
  40. Kafle, L., & Joshi, R. C. (2023). Apitherapy: An overview. Journal of Applied Zoology Research, 34, 1–7.
  41. Kalkir, F., & Simsek, O. (2026). Mechanisms of Action and Resistance of Antibiotics. Istanus Journal on Applied Biological Sciences, 2(1), e1.
  42. Kan, F. (2025). Biochemical Impacts of Bee Pollen: An Overview. Istanus Journal on Applied Biological Sciences, 1(1), e4.
  43. Khalil, A., Elesawy, B. H., Ali, T. M., & Ahmed, O. M. (2021). Bee Venom: From Venom to Drug. Molecules, 26(16), 4941.
  44. Khalil, M. L. (2006). Biological activity of bee propolis. Asian Pacific Journal of Cancer Prevention, 7, 22–31.
  45. Kim, S.-H., Jun, H.-K., Kim, S., You, M.-J., Jun, M.-H., & Kim, D.-H. (2008). Therapeutic effect of injection- acupuncture with bee-venom (apitoxin) in cases of canine otitis externa. Journal of Veterinary Clinics, 25, 159–164.
  46. Kupczynski, R., Adamski, M., Falta, D., & Roman, A. (2012). The efficiency of propolis in post-costral dairy calves. Archives Animal Breeding, 55, 315–324.
  47. Kwakman, P. H. S., & Zaat, S. A. J. (2012). Antibacterial components of honey. IUBMB Life, 64, 48–55.
  48. Kwakman, P. H. S., Velde, A. A. T., de Boer, L., Speijer, D., Vandenbroucke-Grauls, C. M. J. E. & Zaat, S. A. J. (2010). How honey kills bacteria. FASEB Journal, 24, 2576–2582.
  49. Lee, J., Kim, Y.M., Kim, J.H., Cho, C.W., Jeon, J.W., Park, J.K., Lee, S.H., Jung, B.G., & Lee, B.J. (2018). Nasal delivery of chitosan/alginate nanoparticle-encapsulated bee (Apis mellifera) venom promotes antibody production and viral clearance during porcine reproductive and respiratory syndrome virus infection by modulating T cell- related responses. Veterinary Immunology and Immunopathology, 200, 40–51.
  50. Lee, S. B. (2016). Antifungal activity of bee venom and sweet bee venom against clinically isolated Candida albicans. Journal of Pharmacopuncture, 19, 45-50.
  51. Lima, W. G., Brito, J. C. M., & da Cruz Nizer, W. S. (2024). Therapeutic applications and pharmacological mechanisms of bee venom components. Journal of Venomous Animals and Toxins Including Tropical Diseases, 30, e20230045.
  52. Liu, S., Wang, D., Cao, Y., Lu, T., Liu, H. & Li, S. (2020). Effects of propolis on the immune enhancement of the formalin-inactivated Aeromonas salmonicida vaccine. Aquaculture Research, 51, 4759–4770.
  53. Lukanc, B., Potokar, T., & Erjavec, V. (2018). Observational study of the effect of L-Mesitran® medical honey on wound healing in cats. Veterinarski Arhiv, 88(1), 59–74.
  54. Lukanc, B., Potokar, T., & Erjavec, V. (2020). Complete skin regeneration with medical honey after skin loss on the entire circumference of a leg in a cat. Journal of Tissue Viability, 29(2), 148–152.
  55. Lukanc, B., Šteh, T., & Erjavec, V. (2023). The effect of medical honey on second intention wound healing in dogs. Veterinarski Arhiv, 93(5), 569–580.
  56. Mandal, M. D., & Mandal, S. (2011). Honey: Its medicinal property and antibacterial activity. Asian Pacific Journal of Tropical Biomedicine, 1, 154–160.
  57. Mary, A. (2023). Antibiotics and health hazards of honey. Journal of Apitherapy, 10, 1–2.
  58. Mavric, E., Wittmann, S., Barth, G., & Henle, T. (2008). Identification and quantification of methylglyoxal as the dominant antibacterial constituent of Manuka (Leptospermum scoparium) honeys from New Zealand. Molecular Nutrition & Food Research, 52, 483–489.
