SavedAdd your journal

Journals / ADO Klinik Bilimler Dergisi / 2024 / Cilt: 13 Sayı: 3

Effect of Different MMP Inhibitors on the Bond Strength and Durability of an Etch-and-rinse and a Self-etch Adhesive

Farklı MMP İnhibitörlerinin Etch-and-rinse ve Self-etch Adezivin Bağlanma Kuvveti ve Dayanıklılığı Üzerine Etkisi

Ahmet Hazar(ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ)0000-0002-3931-5179
Mine Betül Üçtaşlı(GAZİ ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ)0000-0002-5670-6921
Pages
453–460
Publication Date
2024-09-24
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: The use of 2% HPN and 1% BAC as a separate step before the adhesive application can be recommended to preserve the longevity of resin-dentin bond strength on etch-and-rinse and self-etch adhesives.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: To compare the effect of different matrix metalloproteinase (MMP) inhibitors on the microtensile bond strength (μTBS) of an etch-and-rinse adhesive and a self-etch adhesive at baseline and after aging.
📊
Key Findings & Data:Materials and Method: Flat dentin surfaces were prepared from 96 extracted, sound human molars.
💡
Conclusion & Impact:Conclusion: The use of 2% HPN and 1% BAC as a separate step before the adhesive application can be recommended to preserve the longevity of resin-dentin bond strength on etch-and-rinse and self-etch adhesives.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Adezivler · Benzalkonyum klorür · klorheksidin diglukonat

Abstract

Aim: To compare the effect of different matrix metalloproteinase (MMP) inhibitors on the microtensile bond strength (μTBS) of an etch-and-rinse adhesive and a self-etch adhesive at baseline and after aging. Materials and Method: Flat dentin surfaces were prepared from 96 extracted, sound human molars. Teeth were randomly divided into two main groups according to the adhesives (Adper Single Bond 2, and Clearfil 3S Plus) used (n=48). Each group was divided into four subgroups according to the dentin surface treatments; untreated (control), pretreated with 2% chlorhexidine digluconate (CHX) before adhesive application, pretreated with 1% benzalkonium chloride (BAC) before adhesive application, and pretreated with 2% hesperidin (HPN) before adhesive application (n=12). Composite resin was then placed on the prepared surfaces and polymerized. The specimens were cut to obtain dentin beams and randomly divided into two groups for microtensile analysis at baseline and after 6 months of aging. Failure types were observed test after microtensile tests and data were analyzed by Two-way ANOVA, Tukey HSD, and Chi-Square tests (p <0.05). Results: Compared with control, all MMP inhibitors significantly preserved and improved the bond strength of both adhesives at baseline and after 6 months of aging (p < 0.05). When considering the comparison of MMP inhibitors, bond strengths of both adhesives in the HPN and BAC groups were significantly higher than CHX group after aging (p < 0.05). The failure types were mostly adhesive and mixed. Conclusion: The use of 2% HPN and 1% BAC as a separate step before the adhesive application can be recommended to preserve the longevity of resin-dentin bond strength on etch-and-rinse and self-etch adhesives.

