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Journals / International Advanced Researches and Engineering Journal / 2026 / Volume: 10 Issue: 1

Hepatic bioavailability and cellular response to microfluidic synthesized FITC@p(GMA-EGDMA) nanoparticles

Atakan Tevlek(ATILIM ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0003-0601-8642
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Publication Date
2026-04-20
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Collectively, the results support the use of fluorescein-labeled poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) nanoparticles as biocompatible and traceable platforms for short- to mid-term in vitro imaging and drug delivery applications, with suggested operational concentrations below 50 µg/mL to ensure minimal functional interference.

📌 3 Key Takeaways

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Objective & Hypothesis:Fluorescent polymeric nanoparticles have emerged as versatile tools in biomedical imaging due to their tunable optical properties and potential for biological integration.
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Key Findings & Data:In this study, fluorescein isothiocyanate-labeled poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) nanoparticles synthesized via microfluidic methods were evaluated for their biocompatibility, cytotoxicity, cellular uptake, and functional effects in HepG2 human hepatocellular carcinoma cells, a widely used model for assessing nanoparticle biocompatibility and hepatic metabolism.
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Conclusion & Impact:Collectively, the results support the use of fluorescein-labeled poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) nanoparticles as biocompatible and traceable platforms for short- to mid-term in vitro imaging and drug delivery applications, with suggested operational concentrations below 50 µg/mL to ensure minimal functional interference.
🔬Design:Modeling & Simulation
📍Scope:2009 Verileri
⚙️Variables:Biocompatibility · Cytotoxicity · Fluorescent Nanoparticles

Abstract

Fluorescent polymeric nanoparticles have emerged as versatile tools in biomedical imaging due to their tunable optical properties and potential for biological integration. In this study, fluorescein isothiocyanate-labeled poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) nanoparticles synthesized via microfluidic methods were evaluated for their biocompatibility, cytotoxicity, cellular uptake, and functional effects in HepG2 human hepatocellular carcinoma cells, a widely used model for assessing nanoparticle biocompatibility and hepatic metabolism. A concentration range of 0–100 µg/mL was applied over 24, 48, and 72 hours. In vitro cytotoxicity, assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, revealed significant dose- and time-dependent effects on HepG2 cell viability. Notably, at 24 hours all tested concentrations, including 100 µg/mL, maintained cell survival above the 70% non-cytotoxic threshold defined by ISO 10993-5:2009, whereas prolonged exposure at 100 µg/mL resulted in values below this limit. Fluorescence microscopy confirmed dose-dependent cellular association of the nanoparticles without compromising cell morphology. Acridine Orange/Propidium Iodide staining corroborated the cytotoxicity profile by demonstrating dose-dependent increases in membrane damage, consistent with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results. Furthermore, a scratch wound assay demonstrated impaired migration capacity at ≥50 µg/mL after 24 hours, indicating a functional cellular response even in the absence of overt cytotoxicity. Stability assessments showed preserved colloidal and structural integrity over 72 hours, although fluorescence intensity declined over time. Collectively, the results support the use of fluorescein-labeled poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) nanoparticles as biocompatible and traceable platforms for short- to mid-term in vitro imaging and drug delivery applications, with suggested operational concentrations below 50 µg/mL to ensure minimal functional interference.

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Cite This Article

Tevlek, A. (2026). Hepatic bioavailability and cellular response to microfluidic synthesized FITC@p(GMA-EGDMA) nanoparticles. International Advanced Researches and Engineering Journal, Volume: 10 Issue: 1. https://doi.org/10.35860/iarej.1804524

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References (44)

