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Journals / Biyolojik Çeşitlilik ve Koruma / 2026 / Volume: 19 Issue: 2

Molecular phylogenetic analysis of Odiellus lendlii (Soerensen, 1894) from Soğuksu National Park, based on Mitochondrial Cytochrome c oxidase subunit I, 16S rRNA, 18S rRNA, and ITS1/ ITS2 gene markers

Soğuksu Ulusal Park’ta yayılış gösteren Odiellus lendlii (Soerensen, 1894)’nin mitokondriyal sitokrom c oksidaz altünite I, 16S rRNA, 18S rRNA, ITS1/ ITS2 gen markırları temelli moleküler filogenetik analizi

Aslı Özkarakaşoğlu Olacam(KOCAELI UNIVERSITY, INSTITUTE OF SCIENCE)0000-0003-1919-5728
Fikriye Polat(Kocaeli Üniversitesi)0000-0002-5414-2501
İlkay Çorak Öcal(CANKIRI KARATEKIN UNIVERSITY)0000-0003-1479-2697
Pages
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Publication Date
2026-05-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: The molecular data obtained contribute to the genetic characterization and barcoding reference for Odiellus lendlii in this region, supporting future taxonomic and ecological studies.

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: The study aims to characterize molecularly the species Odiellus lendlii of the family Phalangiidae, which occurs in Ankara Soguksu National Park, using DNA barcoding-based methods.
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Key Findings & Data:Method: Samples of Odiellus lendlii collected between June 2022 and September 2023 were used for DNA isolation.
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Conclusion & Impact:Conclusion: The molecular data obtained contribute to the genetic characterization and barcoding reference for Odiellus lendlii in this region, supporting future taxonomic and ecological studies.
🔬Design:Modeling & Simulation
📍Scope:2022 Verileri
⚙️Variables:Odiellus lendlii · COI · 18S rRNA

Abstract

Purpose: The study aims to characterize molecularly the species Odiellus lendlii of the family Phalangiidae, which occurs in Ankara Soguksu National Park, using DNA barcoding-based methods. Method: Samples of Odiellus lendlii collected between June 2022 and September 2023 were used for DNA isolation. PCR and DNA sequencing were performed targeting mitochondrial COI, 18S rRNA, 16S rRNA, ITS1, and ITS2 gene regions using specific primers. FASTA sequences obtained with Chromas were compared to sequences of other Phalangiidae or Odiellus species in the NCBI BLAST database. For phylogenetic analysis, sequences were uploaded to MEGA (v.7.0); the Maximum Likelihood method with Tamura-Nei/Jukes-Cantor model and bootstrap analysis (1000 replicates) was used to construct phylogenetic trees. Findings: This study constitutes the first molecular investigation on Odiellus lendlii distributed in Ankara Soguksu National Park and provides a GenBank submission. Conclusion: The molecular data obtained contribute to the genetic characterization and barcoding reference for Odiellus lendlii in this region, supporting future taxonomic and ecological studies.

Özet

Amaç: Ankara Soğuksu Milli Parkı’nda yayılış gösteren Phalangiidae familyasına ait Odiellus lendlii türünün moleküler karakterizasyonunu DNA barkodlama temelli yöntemlerle araştırmak. Metod: Haziran 2022 ile Eylül 2023 tarihleri arasında toplanan Odiellus lendlii örnekleriyle DNA izolasyonu gerçekleştirilmiştir. Ardından, mitokondriyal COI, 18S rRNA, 16S rRNA, ITS1 ve ITS2 genlerinin primerleri kullanılarak PCR ve DNA dizi analizi yapılmıştır. Chromas ile elde edilen FASTA dizileri, NCBI BLAST veri tabanındaki diğer Phalangiidae veya Odiellus türlerinin dizileriyle karşılaştırılmıştır. Filogenetik analiz için diziler MEGA (v.7.0) programına yüklenmiş ve Maksimum Likelihood yöntemi, Tamura-Nei/Jukes Cantor Modeli, Bootstrap 1000 ile filogenetik ağaçlar oluşturulmuştur. Bulgular: Çalışma, Ankara Soğuksu Milli Parkı’nda yayılış gösteren Odiellus lendlii üzerine yapılan ilk moleküler çalışmayı oluşturmaktadır ve GenBank’a bir kayıt sağlamaktadır. Sonuç: Elde edilen moleküler veriler, bu tür için bu bölgede genetik karakterizasyon ve barkod tabanlı referans oluşturulmasına katkıda bulunmakta olup gelecekteki taksonomi ve ekoloji çalışmalarını desteklemektedir.

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Cite This Article

Olacam, A. Ö., Polat, F., Öcal, İ. Ç. (2026). Molecular phylogenetic analysis of Odiellus lendlii (Soerensen, 1894) from Soğuksu National Park, based on Mitochondrial Cytochrome c oxidase subunit I, 16S rRNA, 18S rRNA, and ITS1/ ITS2 gene markers. Biyolojik Çeşitlilik ve Koruma, Volume: 19 Issue: 2. https://doi.org/10.46309/biodicon.2026.1829086

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