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Journals / Kocaeli Journal of Science and Engineering / 2026 / Cilt: 2026 Sayı: 17

Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.)

Siyah Dut (Morus nigra L.) Polifenol Oksidaz Enziminin Ekstraksiyonu

Simge Çolak(Kocaeli Üniversitesi)0009-0009-5314-8993
Semih Işık(Kocaeli Üniversitesi)0000-0002-2035-6480
Elif Kale Bakır(Kocaeli Üniversitesi)0000-0002-4266-9517
Yonca Yüzügüllü Karakuş(KOCAELİ ÜNİVERSİTESİ)0000-0003-0286-8711
Pages
153–160
Publication Date
2026-10-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Overall, this study provides a cost-effective, efficient protocol for PPO recovery, offering significant industrial relevance for food biotechnology.

📌 3 Key Takeaways

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Objective & Hypothesis:This study focuses on developing a high-efficiency, cost-effective three-phase partitioning (TPP) purification strategy for polyphenol oxidase (PPO) from black mulberry (Morus nigra) fruit, known for its rich antioxidant profile.
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Key Findings & Data:Reaction conditions were optimized, and maximum PPO activity was achieved in a system containing 100 mM sodium phosphate buffer (pH 7.0), 10 mM ascorbic acid, 0.5% (w/v) PEG 6000, and 50 mM K₂HPO₄.
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Conclusion & Impact:Overall, this study provides a cost-effective, efficient protocol for PPO recovery, offering significant industrial relevance for food biotechnology.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Morus nigra · Polifenol oksidaz · Üçlü-faz ayırma

Abstract

This study focuses on developing a high-efficiency, cost-effective three-phase partitioning (TPP) purification strategy for polyphenol oxidase (PPO) from black mulberry (Morus nigra) fruit, known for its rich antioxidant profile. Reaction conditions were optimized, and maximum PPO activity was achieved in a system containing 100 mM sodium phosphate buffer (pH 7.0), 10 mM ascorbic acid, 0.5% (w/v) PEG 6000, and 50 mM K₂HPO₄. Under optimized TPP conditions, PPO was purified with an exceptionally high activity recovery of 177% and a 2.7-fold increase in purity, demonstrating the efficiency of this approach compared with conventional purification strategies. The methodological simplicity, scalability, and environmentally friendly nature of the process highlight its potential as a practical alternative for enzyme purification from natural sources. Overall, this study provides a cost-effective, efficient protocol for PPO recovery, offering significant industrial relevance for food biotechnology.

Özet

Bu çalışma, yüksek likopen içeriği ve antioksidan özellikleriyle bilinen kara dut (Morus nigra) meyvesinde polifenol oksidaz (PPO) enziminin varlığını incelemeyi ve etkin bir saflaştırma süreci geliştirmeyi amaçlamaktadır. Çalışmada öncelikle enzim aktivitesi optimize edildi ve en yüksek PPO aktivitesinin 100 mM sodyum fosfat tamponu (pH 7,0), 10 mM askorbik asit, %0,5 (a/a) PEG 6000 ve 50 mM K₂HPO₄ içeren reaksiyon karışımında elde edildiği belirlendi. Saflaştırma sürecinde, güncel ve etkili teknikler olan aseton çöktürmesi ve üç fazlı bölümlendirme (three-phase partitioning, TPP) yöntemleri birlikte kullanıldı. PPO enzimi %177 verim ve 2,7 kat saflık artışı ile saflaştırıldı. Elde edilen sonuçlar, likopen açısından zengin kara dut meyvesinden enzim ekstraksiyonu için geliştirilen bu yöntemin güvenilir, düşük maliyetli ve çevre dostu bir alternatif sunduğunu göstermektedir. Bu çalışmada geliştirilen yüksek verimli saflaştırma stratejisinin, gıda endüstrisi ve biyoteknolojik uygulamalar için önemli bir ekonomik avantaj sağlayacağı düşünülmektedir. Ayrıca bu bulguların, doğal kaynaklardan endüstriyel enzim üretim süreçlerine katkı sağlaması beklenmektedir.

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Cite This Article

Çolak, S., Işık, S., Bakır, E. K., Karakuş, Y. Y. (2026). Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.). Kocaeli Journal of Science and Engineering, Cilt: 2026 Sayı: 17, 153–160. https://doi.org/10.34088/kojose.1875986

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