Journals / Black Sea Journal of Engineering and Science / 2026 / Cilt: 9 Sayı: 1

Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles with Safflower (Carthamus tinctorius L.)

Aspir (Carthamus tinctorius L.) ile Gümüş Nanoparçacıkların Yeşil Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesi

Elif Özbey(MALATYA TURGUT ÖZAL ÜNİVERSİTESİ)0000-0001-7215-1922
Gülçin Beker Akbulut(MALATYA TURGUT ÖZAL ÜNİVERSİTESİ)0000-0003-3529-5999
Gülşah Yıl(MALATYA TURGUT ÖZAL ÜNİVERSİTESİ)0000-0001-5124-7239
Ülkü Yılmaz(MALATYA TURGUT ÖZAL ÜNİVERSİTESİ)0000-0002-2806-4781
Pages
218–225
Publication Date
2026-01-15
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the highest synthesis efficiency and the most regular nanoparticle morphology were demonstrate from the flower extract, producing AgNPs with an average size of approximately 40 nm.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to synthesize silver nanoparticles (AgNPs) using the leaf, flower, and seed extracts of safflower (Carthamus tinctorius L.) and to evaluate their antimicrobial activities.
📊
Key Findings & Data:The results showed that the highest synthesis efficiency and the most regular nanoparticle morphology were demonstrate from the flower extract, producing AgNPs with an average size of approximately 40 nm.
💡
Conclusion & Impact:Overall, it has been concluded that AgNPs derived from cornflowers can be considered as potential antimicrobial agents.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Yeşil sentez · Gümüş nanoparçacıklar (AgNP’ler) · Carthamus tinctorius L. (Aspir)

Abstract

The aim of this study was to synthesize silver nanoparticles (AgNPs) using the leaf, flower, and seed extracts of safflower (Carthamus tinctorius L.) and to evaluate their antimicrobial activities. For this purpose, plant extracts were used as reducing and stabilizing agents, and the synthesized AgNPs were characterized by UV–Vis spectroscopy, SEM-EDX, and FTIR analyses. In addition, the chemical composition of the flower extract was determined by GC-MS. The antimicrobial activity of the AgNPs was tested against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Saccharomyces cerevisiae and Candida albicans using the disk diffusion method. The results showed that the highest synthesis efficiency and the most regular nanoparticle morphology were demonstrate from the flower extract, producing AgNPs with an average size of approximately 40 nm. It has been determined that AgNPs synthesized with flower extract form the widest inhibition zones, particularly in B. subtilis, P. aeruginosa, and E. coli strains, while no inhibition was observed in fungal species. Overall, it has been concluded that AgNPs derived from cornflowers can be considered as potential antimicrobial agents.

Özet

Bu çalışmanın amacı, aspir (Carthamus tinctorius L.) bitkisinin yaprak, çiçek ve tohum ekstraktları kullanılarak gümüş nanoparçacıkların (AgNP) sentezlenmesi ve bu nanoparçacıkların antimikrobiyal aktivitelerinin değerlendirilmesidir. Bu kapsamda bitki ekstraktları indirgeme ve kaplama ajanı olarak kullanılmış; sentezlenen AgNP’ler UV–Vis spektroskopisi, SEM-EDX ve FTIR analizleri ile karakterize edilmiştir. Ayrıca çiçek ekstraktının kimyasal bileşimi GC-MS ile belirlenmiştir. AgNP’lerin antimikrobiyal aktivitesi disk difüzyon yöntemi kullanılarak Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Saccharomyces cerevisiae ve Candida albicans üzerinde test edilmiştir. Bulgular, en yüksek sentez veriminin ve en düzenli nanopartikül morfolojisinin çiçek ekstraktı ile elde edildiğini ve ortalama boyutu 40 nm olan AgNP’lerin oluştuğunu göstermiştir. Çiçek ekstraktıyla sentezlenen AgNP’ lerin özellikle B. subtilis, P. aeruginosa ve E. coli suşlarında en geniş inhibisyon zonlarını oluşturduğu belirlenmiştir, mantar türlerinde ise inhibisyon gözlenmemiştir. Genel olarak, aspir çiçeği kaynaklı AgNP’lerin potansiyel antimikrobiyal ajanlar olarak değerlendirilebileceği sonucuna varılmıştır.

