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Journals / Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi / 2026 / Volume: 46 Issue: 2

Total Phenolic and Flavonoid Contents, Antioxidant and Cytotoxic Activities of Vincetoxicum fuscatum subsp. fuscatum seeds

Didem Aksu(EGE UNIVERSITY)0000-0002-7184-4011
Sevda Güzel Kara(MERSIN UNIVERSITY)0000-0002-6642-5824
Pages
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Publication Date
2026-06-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This is the first study to investigate the total phenolic and total flavonoid contents, as well as the antioxidant and cytotoxic activities of V. fuscatum subsp. fuscatum seeds.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The genus Vincetoxicum Wolf (Apocynaceae: Asclepiadoideae), naturally distributed across Africa, Australia, Asia, and Europe, comprises approximately 140 species.
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Key Findings & Data:Total phenolic and flavonoid contents, along with antioxidant activity, were studied using the Folin-Ciocalteu, AlCl3, and DPPH assays, respectively.
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Conclusion & Impact:This is the first study to investigate the total phenolic and total flavonoid contents, as well as the antioxidant and cytotoxic activities of V. fuscatum subsp. fuscatum seeds.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Vincetoxicum fuscatum subsp. fuscatum · DPPH · HaCaT cell line

Abstract

The genus Vincetoxicum Wolf (Apocynaceae: Asclepiadoideae), naturally distributed across Africa, Australia, Asia, and Europe, comprises approximately 140 species. Species within this genus have been used in traditional medicine to treat wounds, fractures, inflammation, and envenomations from snake, insect, and scorpions. Vincetoxicum is one of the most species-rich genera of the subfamily in Anatolia. Vincetoxicum fuscatum subsp. fuscatum is known as “dağ biberi, gâvur üzerliği, and gâvur biberi” in Turkey. This research focused on analyzing ethanol extract from seeds of V. fuscatum subsp. fuscatum, to assess its phenolic and flavonoid contents, antioxidant activity, and cytotoxic profile. Total phenolic and flavonoid contents, along with antioxidant activity, were studied using the Folin-Ciocalteu, AlCl3, and DPPH assays, respectively. Cytotoxicity was tested on HaCaT cell line using the MTT assay. The total phenolic and flavonoid contents and antioxidant activity were 7.42 ± 0.04 mg GAE/g seed; 3.08 ± 0.01 mg QE/g seed; and 27.3 ± 0.01% inhibition, equivalent to 0.26 ± 0.06 mM AAE/g seed, respectively. All tested concentrations of the extract (0.25-1 mg/mL) did not exhibit any cytotoxic effect on the HaCaT cell line. This is the first study to investigate the total phenolic and total flavonoid contents, as well as the antioxidant and cytotoxic activities of V. fuscatum subsp. fuscatum seeds.

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Cite This Article

Aksu, D., Kara, S. G. (2026). Total Phenolic and Flavonoid Contents, Antioxidant and Cytotoxic Activities of Vincetoxicum fuscatum subsp. fuscatum seeds. Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, Volume: 46 Issue: 2. https://doi.org/10.52794/hujpharm.1870361

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