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Journals / Journal of Clinical and Experimental Investigations / 2010 / Cilt: 1 Sayı: 3

A different insight into blood coagulation in vitro

İnvitro pıhtılaşmaya farklı bir bakış açısı

Osman Evliyaoğlu
Selvi Kelekçi
M. Kemal Başaralı
Birgül Işık
Beri Hocaoğlu Bozarslan
Hanım Karahan
Pages
173–176
Publication Date
2010-09-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Serum aspartate (asp), glutamate (glu), serine (ser), histidine (his) and phenylalanine (phe) levels were significantly higher in serum than plasma (p

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: The known model of blood coagulation involves a series of zymogen activation reaction sequences.
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Key Findings & Data:Methods: Samples of serum and plasma (treated by EDTA or citrate) were obtained from healthy donors and from patients with Phenylketonuria (PKU).
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Conclusion & Impact:Serum aspartate (asp), glutamate (glu), serine (ser), histidine (his) and phenylalanine (phe) levels were significantly higher in serum than plasma (p
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Pıhtılaşma · amino asit · Fenilketonüri

Abstract

Objectives: The known model of blood coagulation involves a series of zymogen activation reaction sequences. At each stage, a zymogen is converted to an active protease by cleavage of one or more peptide bonds in the precursor molecule. The aim of this study was to investigate amino acid profiles during coagulation process in different conditions in vitro. Methods: Samples of serum and plasma (treated by EDTA or citrate) were obtained from healthy donors and from patients with Phenylketonuria (PKU). Amino acid profiles analyzed with reverse phase HPLC column. Results: There were no differences between two plasma amino acid levels which were obtained by EDTA and acid citrate (p>0.05). Serum aspartate (asp), glutamate (glu), serine (ser), histidine (his) and phenylalanine (phe) levels were significantly higher in serum than plasma (p

Özet

Amaç: Koagülasyon bilindiği üzere bir dizi zimojenin aktivasyon serisinden oluşur. Her basamakta öncül molekül bir veya daha fazla peptit bağının kopmasıyla aktif proteaza dönüşür. Bu çalışmanın amacı in vitro farklı durumlarda pıhtılaşma sırasında amino asit profilini araştırmaktır. Yöntem: Sağlıklı yetişkinlerden ve fenilketonürili çocuklardan alınan örnekler serum, ETA\'lı plazma ve sitratlı plazma olarak ayrıldı. Örneklerin aminoasit analizleri ters faz HPLC kolonuyla yapıldı. Bulgular: EDTA\'lı ve sitratlı plazmalar arasında anlamlı bir fark görülmedi (p>0.05). Serum aspartat (asp), glutamate (glu), serin (ser), histidin (his) ve fenilalanin (phe) seviyelerinde plazmaya göre anlamlı bir artış kaydedildi (p

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Cite This Article

Evliyaoğlu, O., Kelekçi, S., Başaralı, M. K., Işık, B., Bozarslan, B. H., Karahan, H. (2010). A different insight into blood coagulation in vitro. Journal of Clinical and Experimental Investigations, Cilt: 1 Sayı: 3, 173–176. https://doi.org/10.5799/ahinjs.01.2010.03.0036

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