  59. Memariani, H., Memariani, M., Moravvej, H., & Shahidi-Dadras, M. (2020). Melittin: A venom-derived peptide with promising anti-viral properties. European Journal of Clinical Microbiology & Infectious Diseases, 39, 5–17.
  60. Montenegro, G., & Mejías, E. (2013). Biological applications of honeys produced by Apis mellifera. Biological Research, 46, 341–345.
  61. Morsy, A. S., Soltan, Y. A., Sallam, S. M. A., & Alencar, S. M. (2016). Impact of Brazilian red propolis extract on blood metabolites, milk production, and lamb performance of Santa Ines ewes. Tropical Animal Health and Production, 48, 1043–1050.
  62. Niu, Y., Wang, K., Zheng, S., Wang, Y., Ren, Q., Li, H., Ding, L., Li, W., & Zhang, L. (2020). Antibacterial effect of caffeic acid phenethyl ester on cariogenic bacteria and Streptococcus mutans biofilms. Antimicrobial Agents and Chemotherapy, 64, e00251-20.
  63. Nolan, V. C., Harrison, J., & Cox, J. A. G. (2019). Dissecting the antimicrobial composition of honey. Antibiotics, 8, 251.
  64. Nweze, N. E., Okoro, H. O., Al Robaian, M., Omar, R. M., Tor-Anyiin, T. A., Watson, D. G., & Igoli, J. O. (2017). Effects of Nigerian red propolis in rats infected with Candida albicans. Comparative Clinical Pathology, 26, 1129–1133.
  65. Ogwu, M. C., & Izah, S. C. (2025). Honey as a natural antimicrobial. Antibiotics, 14(3), 255.
  66. Okinczyc, P., Paluch, E., Franiczek, R., Widelski, J., Wojtanowski, K. K., Mroczek, T., Krzyzanowska, B., Skalicka- Wozniak, K., & Sroka, Z. (2020). Antimicrobial activity of Apis mellifera L. and Trigona sp. propolis from Nepal and its phytochemical analysis. Biomedicine & Pharmacotherapy, 129, 110435.
  67. Orsolic, N., & Jazvinscak Jembrek, M. (2022). Molecular and cellular mechanisms of propolis and its polyphenolic compounds against cancer. International Journal of Molecular Sciences, 23, 10479.
  68. Oryan, A., Alemzadeh, E., & Moshiri, A. (2016). Biological properties and therapeutic activities of honey in wound healing: A narrative review and meta-analysis. Journal of Tissue Viability, 25, 98–118.
  69. Özdemir, V. F., & Yanar, M. (2024). Effects of propolis extract administration on immune parameters, faecal consistency scores, and growth performance of Holstein–Friesian calves. Tropical Animal Health and Production, 56, 259.
  70. Özdemir, V., Yanar, M., & Koçyiğit, R. (2022). General properties of propolis and its usage in ruminants. Journal of the Hellenic Veterinary Medical Society, 73, 3905–3912.
  71. Park, J. H., Kum, Y. S., Bae, S., Park, S. S., Kim, Y. S., & Kim, H. W. (2021). Immunomodulatory effects of phospholipase A2 from bee venom in inflammatory diseases. Toxins, 13(3), 206.
  72. Pasupuleti, V. R., Sammugam, L., Ramesh, N., & Gan, S. H. (2017). Honey, propolis, and royal jelly: A comprehensive review of their biological actions and health benefits. Oxidative Medicine and Cellular Longevity, 1259510.
  73. Perumal Samy, R., Gopalakrishnakone, P., Thwin, M. M., Chow, T. K. V., Bow, H., Yap, E. H., & Thong, T. W. J. (2007). Antibacterial activity of snake, scorpion and bee venoms: A comparison with purified venom phospholipase A2 enzymes. Journal of Applied Microbiology, 102, 650–659.