  1. Al-Thani, A.N., A.G. Jan, M. Abbas, M. Geetha, and K.K. Sadasivuni, Nanoparticles in cancer theragnostic and drug delivery: A comprehensive review. Life Sciences, 2024. 352: p. 122899.
  2. Du, Y., J. Xu, T. Han, Z. Jiang, Y. Zhang, and J. Li, Albumin-seeking dyes with adjustable assemblies in situ enable programmable imaging windows and targeting tumor imaging. Theranostics, 2024. 14: p. 2675–2686.
  3. Sheikholeslami, S.A., J. Esmaeili, S.Z. Jalise, and A. Barati, A response surface methodology study on the development of pH-sensitive wound dressings using Rhodamine B-loaded chitosan nanoparticles and sodium alginate-based films. Heliyon, 2024. 10: p. e40670.
  4. Alrubaye, M.A., Z. Lafi, M.N. Abu Hajleh, T.M.M. Abuamara, and A. Mehdawi, Co-encapsulation of coumarin and Q10 into solid lipid nanoparticles for wound healing. Journal of Drug Delivery Science and Technology, 2025. 105: p. 106645.
  5. Shen, Z., F. Zhang, J. Yang, K. Zhang, F. Liang, and H. Mu, Novel mitochondria-targeted NIR cyanine Cy750M-C1 nanoparticles for chemotherapy against triple-negative breast cancer. ACS Biomaterials Science & Engineering, 2025. 11: p. 3738–3751.
  6. Chen, Y., H. Liu, and D. Zhang, Small molecular fluorescent probes featuring protein-assisted functional amplification for improved biosensing and cancer therapeutics. Chemical Communications, 2025. 61: p. 7908–7928.
  7. Ilavenil, K.K., V. Senthilkumar, and A. Kasthuri, Green synthesis of metal nanoparticles from three medicinal plants: a review of environmental and health applications. Discover Catalysis, 2025. 2: p. 00007.
  8. Wang, X., Y. Feng, J. Liu, K. Cheng, Y. Liu, and W. Yang, et al., Fluorescein isothiocyanate-doped conjugated polymer nanoparticles for two-photon ratiometric fluorescent imaging of intracellular pH fluctuations. Spectrochimica Acta A: Molecular and Biomolecular Spectroscopy, 2022. 267: p. 120477.
  9. Zhong, H., B. Zhao, and J. Deng, Synthesis and application of fluorescent polymer micro- and nanoparticles. Small, 2023. 19: p. 300961.
  10. Milosevic, A.M., L. Haeni, L. Ackermann Hirschi, S. Vanni, P. Campomanes-Ramos, B. Rothen-Rutishauser, et al., The choice of nanoparticle surface-coupled fluorescent dyes impacts cellular interaction. ChemNanoMat, 2022. 8: p. 100443.
  11. Xiao, J., Q. Lu, H. Cong, Y. Shen, and B. Yu, Microporous poly(glycidyl methacrylate-co-ethylene glycol dimethyl acrylate) microspheres: synthesis, functionalization and applications. Polymer Chemistry, 2021. 12: p. 6050–6070.
  12. Kılınçlı, B., A.D. Çınar, B. Çetin, and G. Kibar, Microfluidic vs. batch synthesis of fluorescent poly(GMA-co-EGDMA) micro/nanoparticles for biomedical applications. Emergent Materials, 2025. 8: p. 1101–1110.
  13. Chen, H., J. Guo, Y. Wang, W. Dong, Y. Zhao, and L. Sun, Bio-inspired imprinting materials for biomedical applications. Advanced Science, 2022. 9: p. 2022038.
  14. Kibar, G., U. Çalışkan, E.Y. Erdem, and B. Çetin, One-pot synthesis of organic–inorganic hybrid polyhedral oligomeric silsesquioxane microparticles in a double-zone temperature controlled microfluidic reactor. Journal of Polymer Science A: Polymer Chemistry, 2019. 57: p. 1396–1403.
