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Journals / International Journal of Chemistry and Technology / 2019 / Volume: 3 Issue: 2

Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology

Yasemin Bozkurt(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ)0000-0002-3456-3261
Sema Bilgin(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ)0000-0001-5921-5434
Seçil Erden(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ)0000-0001-8473-5896
İsmail Furkan Turan(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ)0000-0002-2391-1582
İsa Gökçe(TOKAT GAZİOSMANPAŞA ÜNİVERSİTESİ)0000-0002-5023-9947
Pages
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Publication Date
2019-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Additionally, it was determined that the migration ability of the HUVECs was promoted by rhG-CSF in a concentration-dependent manner by in vitro scratch assay.

📌 3 Key Takeaways

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Objective & Hypothesis:The human granulocyte colony stimulating factor (hG-CSF) is a member of the CSF family.
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Key Findings & Data:The first purpose of this study was production of recombinant human G-CSF (rhG-CSF) which is an important therapeutic protein for angiogenesis based clinical applicaitons. rhG-CSF was produced as inclusion body in the Escherichia coli strain BL21 (DE3) pLysE with pTOLT vector system.
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Conclusion & Impact:Additionally, it was determined that the migration ability of the HUVECs was promoted by rhG-CSF in a concentration-dependent manner by in vitro scratch assay.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Granulocyte colony stimulating factor · recombinant proteins · angiogenesis

Abstract

The human granulocyte colony stimulating factor (hG-CSF) is a member of the CSF family. The first purpose of this study was production of recombinant human G-CSF (rhG-CSF) which is an important therapeutic protein for angiogenesis based clinical applicaitons. rhG-CSF was produced as inclusion body in the Escherichia coli strain BL21 (DE3) pLysE with pTOLT vector system. The rhG-CSF was purified by Ni-NTA agarose afinity chromatography and characterized with SDS-PAGE analysis. The effects of this therapeutic protein on cell viability was measured by MTT assay. Additionally, angiogenic potential of produced rhG-CSF was investigated via HUVEC cell line by in vitro scratch assay. As a result, the purified protein induced cell proliferation and the EC50 value of protein based drug candidate was 0.051 mM. Additionally, it was determined that the migration ability of the HUVECs was promoted by rhG-CSF in a concentration-dependent manner by in vitro scratch assay.

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Cite This Article

Bozkurt, Y., Bilgin, S., Erden, S., Turan, İ. F., Gökçe, İ. (2019). Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology. International Journal of Chemistry and Technology, Volume: 3 Issue: 2. https://doi.org/10.32571/ijct.600180

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