SavedAdd your journal

Journals / Journal of Agriculture / 2026 / Cilt: 9 Sayı: 1

Effects of Berberine and Curcumin on Some Oxidative Stress Parameters of the Bladder in Cyclophosphamide-Induced Interstitial Cystitis

Siklofosfamid Kaynaklı İnterstisyel Sistitte Berberin ve Kurkuminin Mesane Üzerine Bazı Oksidatif Stres Parametrelerin Etkileri

Ugur Ozdek(VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, VAN SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU)0000-0002-0709-1545
Sinan Mean(VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ)0009-0004-8823-7527
Pages
—
Publication Date
2026-07-08
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, the biochemical data revealed that berberine (BER) and curcumin (KUR) administration significantly suppressed lipid peroxidation in cyclophosphamide (CYP) structural injury and repaired endogenous antioxidant defense mechanisms.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study demonstrates the modulation of oxidative stress levels in traumatic conditions by cyclophosphamide (CYP) and berberine (BER) and curcumin (KUR), known for their potent antioxidant properties.
📊
Key Findings & Data:A total of 36 female Wistar-Albino mice were randomly divided into six groups (n=6): Control, DMSO, CYP (75 mg/kg), CYP+BER (50 mg/kg), CYP+KUR (50 mg/kg), and CYP+BER+KUR.
💡
Conclusion & Impact:In conclusion, the biochemical data revealed that berberine (BER) and curcumin (KUR) administration significantly suppressed lipid peroxidation in cyclophosphamide (CYP) structural injury and repaired endogenous antioxidant defense mechanisms.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Siklofosfamid · Berberin · Kurkumin

Abstract

This study demonstrates the modulation of oxidative stress levels in traumatic conditions by cyclophosphamide (CYP) and berberine (BER) and curcumin (KUR), known for their potent antioxidant properties. A total of 36 female Wistar-Albino mice were randomly divided into six groups (n=6): Control, DMSO, CYP (75 mg/kg), CYP+BER (50 mg/kg), CYP+KUR (50 mg/kg), and CYP+BER+KUR. Oxidative stress levels were analyzed by measuring malondialdehyde (MDA) levels and catalase (CAT) and glutathione (GSH) activities. Cyclophosphamide (CYP) administration resulted in a significant increase in MDA levels, a marker of lipid peroxidation, in bladder tissue. BER and KUR treatments significantly suppressed MDA levels, and enzyme activity remained under control for a normal duration. Synergistic improvement, particularly observed in the combined (BER+KUR) groups, was more pronounced compared to single treatments. In conclusion, the biochemical data revealed that berberine (BER) and curcumin (KUR) administration significantly suppressed lipid peroxidation in cyclophosphamide (CYP) structural injury and repaired endogenous antioxidant defense mechanisms. These findings suggest that these phytochemicals may play a role in protecting the body by limiting oxidative infection and could be considered promising therapeutic agents for the management of interstitial cystitis.

Özet

Bu çalışma, siklofosfamid (CYP) kaynaklı mesane hasarında gelişen oksidatif stres yanıtının, güçlü antioksidan özellikleri ile bilinen berberin (BER) ve kurkumin (KUR) ile modüle edilebilirliğini araştırmayı amaçlamaktadır. Çalışmada toplam 36 dişi Wistar-Albino sıçan rastgele altı gruba ayrıldı (n=6): Kontrol, DMSO, CYP (75 mg/kg), CYP+BER (50 mg/kg), CYP+KUR (50 mg/kg) ve CYP+BER+KUR. Oksidatif stres düzeyleri; malondialdehit (MDA) seviyeleri ile katalaz (CAT) ve glutatyon (GSH) aktiviteleri ölçülerek analiz edildi. Siklofosfamid (CYP) uygulaması, mesane dokusunda lipid peroksidasyon belirteci olan MDA düzeylerinde anlamlı bir artış ile sonuçlanmıştır. BER ve KUR tedavileri, MDA düzeyinde anlamlı bir şekilde baskılamış ve antioksidan enzim aktivitelerinin kontrol seviyelerini normal düzeye getirmiştir. Özellikle kombine (BER+KUR) grubunda gözlenen sinerjistik iyileşme, tekli tedavilere göre daha belirgin bulunmuştur. Sonuç olarak, elde edilen biyokimyasal veriler; berberin (BER) ve kurkumin (KUR) uygulamalarının, siklofosfamid (CYP) kaynaklı mesane hasarında lipid peroksidasyonun belirgin şekilde baskı ettiğini ve endojen antioksidan savunma mekanizmalarını restore ettiğini ortaya koymaktadır. Bu bulgular, söz konusu fitokimyasalların oksidatif hasarı sınırlayarak mesane dokusunu korumada stratejik bir rol oynayabileceğine ve interstisyel sistit yönetimi için umut verici terapötik ajanlar olarak değerlendirilebileceğine işaret etmektedir.