  15. Agha, A., W. Waheed, I. Stiharu, V. Nerguizian, G. Destgeer, and E. Abu-Nada, A review on microfluidic-assisted nanoparticle synthesis and their applications using multiscale simulation methods. Discover Nano, 2023. 18: p. 3792.
  16. Stefanović, I.S., B.M. Ekmeščić, D.D. Maksin, A.B. Nastasović, Z.P. Miladinović, and Z.M. Vuković, Structure, thermal, and morphological properties of novel macroporous amino-functionalized glycidyl methacrylate based copolymers. Industrial & Engineering Chemistry Research, 2015. 54: p. 6902–6911.
  17. Kibar, G., S. Dutta, K. Rege, and O.B. Usta, Evaluation of drug carrier hepatotoxicity using primary cell culture models. Nanomedicine, 2023. 48: p. 102651.
  18. Tevlek, A., G. Kibar, and B. Cetin, Assessment of anticancer effects of Aloe vera on 3D liver tumor spheroids in a microfluidic platform. Biotechnology and Bioengineering, 2025.
  19. Geraghty, R.J., A. Capes-Davis, J.M. Davis, J. Downward, R.I. Freshney, and I. Knezevic, Guidelines for the use of cell lines in biomedical research. British Journal of Cancer, 2014. 111: p. 1021–1046.
  20. Xu, J.J., D. Diaz, and P.J. O’Brien, Applications of cytotoxicity assays and pre-lethal mechanistic assays for assessment of human hepatotoxicity potential. Chemical Biology Interactions, 2004. 150: p. 115–128.
  21. Gerets, H.H.J., K. Tilmant, B. Gerin, H. Chanteux, B.O. Depelchin, and S. Dhalluin, Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers. Cell Biology and Toxicology, 2012. 28: p. 69–87.
  22. Ozdemir, N., G. Tan, A. Tevlek, G. Arslan, G. Zengin, and I. Sargin, Dead cell discrimination with red emissive carbon quantum dots from Echinophora tenuifolia. Journal of Fluorescence, 2025. p. 1-18.
  23. Bielecka-Kowalska, A., P. Czarny, P. Wigner, E. Synowiec, B. Kowalski, and M. Szwed, Ethylene glycol dimethacrylate and diethylene glycol dimethacrylate exhibit cytotoxic and genotoxic effects on human gingival fibroblasts. Toxicology in Vitro, 2018. 47: p. 8–17.
  24. Loutfy, S.A., H.M.A. El-Din, M.H. Elberry, N.G. Allam, M.T.M. Hasanin, and A.M. Abdellah, Synthesis, characterization and cytotoxic evaluation of chitosan nanoparticles: in vitro liver cancer model. Advances in Natural Sciences: Nanoscience and Nanotechnology, 2016. 7: p. 035008.
  25. Lorscheidt, S., M.A. Shetab Boushehri, S. Klaschik, and A. Lamprecht, Sub-cytotoxic doses of pharmaceutical silica nanoparticles show significant impact on the proteome of HepG2 cells. Journal of Controlled Release, 2019. 306: p. 1–14.
  26. Ma, L., Z. Wu, Z. Lu, L. Yan, X. Dong, and Z. Dai, Differences in toxicity induced by various polymer types of nanoplastics on HepG2 cells. Science of the Total Environment, 2024. 918: p. 170664.
  27. International Organization for Standardization, Biological evaluation of medical devices – Part 5: Tests for in vitro cytotoxicity. Geneva, 2009.
  28. Dawlee, S., A. Jayakrishnan, and M. Jayabalan, Studies on novel radiopaque methyl methacrylate–glycidyl methacrylate based polymer for biomedical applications. Journal of Materials Science: Materials in Medicine, 2009. 20: p. 243–250.
  29. Hong, H., O.J. Lee, Y.J. Lee, J.S. Lee, O. Ajiteru, and H. Lee, Cytocompatibility of modified silk fibroin with glycidyl methacrylate for tissue engineering applications. Biomolecules, 2021. 11: p. 35.