  74. Peteoaca, A., Istrate, A., Goanta, A., Girdan, G., Stefanescu, A., & Tanase, A. (2019). Therapeutic approach in managing degloving injuries of the front limbs in a dog: A case report. Scientific Works. Series C. Veterinary Medicine, 65, 70–78.
  75. Peter, C. M., Picoli, T., Zani, J. L., Latosinski, G. S., Lima, M., Vargas, G. D., Hübner, S. O., & Fischer, G. (2017). Antiviral and virucidal activity of hydroalcoholic extracts of propolis brown, green and jataí bees (Tetragonisca angustula) against bovine herpesvirus type-1 (BoHV-1) and bovine viral diarrhea virus (BVDV). Pesquisa Veterinária Brasileira, 37, 667–675.
  76. Przybylek, I., & Karpiński, T. M. (2019). Antibacterial properties of propolis. Molecules, 24(11), 2047.
  77. Rozik, O. I., Hussein, M. M., El-Elebiarie, A. S., & Nady, S. (2024). In vitro anti-inflammatory activity of bee venom melittin and phospholipase A₂ on murine splenocytes stimulated with Schistosoma mansoni antigens. Egyptian Academic Journal of Biological Sciences, C: Physiology & Molecular Biology, 16(2), 75–88.
  78. Ruiz-Hurtado, P. A., Garduño-Siciliano, L., Domínguez-Verano, P., Balderas-Cordero, D., Gorgua-Jiménez, G., & Rodríguez-Monroy, M. A. (2021). Propolis and its gastroprotective effects on NSAID-induced gastric ulcer disease: A systematic review. Nutrients, 13(9), 3169
  79. Salmela, H., Amdam, G. V., & Freitak, D. (2015). Vitellogenin is an antimicrobial acute phase protein in honey bees. Scientific Reports, 5, 17093.
  80. Santos, L. A., Rosalen, P. L., Dias, N. A., Grisolia, J. C., Gomes, B. J. N., Blosfeld-Lopes, L., Ikegaki, M., Alencar, S. M., & Burger, E. (2021). Brazilian red propolis shows antifungal and immunomodulatory activities against Paracoccidioides brasiliensis. Journal of Ethnopharmacology, 277, 114181.
  81. Saranraj, P., & Sivasakthi, S. (2018). Comprehensive review on honey: Biochemical and medicinal properties. Journal of Academic and Industrial Research, 6, 165–181.
  82. Sarker, M. S. K., & Yang, C. J. (2010). Propolis and illite as feed additives on performance and blood profiles of pre-weaning Hanwoo calves. Journal of Animal and Veterinary Advances, 9, 2526–2531.
  83. Sforcin, J., Fernandes, A., Lopes, C., Bankova, V., & Funari, S. (2000). Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacology, 73, 243–249.
  84. Shedeed, H. A., Farrag, B., Elwakeel, E. A., El-Hamid, I. S. A., & El-Rayes, M. A. H. (2019). Propolis supplementation improved productivity, oxidative status, and immune response of Barki ewes and lambs. Veterinary World, 12, 834–843.
  85. Silva, J. F. M., de Souza, M. C., Matta, S. R., de Andrade, M. R., & Vidal, F. V. N. (2006). Correlation analysis between phenolic levels of Brazilian propolis extracts and their antimicrobial and antioxidant activities. Food Chemistry, 99, 431–435.
  86. Silva, M. P., Silva, T. M., Mengarda, A. C., Salvadori, M. C., Teixeira, F. S., Alencar, S. M., Luz Filho, G. C., Bueno‑Silva, B., & de Moraes, J. (2021). Brazilian red propolis exhibits antiparasitic properties in vitro and reduces worm burden and egg production in a mouse model harboring either early or chronic Schistosoma mansoni infection. Journal of Ethnopharmacology, 264, 113387.