🔖

Cite This Article

Ozdek, U., Mean, S. (2026). Effects of Berberine and Curcumin on Some Oxidative Stress Parameters of the Bladder in Cyclophosphamide-Induced Interstitial Cystitis. Journal of Agriculture, Cilt: 9 Sayı: 1. https://doi.org/10.46876/ja.1911388

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (26)

  1. Akbaş, N., Suleyman, B., Mammadov, R., Yazıcı, G.N., Bulut, S., Süleyman, H., 2022. Effect of taxifolin on cyclophosphamide-induced oxidative and inflammatory bladder injury in rats. Experimental Animals, 71(4), 460–467.
  2. Alagawany, M., Farag, M.R., Abdelnour, S.A., Dawood, M.A.O., Elnesr, S.S., Dhama, K., 2021. Curcumin and its different forms: a review on fish nutrition. Aquaculture, 532, 736030.
  3. Alizadeh, M., Kheirouri, S., 2019. Curcumin reduces malondialdehyde and improves antioxidants in humans with diseased conditions: A comprehensive meta-analysis of randomized controlled trials. Biomedicine (Taipei), 9(4), 23.
  4. Aslankoç, R., Demirci, D., İnan, Ü., Yıldız, M., Öztürk, A., Çetin, M., Savran, E.Ş., Yılmaz, B., 2019. The role of antioxidant enzymes in oxidative stress: Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi, 26(3), 362–369.
  5. Çakına, S., İrkin, L.C., Öztürk, Ş., 2024. Protective effects of curcumin and resveratrol on kidney tissue on cadmium-induced oxidative stress in rats. Gazi Medical Journal, 35(2), 145–148.
  6. Gaweł, S., Wardas, M., Niedworok, E., Wardas, P., 2004. Malondialdehyde (MDA) as a lipid peroxidation marker. Wiadomosci Lekarskie, 57(9-10), 453–455.
  7. Germoush, M.O., Mahmoud, A.M., 2014. Berberine mitigates cyclophosphamide-induced hepatotoxicity by modulating antioxidant status and inflammatory cytokines. Cancer Research and Clinical Oncology, 140(7), 1103–1109.
  8. Gonzalez, E.J., Peterson, A., Malley, S., Daniel, M., Lambert, D., Kosofsky, M., Vizzard, M.A., 2015. The effects of tempol on cyclophosphamide-induced oxidative stress in rat micturition reflexes. Scientific World Journal, 2015, 545048.
  9. Hasanein, P., Ghafari-Vahed, M., Khodadadi, I., 2016. Effects of isoquinoline alkaloid berberine on lipid peroxidation, antioxidant defense system, and liver damage induced by lead acetate in rats. Redox Report, 22, 42–50.
  10. İnce, S., Küçükkurt, İ., Demirel, H.H., Acaroz, D.A., Akbel, E., Cigerci, İ.H., 2014. Protective effects of boron on cyclophosphamide-induced lipid peroxidation and genotoxicity in rats. Chemosphere, 108, 197–204.
  11. Khan, H., Khan, M.F., Jan, S.U., Khan, K.A., Shah, K.U., Badshah, A., 2011. Concentration and time dependent effect of aluminium metal on glutathione level in plasma of human blood. Journal of Applied Pharmacy, 3(3), 279–288.
  12. Lartillot, S., Kedziora, P., Athias, A., 1988. Purification and characterization of a new fungal catalase. Preparative Biochemistry, 18(3), 241–246.
  13. Lin, X., Bai, D., Wei, Z., Zhang, Y., Huang, Y., Deng, H., Huang, X., 2019. Curcumin attenuates oxidative stress in RAW264.7 cells by increasing the activity of antioxidant enzymes and activating the Nrf2-Keap1 pathway. PLoS ONE, 14(5), e0216711.