Özet

Amaç: Farklı matriks metalloproteinaz (MMP) inhibitörlerinin, başlangıç ve yaşlandırma sonrasında bir pürüzlendirmeli ve yıkamalı adeziv ile bir kendinden pürüzlendirmeli adezivin mikrogerilim bağlanma kuvveti (μTBS) üzerindeki etkisini karşılaştırmak. Gereç ve yöntem: 96 adet çekilmiş sağlam insan azı dişinden düz dentin yüzeyleri hazırlandı. Dişler kullanılan adezivlere (Adper Single Bond 2 ve Clearfil 3S Plus) göre rastgele iki ana gruba ayrıldı (n=48). Her grup dentin yüzey işlemlerine göre dört alt gruba ayrıldı; işlem uygulanmayan (kontrol), adeziv uygulanmadan önce %2 klorheksidin diglukonat uygulaması (CHX), adeziv uygulanmadan önce %1 benzalkonyum klorür (BAC) uygulaması ve adeziv uygulanmadan önce %2 hesperidin (HPN) uygulaması (n=12). Daha sona hazırlanan yüzeylere kompozit rezin yerleştirilip polymerize edildi. Örnekler, dentin çubukları elde etmek için kesildi, başlangıç ve 6 aylık yaşlandırmadan sonra mikrotensil analizi için rastgele iki gruba ayrıldı. Mikrogerilim testleri sonrasında kırık tipleri gözlemlendi ve veriler Two-way ANOVA, Tukey HSD ve Ki-Kare testleri ile analiz edildi (p <0.05). Bulgular: Kontrol ile karşılaştırıldığında, tüm MMP inhibitörleri başlangıçta ve 6 aylık yaşlandırmadan sonra her iki adezivin bağlanma kuvvetini önemli ölçüde korumuş ve iyileştirmiştir (p < 0.05).MMP inhibitörlerinin karşılaştırılması göz önüne alındığında, HPN ve BAC gruplarındaki her iki adezivin bağlanma kuvvetleri, yaşlandırma sonrasında CHX grubundan önemli ölçüde daha yüksekti (p < 0.05). Başarısızlık türleri çoğunlukla adeziv ve karışık olarak görüldü. Sonuç: Pürüzlendirmeli ve yıkamalı, kendinden pürüzlendirmeli adezivlerde rezin-dentin bağlanma kuvvetinin uzun ömürlülüğünü korumak için adeziv uygulamasından önce ayrı bir adım olarak %2 HPN ve %1 BAC kullanımı önerilebilir.

🔖

Cite This Article

Hazar, A., Üçtaşlı, M. B. (2024). Effect of Different MMP Inhibitors on the Bond Strength and Durability of an Etch-and-rinse and a Self-etch Adhesive. ADO Klinik Bilimler Dergisi, Cilt: 13 Sayı: 3, 453–460. https://doi.org/10.54617/adoklinikbilimler.1485108

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (29)