  30. Rosillo-De la Torre, A., T. Pérez-Moreno, I.A. Quintero-Ortega, P. Sahare, D.A. Bravo-Alfaro, and H.E. Martínez-Flores, Immunocytochemistry response of a hybrid chitosan-g-glycidyl methacrylate-xanthan scaffold for cell proliferation. RSC Advances, 2025. 15: p. 7693–7708.
  31. Yuan, S., G. Xiong, F. He, W. Jiang, B. Liang, and S. Pehkonen, PCL microspheres tailored with carboxylated poly(glycidyl methacrylate)-REDV conjugates for endothelial cell expansion. Journal of Materials Chemistry B, 2015. 3: p. 8670–8683.
  32. Poplawski, T., E. Pawlowska, M. Wisniewska-Jarosinska, D. Ksiazek, K. Wozniak, and J. Szczepanska, Cytotoxicity and genotoxicity of glycidyl methacrylate. Chemical Biology Interactions, 2009. 180: p. 69–78.
  33. Mihailescu, M., L.C. Miclea, A.M. Pleava, N. Tarba, E.N. Scarlat, and R.D. Negoita, Method for nanoparticle uptake evaluation based on double-labeled fluorescent cells scanned in enhanced darkfield microscopy. Biomedical Optics Express, 2023. 14: p. 2796.
  34. Behzadi, S., V. Serpooshan, W. Tao, M.A. Hamaly, M.Y. Alkawareek, and E.C. Dreaden, Cellular uptake of nanoparticles: journey inside the cell. Chemical Society Reviews, 2017. 46: p. 4218–4244.
  35. Augustine, R., A. Hasan, R. Primavera, R.J. Wilson, A.S. Thakor, and B.D. Kevadiya, Cellular uptake and retention of nanoparticles: insights on particle properties and interaction with cellular components. Materials Today Communications, 2020. 25: p. 101692.
  36. Zhang, J., R. Liu, Z. Yang, C. Luo, J. Chen, and B. Guo, Physicochemical and surface properties of nanoparticles: effects on cellular pathway and uptake. ChemNanoMat, 2025. 11: p. 00043.
  37. Castillo-Aguirre, A.A., B.A. Velásquez-Silva, C. Palacio, F. Baez, Z.J. Rivera-Monroy, and M. Maldonado, Surface modification of poly(GMA-co-EDMA-co-MMA) with resorcarenes. Journal of the Brazilian Chemical Society, 2018. 29: p. 1965–1972.
  38. Çakmak, S., M. Gümüşderelioğlu, and A. Denizli, Biofunctionalization of magnetic poly(glycidyl methacrylate) microspheres with protein A: characterization and cellular interactions. Reactive & Functional Polymers, 2009. 69: p. 586–593.
  39. Fröhlich, E., The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles. International Journal of Nanomedicine, 2012. 7: p. 5577–5591.
  40. Huang, X., W. Xie, X. Yu, C. Fan, J. Wang, and Y. Cao, Methyl-cantharidimide inhibits growth of human hepatocellular carcinoma cells by inducing cell cycle arrest and promoting apoptosis. Frontiers in Oncology, 2019. 9: p. 1234.
  41. Septiadi, D., F. Crippa, T.L. Moore, B. Rothen-Rutishauser, and A. Petri-Fink, Nanoparticle–cell interaction: a cell mechanics perspective. Advanced Materials, 2018. 30: p. 04463.
  42. Miller, M.A. and R. Weissleder, Imaging the pharmacology of nanomaterials by intravital microscopy: toward understanding their biological behavior. Advanced Drug Delivery Reviews, 2017. 113: p. 61–86.
  43. Barbalinardo, M., F. Chiarini, G. Teti, F. Paganelli, E. Mercadelli, and A. Bartoletti, Surface charge overrides protein corona formation in determining cytotoxicity, cellular uptake, and biodistribution of silver nanoparticles. ACS Applied Bio Materials, 2025. 8: p. 5032–5043.
  44. Hoffman, E.G., The battle against quenching and fading for fluorescence: a model of how to evaluate the fluorophore’s behaviour. Journal of Histochemistry and Cytochemistry, 2023. 71: p. 409–410.