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Journals / GORM:Gynecology Obstetrics & Reproductive Medicine / 2009 / Cilt: 15 - Sayı: 2

Gebe Kadınlarda Beyin Omurilik Sıvısı ve Serum S-100B Protein Ölçümünün Serebral Hasarı Saptamadaki Rolü

Logical Basis of Cerebrospinal Fluid and Serum S-100B Protein Measurement in Pregnant Women to Detect any Possible Cerebral Damage

Nilüfer ÇELİK
Önder ÇELİK
Halis CANPOLAT
Osman EVLİYAOĞLU
H. Muammer KARAKAŞ
Pages
81–84
DOI
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⚡ TL;DR(Too Long; Didn't Read)

DISCUSSION: These results prove that the maternal serum and CSF S100B protein levels are independently related to each other in pregnancy.

📌 3 Key Takeaways

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Objective & Hypothesis:OBJECTIVE: The aim of the study was to consider logical basis of serum and cerebrospinal fluid (CSF) S100B measurement in pregnant females to detect any cerebral damage.
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Key Findings & Data:STUDY DESIGN: CSF and serum samples from 14 pregnant patients (age: 20-34 years) were obtained during spinal anesthetic procedure of cesarean section.
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Conclusion & Impact:DISCUSSION: These results prove that the maternal serum and CSF S100B protein levels are independently related to each other in pregnancy.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVE: The aim of the study was to consider logical basis of serum and cerebrospinal fluid (CSF) S100B measurement in pregnant females to detect any cerebral damage. STUDY DESIGN: CSF and serum samples from 14 pregnant patients (age: 20-34 years) were obtained during spinal anesthetic procedure of cesarean section. The serum samples from 9 non-pregnant patients (22-36 years) without an organic brain disease were used as normative data. S-100B levels in serum and CSF samples were measured with electrochemiluminescence immunoassay method. The CSF and blood serum levels of pregnants and blood serum levels of pregnant and nonpregnant females were compared using Kruskal-Wallis and Mann-Whitney U-tests. RESULTS: Serum S100B protein levels of pregnant females were significantly higher [0.66±0.06 ng/ml] than those observed in [0.06±0.00 ng/ml] nonpregnant females (p<.0001). There was a significant difference between the S100B protein levels of CSF and blood serum (p<0.05) in pregnant females. However the correlation between these two levels was insignificant (p=0.473). CSF S100B levels of pregnant subjcets were significantly higher than the serum levels of pregnant and nonpregnant subjects (p<0.0001). DISCUSSION: These results prove that the maternal serum and CSF S100B protein levels are independently related to each other in pregnancy.

Özet

AMAÇ: Bu çalışma serum ve beyin omurilik sıvısı (BOS) S100B protein düzey ölçümlerinin gebelerde serebral hasarı saptamadaki rolünü araştırmak için planlandı. GEREÇ VE YÖNTEM: Yaşları 20-34 arasında değişen 14 gebe hastadan sezaryen operasyonu için yapılan spinal anestezi esnasında serum ve BOS örnekleri alındı. Gebe olmayan ve organik bir beyin hastalığı bulunmayan 9 sağlıklı kadından alınan serum örnekleri ise kontrol grubu olarak belirlendi. Serum ve BOS S100B protein düzeyleri electrochemiluminescence yöntemiyle çalışıldı. Kontrol grubu ve gebelerin serum ve BOS S100B düzeyleri Kruskal-Wallis ve Mann-Whitney Utest kullanılarak karşılaştırıldı. BULGULAR: Gebe kadınların serum S100B protein düzeyleri [0.66 ± 0.06 ng/ml] gebe olmayanlardan [0.06 ± 0.00 ng/ml] anlamlı olarak daha yüksek saptandı (p<.0001). Gebe kadınların BOS S100B protein düzeyleri serum S100B protein düzeylerinden anlamlı olarak yüksek bulundu (p<0.05). Ancak iki dü- zey arasındaki korelasyon anlamlı değildi (p=0.473). Ge be ler - de BOS S100B protein düzeyleri gebe olmayanların serum S100B protein düzeylerinden anlamlı olarak yüksek saptandı (p<0.0001).

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Cite This Article

ÇELİK, N., ÇELİK, Ö., CANPOLAT, H., EVLİYAOĞLU, O., KARAKAŞ, H. M. (2009). Gebe Kadınlarda Beyin Omurilik Sıvısı ve Serum S-100B Protein Ölçümünün Serebral Hasarı Saptamadaki Rolü. GORM:Gynecology Obstetrics & Reproductive Medicine, Cilt: 15 - Sayı: 2, 81–84. https://ajindex.com/articles/227069

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References (14)

  1. Donato R. S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles.Int J Biochem Cell Biol. 2001;33:637-68
  2. Marks A, Allore R. S100 protein and Down syndrome. Bioessays. 1990;12:381-3
  3. Endo T, Tanaka T, Isobe T, Kasai H, Okuyama T, Hidaka H. Calcium-dependent affinity chromatography of S-100 and calmodulin on calmodulin antagonist-coupled Sepharose. J Biol Chem. 1981;256:12485-9
  4. Rickmann M, Wolff JR. S100 protein expression in subpopulations of neurons of rat brain. Neuroscience. 1995;67:977-91
  5. Isobe T, Takahashi K, Okuyama T. S100a0 (alpha alpha) protein is present in neurons of the central and peripheral nervous system. J Neurochem. 1984;43:1494-6
  6. Haimoto H, Hosoda S, Kato K. Differential distribution of immunoreactive S100-alpha and S100-beta proteins in normal nonnervous human tissues. Lab Invest. 1987;57: 489-98
  7. Marinoni E, Di Iorio R, Gazzolo D, Lucchini C, Michetti F, Corvino V, Cosmi EV. Ontogenetic localization and dis- tribution of S-100beta protein in human placental tissues. Obstet Gynecol. 2002;99:1093-9
  8. Michetti F, Dell'Anna E, Tiberio G, Cocchia D. Immunochemical and immunocytochemical study of S- 100 protein in rat adipocytes. Brain Res. 1983;262:352-6
  9. Persson L, Hardemark HG, Gustafsson J, Rundstrom G, Mendel-Hartvig I, Esscher T, Pahlman S. S-100 protein and neuron-specific enolase in cerebrospinal fluid and serum: markers of cell damage in human central nervous system. Stroke. 1987;18:911-8
  10. Ingebrigtsen T, Waterloo K, Jacobsen EA, Langbakk B, Romner B. Traumatic brain damage in minor head injury: relation of serum S-100 protein measurements to magnetic resonance imaging and neurobehavioral outcome. Neurosurgery. 1999;45:468-75; discussion 475-6
  11. Wunderlich MT, Ebert AD, Kratz T, Goertler M, Jost S, Herrmann M. Early neurobehavioral outcome after stroke is related to release of neurobiochemical markers of brain damage. Stroke. 1999;30:1190-5
  12. Lara DR, Gama CS, Belmonte-de-Abreu P, Portela LV, Goncalves CA, Fonseca M, Hauck S, Souza DO. Increased serum S100B protein in schizophrenia: a study in medication-free patients. J Psychiatr Res. 2001;35:11-4
  13. Michetti F, Massaro A, Russo G, Rigon G. The S-100 antigen in cerebrospinal fluid as a possible index of cell injury in the nervous system. J Neurol Sci. 1980;44:259-63
  14. Sindic CJ, Chalon MP, Cambiaso CL, Laterre EC, Masson PL. Assessment of damage to the central nervo