  1. Kusumasari C, Hatayama T, Shimada Y. Replacement of two failed indirect restorations with direct bonded restorations using minimally invasive strategy. J Dent Indones 2021;28:198-204.
  2. de Moraes IQS, do Nascimento TG, da Silva AT, de Lira LMSS, Parolia A, Porto ICCM. Inhibition of matrix metalloproteinases: a troubleshooting for dentin adhesion. Restor Dent Endod 2020;45:e31.
  3. Wang L, Agulhari MAS, Froio NL, Campos MMB, Mosquim V, Brianezzi LF de F, D’Alpino NRS, Honório HM. 10-MDP-Based Dentin Bonding Systems: Why Is Chlorhexidine Not Worth to Be Used Adjunctively? J Adhes 2023;37:3569–81.
  4. Kiuru O, Sinervo J, Vähänikkilä H, Anttonen V, Tjäderhane L. MMP inhibitors and dentin bonding: systematic review and meta-Analysis. Int J Dent 2021;2021:9949699.
  5. Montagner AF, Sarkis-Onofre R, Pereira-Cenci T, Cenci MS. MMP inhibitors on dentin stability: a systematic review and meta-analysis. J Dent Res 2014;93:733-43.
  6. Tezvergil-Mutluay A, Mutluay MM, Gu LS, Zhang K, Agee KA, Carvalho RM, Manso A, et al. The anti-MMP activity of benzalkonium chloride. J Dent 2011;39:57-64.
  7. Beck F, Ilie N. Antioxidants and collagen-crosslinking: benefit on bond strength and clinical applicability. Materials (Basel) 2020;13:5483.
  8. Dávila-Sánchez A, Gutierrez MF, Bermudezd JP, María Luján Méndez-Bauerd ML, Hilgembergd B, Sauro S, et al. Influence of flavonoids on long-term bondingstability on caries-affected dentin. Dent Mater 2020;36:1151-60.
  9. Islam S, Hiraishi N, Nassar M, Yiu C, Otsuki M, Tagami J. Effect of hesperidin incorporation into a self-etching primer on durability of dentin bond. Dent Mater 2014;30:1205-12.
  10. Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, Ito S. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004;83:216-21.
  11. Ting S, Chowdhury AA, Pan F, Fu J, Sun J, Kakuda S,et al. Effect of remaining dentin thickness on microtensile bond strength of current adhesive systems. Dent Mater J 2015;34:181-8.
  12. Cadenaro M, Pashley DH, Marchesi G, Carrilho M, Antoniolli F, Mazzoni A, et al. Influence of chlorhexidine on the degree of conversion and E-modulus of experimental adhesive blends. Dent Mater 2009;25:1269-74.
  13. Collares FM, Rodrigues SB, Leitune VC, Celeste RK, Borba de Araújo F, Samuel SM. Chlorhexidine application in adhesive procedures: a meta-regression analysis. J Adhes Dent 2013;15:11-8.
  14. Muñoz MA, Luque-Martinez I, Malaquias P, Hass V, Reis A, Campanha NH, et al. In vitro longevity of bonding properties of universal adhesives to dentin. Oper Dent 2015;40:282-92.
  15. Kim J, Uchiyama T, Carrilho M, Agee KA, Mazzoni A, Breschi L, et al. Chlorhexidine binding to mineralized versus demineralized dentin powder. Dent Mater 2010;26:771-8.
  16. Sabatini C, Kim JH, Ortiz PA. In vitro evaluation of benzalkonium chloride in the preservation of adhesive interfaces. Oper Dent 2014;39:283-90.
  17. Hazar A, Akgül S, Hazar E. Effect of chlorhexidine and benzalkonium chloride on the long-term push-out bond strength of fiber posts. Niger J Clin Pract 2023;26:1242-1248.
  18. Comba A, Maravic T, Valente L, Girlando M, Cunha S R, Checchi V, et al. Effect of benzalkonium chloride on dentin bond strength and endogenous enzymatic activity. J Dent 2019;85:25-32.
  19. Islam S, Hiraishi N, Nassar M, Yiu C, Otsuki M, Tagami J. Effect of natural cross-linkers incorporation in a self-etching primer on dentine bond strength. J Dent 2012;40:1052-9.
  20. Zheng P, Zaruba M, Attin T, Wiegand A. Effect of different matrix metalloproteinase inhibitors on microtensile bond strength of an etch-and-rinse and a self-etching adhesive to dentin. Oper Dent 2015;40:80-6.
  21. Mazzoni A, Scaffa P, Carrilho M, Tja¨derhane L, Di Lenarda R, Polimeni A, et al. Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9. J Dent Res. 2013;92(1):82-6.
  22. Frassetto A, Breschi L, Turco G, Marchesi G, Di Lenarda R, Tay FR, et al. Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability—A literature review. Dent Mater 2016;32:41-53.
  23. Zheng P, Chen H. Evaluate the effect of different MMPS inhibitors on adhesive physical properties of dental adhesives, bond strength and MMP substrate activity. Sci Rep 2017;7:4975.
  24. Sabatini C, Pashley DH. Aging of adhesive interfaces treated with benzalkonium chloride and benzalkonium methacrylate. Eur J Oral Sci 2015;123:102-7.
  25. Xu J, Li M, Wang W, Wu Z, Wang C, Xiaoting Jin X, et al. A novel prime-&-rinse mode using MDP and MMPs inhibitors improves the dentin bond durability of self-etch adhesive. J Mech Behav Biomed Mater 2020;104:103698.
  26. El Gezawi M, Haridy R, Abo Elazm E, Al-Harbi F, Zouch M, Kaisarly D. Microtensile bond strength, 4-point bending and nanoleakage of resin-dentin interfaces: Effects of two matrix metalloproteinase inhibitors. J Mech Behav Biomed Mater 2018;78:206-13. Tekçe N, Tuncer S, Demirci M, Balci S. Do matrix metalloproteinase inhibitors improve the bond durability of universal dental adhesives? Scanning. 2016;38:535-44.
  27. Hiraishi N, Sono R, Islam MS, Otsuki M, Tagami J, Takatsuka T. Effect of hesperidin in vitro on root dentine collagen and demineralization. J Dent 2011;39:391-6.
  28. Scherrer SS, Cesar PF, Swain MV. Direct comparison of the bond strength results of the different test methods: a critical literature review. Dent Mater 2010;26:78-93.
  29. Campos EA, Correr GM, Leonardi DP, Pizzatto E, Morais EC. Influence of chlorhexidine concentration on microtensile bond strength of contemporary adhesive systems. Braz Oral Res 2009;23:340-5.