  87. Silva-Carvalho, R., Baltazar, F., & Almeida-Aguiar, C. (2015). Propolis: A complex natural product with a plethora of biological activities that can be explored for drug development. Evidence-Based Complementary and Alternative Medicine, 2015, 206439.
  88. Slanzo, G., Toledo, A. F., Silva, A. P., Coelho, M. G., Silva, M. D., Cezar, A. M., & Bittar, C. M. M. (2019). Red propolis as an additive for preweaned dairy calves: Effect on growth performance, health, and selected blood parameters. Journal of Dairy Science, 102, 8952–8962.
  89. Socarras, K. M., Theophilus, P. A. S., Torres, J. P., Gupta, K., & Sapi, E. (2017). Antimicrobial activity of bee venom and melittin against Borrelia burgdorferi. Antibiotics, 6, 31.
  90. Stevanovic, J., Glavinić, U., Ristanić, M., Erjavec, V., Denk, B., Dolašević, S., & Stanimirović, Z. (2024). Bee‑inspired healing: Apitherapy in veterinary medicine for maintenance and improvement animal health and well‑being. Pharmaceuticals, 17, 1050.
  91. Sung, S. H., Choi, G. H., Lee, N. W., & Shin, B. C. (2017). External use of propolis for oral, skin, and genital diseases: A systematic review and meta‑analysis. Evidence-Based Complementary and Alternative Medicine, 8025752.
  92. Şahin, H., Kaltalıoğlu, K., Erişgin, Z., Coşkun-Cevher, Ş., & Kolaylı, S. (2023). Comparison of protective effect of aqueous and ethanolic extracts of propolis against gastric ulceration in rats. Chemistry & Biodiversity, 20(5).
  93. Uddin, M. B., Lee, B.‑H., Nikapitiya, C., Kim, J.‑H., Kim, T.‑H., Lee, H.‑C., Kim, C. G., Lee, J.‑S., & Kim, C. J. (2016). Inhibitory effects of bee venom and its components against viruses in vitro and in vivo. Journal of Microbiology, 54, 853–866.
  94. Velazquez, C., Navarro, M., Acosta, A., Angulo, A., Dominguez, Z., Robles, R., Robles‑Zepeda, R., Lugo, E., Goycoolea, F. M., Velazquez, E. F., Astiazaran, H., & Hernandez, J. (2007). Antibacterial and free‑radical scavenging activities of Sonoran propolis. Journal of Applied Microbiology, 103, 1747–1756.
  95. Vogt, N. A., Vriezen, E., Nwosu, A., & Sargeant, J. M. (2021). A scoping review of the evidence for the medicinal use of natural honey in animals. Frontiers in Veterinary Science, 7, 618301.
  96. Wagh, V. D. (2013). Propolis: A wonder bees product and its pharmacological potentials. Advances in Pharmacological and Pharmaceutical Sciences, 308249.
  97. Yoo, J., & Lee, G. (2022). Adverse Events Associated with the Clinical Use of Bee Venom: A Review. Toxins, 14(8), 562.
  98. Yücel, B., Önenç, A., Kaya, A., & Altan, Ö. (2015). Effects of propolis administration on growth performance and neonatal diarrhea of calves. SOJ Veterinary Sciences, 1, 102–106.
  99. Bryan, L. E. (1982). Bacterial resistance and susceptibility to chemotherapeutic agents. Cambridge University Press. Cambridge, UK.
  100. Chaillou, L. L., & Nazareno, M. A. (2009). Bioactivity of propolis from Santiago del Estero, Argentina, related to their chemical composition. LWT – Food Science and Technology, 42, 1422–1427.
  101. Santos, L. A., Rosalen, P. L., Dias, N. A., Grisolia, J. C., Gomes, B. J. N., Blosfeld-Lopes, L., Ikegaki, M., Alencar, S. M., & Burger, E. (2021). Brazilian red propolis shows antifungal and immunomodulatory activities against Paracoccidioides brasiliensis. Journal of Ethnopharmacology, 277, 114181.