🔖

Cite This Article

Özbey, E., Akbulut, G. B., Yıl, G., Yılmaz, Ü., Asma, D. (2026). Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles with Safflower (Carthamus tinctorius L.). Black Sea Journal of Engineering and Science, Cilt: 9 Sayı: 1, 218–225. https://doi.org/10.34248/bsengineering.1782683

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (36)

  1. Ahmed, S., Ahmad, M., Swami, B. L., & Ikram, S. (2016a). A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. Journal of Advanced Research, 7(1), 17–28. https://doi.org/10.1016/j.jare.2015.02.007
  2. Ahmed, S., Saifullah, Ahmad, M., Swami, B. L., & Ikram, S. (2016b). Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. Journal of Radiation Research and Applied Sciences, 9(1), 1–7. https://doi.org/10.1016/j.jrras.2015.02.007
  3. Aparna, V., Dileep, K. V., Mandal, P. K., Karthe, P., Sadasivan, C., & Haridas, M. (2012). Anti-inflammatory property of n-hexadecanoic acid: Structural evidence and kinetic assessment. Chemical Biology & Drug Design, 80(3), 434–439. https://doi.org/10.1111/j.1747-0285.2012.01324.x
  4. Ashri, A., Zimmer, E., Urie, L., & Ghaner, A. (1974). Evaluation of the world collection of safflower for yield components and their relationship. Crop Science, 14(6), 799–802. https://doi.org/10.2135/cropsci1974.0011183X001400060006x
  5. Babaoğlu, M. (2007). Aspir bitkisi ve tarımı(Bilgi broşürü). Trakya Tarımsal Araştırma Enstitüsü.
  6. Baydar, H. (2016). Tıbbi ve aromatik bitkiler bilimi ve teknolojisi (Güncellenmiş baskı). SDÜ Yayınları.
  7. Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582. https://doi.org/10.1128/CMR.12.4.564
  8. Cushnie, T. P. T.,& Lamb, A. J. (2011). Recent advances in understanding the antibacterial properties of flavonoids. International Journal of Antimicrobial Agents, 38(2), 99–107. https://doi.org/10.1016/j.ijantimicag.2011.02.014
  9. Dajue, L.,& Mündel, H. H. (1996). *Safflower (*Carthamus tinctorius L.). International Plant Genetic Resources Institute (IPGRI).
  10. Er, C. (1981). Endüstri bitkilerini nadas alanlarına sokabilme olanakları. İçinde Kuru Tarım Bölgelerinde Nadas Alanlarından Yararlanma Sempozyumu(ss. 55-58). TÜBİTAK-TOAG.
  11. Esendal, E., Arslan, B., & Paşa, C. (2008). Effect of winter and spring sowing on yield and plant traits of safflower (Carthamus tinctorius L.). İçinde International Conference Proceedings on Oil Crops(ss. 68).
  12. Gilbert, J. (2008). International safflower production – An overview. İçinde 7th International Safflower Conference(ss. 78).
  13. Iravani, S. (2011). Green synthesis of metal nanoparticles using plants. Green Chemistry, 13(10), 2638–2650. https://doi.org/10.1039/C1GC15386B
  14. Kaviya, S., Santhanalakshmi, J., & Viswanathan, B. (2011). Biosynthesis of silver nanoparticles using Citrus sinensis peel extract and its antibacterial activity. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 79(3–4), 594–598. https://doi.org/10.1016/j.saa.2011.03.040
  15. Kaya, M. D., İpek, A., Uranbey, S., & Kolsarıcı, Ö. (2004). Aspir (Carthamus tinctorius L.)’e uygulanan ethephon’un verim ve verim öğelerine etkileri. Ankara Üniversitesi Ziraat Fakültesi Tarım Bilimleri Dergisi, 10(2), 182–186.
  16. Khalil, M. M. H., Ismail, E. H., El-Baghdady, K. Z., & Mohamed, D. (2014). Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arabian Journal of Chemistry, 7(6), 1131–1139. https://doi.org/10.1016/j.arabjc.2010.11.011
  17. Kim, K. J., Sung, W. S., Moon, S. K., Choi, J. S., Kim, J. G., & Lee, D. G. (2007). Antifungal effect of silver nanoparticles on dermatophytes. Journal of Microbiology and Biotechnology, 18(8), 1482–1484.
  18. Konar, V., Aşkın, Y., & Türkoğlu, İ. (2010). Yabani aspir (Carthamus persicus Willd.) bitkisinin yağ asidi bileşiminin incelenmesi. Fırat Üniversitesi Fen Bilimleri Dergisi, 22(1), 29–36.
