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Journals / International Journal of Chemistry and Technology / 2025 / Volume: 9 Issue: 2

Evaluation of Systemic Oxidative Damage Following Acute Exposure to the Azo Dye Sudan III in Rats

Ezgi Kar(KUTAHYA HEALTH SCIENCES UNIVERSITY)0000-0003-2134-4067
Yakup Gülekçi(KUTAHYA HEALTH SCIENCES UNIVERSITY)0000-0001-9643-6850
Harun Şener(KUTAHYA HEALTH SCIENCES UNIVERSITY)0000-0003-3521-0684
Gamze Güler(ESKISEHIR OSMANGAZI UNIVERSITY)0000-0003-1164-5448
Hakan Şentürk(ESKISEHIR OSMANGAZI UNIVERSITY)0000-0002-6199-8594
Fatih Kar(KUTAHYA HEALTH SCIENCES UNIVERSITY)0000-0001-8356-9806
Pages
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Publication Date
2025-12-29
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These results demonstrate that even short-term exposure to Sudan III provokes systemic oxidative stress and suppresses the antioxidant defense system.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Our study aimed to assess the acute oxidative stress induced by Sudan III, an azo dye frequently misused as an illegal food additive.
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Key Findings & Data:Twenty-four male Sprague–Dawley rats were randomly allocated into three groups (n = 8 per group): Control, Low Dose (125 mg/kg), and High Dose (250 mg/kg).
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Conclusion & Impact:These results demonstrate that even short-term exposure to Sudan III provokes systemic oxidative stress and suppresses the antioxidant defense system.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Sudan III · oxidative stress · antioxidant defense

Abstract

Our study aimed to assess the acute oxidative stress induced by Sudan III, an azo dye frequently misused as an illegal food additive. Twenty-four male Sprague–Dawley rats were randomly allocated into three groups (n = 8 per group): Control, Low Dose (125 mg/kg), and High Dose (250 mg/kg). A single oral administration was performed, and oxidative stress biomarkers along with antioxidant defense parameters were analyzed 24h post-exposure. Rats receiving the high dose of Sudan III exhibited significant elevations in total oxidant status (TOS), oxidative stress ındex (OSI), reactive oxygen species (ROS), and malondialdehyde (MDA), accompanied by pronounced reductions in total antioxidant status (TAS), native thiol, total thiol, disulfide, superoxide dismutase (SOD), and catalase (CAT). These results demonstrate that even short-term exposure to Sudan III provokes systemic oxidative stress and suppresses the antioxidant defense system. Overall, the findings highlight the health risks linked to the illicit use of Sudan III in food products and reinforce the need for stricter regulatory enforcement to ensure consumer safety.

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Cite This Article

Kar, E., Gülekçi, Y., Şener, H., Güler, G., Şentürk, H., Kar, F. (2025). Evaluation of Systemic Oxidative Damage Following Acute Exposure to the Azo Dye Sudan III in Rats. International Journal of Chemistry and Technology, Volume: 9 Issue: 2. https://doi.org/10.32571/ijct.1781337

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