  14. Luo, J., Yang, C., Luo, X., et al., 2020. Chlorogenic acid attenuates cyclophosphamide-induced rat interstitial cystitis. Life Sciences, 254, 117590.
  15. Mombeini, M.A., Kalantar, H., Sadeghi, E., Goudarzi, M., Khalili, H., Kalantar, M., 2022. Protective effects of berberine as a natural antioxidant and anti-inflammatory agent against nephrotoxicity induced by cyclophosphamide in mice. Naunyn-Schmiedeberg’s Archives of Pharmacology, 395(2), 187–194.
  16. Nandi, A., Yan, L.J., Jana, C.K., Das, N., 2019. Oksidatif stres ve yaşla ilişkili dejeneratif hastalıklarda katalazın rolü. Oxidative Medicine and Cellular Longevity, 2019, 9613090.
  17. Ni, B., Chen, Z., Shu, L., Shao, Y., Huang, Y., Elias Tamrat, N., Wei, Z., Shen, B., 2021. Nrf2 pathway ameliorates bladder dysfunction in cyclophosphamide-induced cystitis via suppression of oxidative stress. Oxidative Medicine and Cellular Longevity, 2021, 4009308.
  18. Özcan, O., Erdal, H., Çakırca, G., Yönden, Z., 2015. Oksidatif stres ve hücre içi lipit, protein ve DNA yapıları üzerine etkileri / Oxidative stress and its impacts on intracellular lipids, proteins and DNA. Journal of Clinical and Experimental Investigations, 6(3), 331–336.
  19. Patel, S.S., Acharya, A., Ray, R.S., Agrawal, R., Raghuwanshi, R., Jain, P., 2019. Cellular and molecular mechanisms of curcumin in prevention and treatment of disease. Critical Reviews in Food Science and Nutrition, 60, 887–939.
  20. Presnell, C.E., Bhatti, G., Numan, L.S., Lerche, M., Alkhateeb, S.K., Ghalib, M., Shammaa, M., Kavdia, M., 2013. Computational insights into the role of glutathione in oxidative stress. Current Neurovascular Research, 10(2), 185–194.
  21. Sherif, I.O., 2018. The effect of natural antioxidants in cyclophosphamide-induced hepatotoxicity: Role of Nrf2/HO-1 pathway. International Immunopharmacology, 61, 29–36.
  22. Sherif, I.O., 2018. Uroprotective mechanism of quercetin against cyclophosphamide-induced urotoxicity: effect on oxidative stress and inflammatory markers. Journal of Cellular Biochemistry, 119, 7441–7448.
  23. Suskind, A.M., Berry, S.H., Ewing, B.A., Elliott, M.N., Suttorp, M.J., Clemens, J.Q., 2013. The prevalence and overlap of interstitial cystitis/bladder pain syndrome and chronic prostatitis/chronic pelvic pain syndrome in men: Results of the RAND interstitial cystitis epidemiology male study. Journal of Urology, 189, 141–145.
  24. Wang, H., Wu, P., Li, F., Shin, J., Ki, J.S., 2024. Molecular characterization of a catalase gene in the freshwater green alga Closterium ehrenbergii and its putative function against abiotic stresses. European Journal of Protistology, 95, 126111.
  25. Wen, C., Xie, L., Hu, C., 2022. Roles of mesenchymal stem cells and exosomes in interstitial cystitis/bladder pain syndrome. Journal of Cellular and Molecular Medicine, 26(3), 624–635.
  26. Xu, W., Fang, C., Jiang, M.J., 2025. The therapeutic effect of berberine on rodent models of kidney injury: A systematic review and meta-analysis. BMC Nephrology, 26, 315.