  19. Kourkoutas, Y., & Chorianopoulos, N. (Eds.). (2018). Special issue: Novel natural products for drug discovery. BioMed Research International, 2018, Article ID 9875123. https://doi.org/10.1155/2018/9875123
  20. Kumari, S. C., Dhand, V., & Padma, P. N. (2021). Green synthesis of metallic nanoparticles: A review. Nanomaterials, 11(2), 259. https://doi.org/10.3390/nano11020259
  21. López, S., Bermúdez, B., Montserrat-de la Paz, S., Cardelo, M. P., Osorio, P., & Muriana, F. J. (2014). Health effects of oleic acid and long chain omega-3 fatty acids (EPA and DHA) enriched oils. Nutrients, 6(11), 4769–4782. https://doi.org/10.3390/nu6114769
  22. Milshteyn, A., Colosimo, D. A., & Brady, S. F. (2018). Accessing bioactive natural products from the human microbiome. Cell Host & Microbe, 23(6), 725–736. https://doi.org/10.1016/j.chom.2018.05.013
  23. Mittal, A. K., Chisti, Y., & Banerjee, U. C. (2013). Synthesis of metallic nanoparticles using plant extracts. Biotechnology Advances, 31(2), 346–356. https://doi.org/10.1016/j.biotechadv.2013.01.003
  24. Nikaido, H. (2003). Molecular basis of bacterial outer membrane permeability revisited. Microbiology and Molecular Biology Reviews, 67(4), 593–656. https://doi.org/10.1128/MMBR.67.4.593-656.2003
  25. Oğuz, F., & Oğuz, M. N. (2006). Aspir ve hayvan beslemede kullanımı. Yem Magazin, 14(45), 29–33.
  26. Owaid, M. N. (2019). Green synthesis of silver nanoparticles by Pleurotus (oyster mushroom) and their bioactivity: Review. Environmental Nanotechnology, Monitoring & Management, 12, 100256. https://doi.org/10.1016/j.enmm.2019.10025
  27. Rajeshkumar, S.,& Bharath, L. V. (2017). Mechanism of plant-mediated synthesis of silver nanoparticles—A review on biomolecules involved, characterisation and antibacterial activity. Chemico-Biological Interactions, 273, 219–227. https://doi.org/10.1016/j.cbi.2017.06.019
  28. Sengani, M., Grumezescu, A. M., & Rajeswari, V. D. (2017). Recent trends and methodologies in gold nanoparticle synthesis—A prospective review on drug delivery. OpenNano, 2, 37–46. https://doi.org/10.1016/j.onano.2017.07.001
  29. Shamaila, S., Zafar, N., Riaz, S., Sharif, R., Nazir, J., & Naseem, S. (2016). Gold nanoparticles: An efficient antimicrobial agent against enteric bacterial human pathogen. Nanomaterials, 6(4), 71. https://doi.org/10.3390/nano6040071
  30. Shams, G., Ranjbar, M., & Amiri, A. (2013). Effect of silver nanoparticles on concentration of silver heavy element and growth indexes in cucumber (Cucumis sativus L.). Journal of Nanoparticle Research, 15(1), 1630. https://doi.org/10.1007/s11051-013-1630-1
  31. Sharmila, G., Fathima, M. F., Haries, S., Geetha, S., Kumar, N. M., & Muthukumaran, C. (2017). Green synthesis, characterization and antibacterial efficacy of palladium nanoparticles synthesized using Filicium decipiens leaf extract. Journal of Molecular Structure, 1138, 35–40. https://doi.org/10.1016/j.molstruc.2017.02.068
  32. Singh, P., Kim, Y. J., Zhang, D., & Yang, D. C. (2018). Biological synthesis of nanoparticles from plants and microorganisms. Trends in Biotechnology, 34(7), 588–599. https://doi.org/10.1016/j.tibtech.2016.12.006
  33. Taşlıgil, N.,& Şahin, G. (2016). Stratejik önemi artan bir endüstri bitkisi: Aspir (Carthamus tinctorius L.) ve Türkiye’deki coğrafi dağılımı. Türk Coğrafya Dergisi, 67, 91–100.
  34. Vasantharaj, S., Sathiyavimal, S., Senthilkumar, P., & Maruthupandy, M. (2019). Biosynthesis of iron oxide nanoparticles using leaf extract of Ruellia tuberosa: Antimicrobial properties and their applications in photocatalytic degradation. Journal of Photochemistry and Photobiology B: Biology, 192, 74–82. https://doi.org/10.1016/j.jphotobiol.2019.01.017
  35. Weiss, E. A. (2000). Oilseed crops. Blackwell Science Ltd.
  36. Yılmaz, C., & Gökmen, V. (2020). Phenolic compounds in food: Chemistry and antioxidant properties. Current Opinion in Food Science, 32, 126–132. https://doi.org/10.1016/j.cofs.2020.03.009