SavedAdd your journal

Journals / Çukurova Anestezi ve Cerrahi Bilimler Dergisi / 2024 / Cilt: 7 Sayı: 4

Thymoquinone in a Rat Model of Mesenteric Ischemia-Reperfusion Injury

Gorkem Ozdemir(Eskişehir Osmangazi University, Faculty of Medicine,)0000-0002-2335-1538
Necdet Fatih Yaşar(Eskişehir Osmangazi University, Faculty of Medicine,)0000-0002-9751-2912
Deniz Arık(Eskişehir Osmangazi University, Faculty of Medicine,)0000-0003-0905-2731
İ. Özkan Alataş(Eskişehir Osmangazi University, Faculty of Medicine,)0000-0002-1753-8873
Pages
243–246
Publication Date
2024-12-31
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Pure DMSO and low dose thymoquinone groups showed a similar protective effect; however pure DMSO caused more deterioration in laboratory findings than low dose thymoquinone.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: The objective of this study was to assess the protective efficacy of thymoquinone at low and high doses against ischemic reperfusion (I/R) injury resulting from superior mesenteric artery occlusion in rats.
📊
Key Findings & Data:Materials and Methods: Thirty-five Wistar Albino rats were randomly divided into five groups: control, sham, two thymoquinone treatment groups (50 and 100 mg/kg), and a DMSO control.
💡
Conclusion & Impact:Pure DMSO and low dose thymoquinone groups showed a similar protective effect; however pure DMSO caused more deterioration in laboratory findings than low dose thymoquinone.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:thymoquinone · intestinal ischemia · reperfusion injury.

Abstract

Aim: The objective of this study was to assess the protective efficacy of thymoquinone at low and high doses against ischemic reperfusion (I/R) injury resulting from superior mesenteric artery occlusion in rats. Materials and Methods: Thirty-five Wistar Albino rats were randomly divided into five groups: control, sham, two thymoquinone treatment groups (50 and 100 mg/kg), and a DMSO control. Intestinal injury was assessed using the Park/Chiu classification system. Blood samples were analyzed for liver enzymes (ALT, AST, ALP), kidney function markers (BUN), and other markers (LDH, phosphorus). Results: DMSO and low dose thymoquinone groups showed significantly better Park and Chiu scores (p values were 0.015 and p=0.016 respectively) High-dose thymoquinone and DMSO groups had significantly higher ALT levels than the control. Sham, low-dose thymoquinone, and DMSO groups had significantly higher AST levels than the control. Both low-dose and high-dose thymoquinone groups had significantly higher BUN levels than the control. Conclusion: Our study suggested that low-dose thymoquinone was more effective than high-dose thymoquinone in mitigating I/R-induced intestinal injury. High-dose thymoquinone appeared to have a detrimental effect on intestinal tissue, as evidenced by the lack of significant improvement in histopathological scores compared to the sham group. Conclusion: Our findings suggest that thymoquinone at low doses is more effective in I/R injury than at high doses. Pure DMSO and low dose thymoquinone groups showed a similar protective effect; however pure DMSO caused more deterioration in laboratory findings than low dose thymoquinone.

🔖

Cite This Article

Ozdemir, G., Yaşar, N. F., Arık, D., Alataş, İ. Ö. (2024). Thymoquinone in a Rat Model of Mesenteric Ischemia-Reperfusion Injury. Çukurova Anestezi ve Cerrahi Bilimler Dergisi, Cilt: 7 Sayı: 4, 243–246. https://doi.org/10.36516/jocass.1583178

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (19)

  1. Audu CO, Schechtman DW, Davis FM. Mesenteric Ischemia. Clin Colon Rectal Surg. 2023; 37(6):417-23. https://doi.org/10.1055/s-0043-1777667
  2. u H., Kirkpatrick I.D.C. An Update on Acute Mesenteric Ischemia. Can. Assoc. Radiol. J. 2023; 74(1):160–71. https://doi.org/10.1177/08465371221094280
  3. Gore R.M., Yaghmai V., Thakrar K.H, et al. Imaging in Intestinal Ischemic Disorders. Radiol. Clin. N. Am. 2008; 46(5):845–875. https://doi.org/10.1016/j.rcl.2008.05.004
  4. Corcos O, Nuzzo A. Gastro-intestinal vascular emergencies. Best Pract Res Clin Gastroenterol. 2013; 27(5):709-25. https://doi.org/ 10.1016/j.bpg.2013.08.006
  5. Clair DG, Beach JM. Mesenteric Ischemia. N Engl J Med. 2016; 374(10):959-68. https://doi.org/10.1056/NEJMra1503884
  6. Tiwari G, Gupta M, Devhare LD, et al. Therapeutic and Phytochemical Properties of Thymoquinone Derived from Nigella sativa. Curr Drug Res Rev. 2024; 16(2):145-56. https://doi.org/10.2174/2589977515666230811092410
  7. Gholamnezhad Z, Havakhah S, Boskabady MH. Preclinical and clinical effects of Nigella sativa and its constituent, thymoquinone: A review. J Ethnopharmacol. 2016; 190:372-86. https://doi.org/10.1016/j.jep.2016.06.061
  8. Bulama I, Nasiru S, Bello A, et al. Antioxidant-based neuroprotective effect of dimethylsulfoxide against induced traumatic brain injury in a rats model. Front Pharmacol. 2022; 13:998179. https://doi.org/10.3389/fphar.2022.998179
  9. Elisia I, Nakamura H, Lam V, et al. DMSO Represses Inflammatory Cytokine Production from Human Blood Cells and Reduces Autoimmune Arthritis. PLoS One. 2016;11(3):e0152538. https://doi.org/10.1371/journal.pone.0152538
  10. Parlar A, Arslan SO. Thymoquinone reduces ischemia and reperfusion-induced intestinal injury in rats, through anti-oxidative and anti-inflammatory effects. Turk J Surg. 2020; 36(1): 96-104. https://doi.org/10.5578/turkjsurg.4583
  11. Aydın MS, Kocarslan A, Kocarslan S, et al. Thymoquinone protects end organs from abdominal aorta ischemia/reperfusion injury in a rat model. Rev Bras Cir Cardiovasc. 2015;30(1):77-83. https://doi.org/ 10.5935/1678-9741.20140066
  12. Ong YS, Saiful Yazan L, Ng WK, et al. Acute and subacute toxicity profiles of thymoquinone-loaded nanostructured lipid carrier in BALB/c mice. Int J Nanomedicine. 2016; 11:5905-15. https://doi.org/ 10.2147/IJN.S114205
  13. Tas U, Ayan M, Sogut E, et al. Protective Effects of Thymoquinone and Melatonin on Intestinal Ischemia–reperfusion Injury. Saudi J Gastroenterol. 2015; 21(5): 284-9. https://doi.org/ 10.4103/1319-3767.166203
  14. Flagg Brayton C. Dimethyl Sulfoxide: A review. Cornell Vet. 1986; 76:61-90. 15.Wood DC, Wood J. Pharmacologic and biochemical considerations of dimethyl sulfoxide. Ann N Y Acad Sci. 1975; 243:7-19. https://doi.org/ 10.1111/j.1749-6632.1975.tb25339.x
  15. Jacob SW, Herschler R. Pharmacology of DMSO. Cryobiology. 1986; 23(1):14-27. https://doi.org/10.1016/0011-2240(86)90014-3
  16. He GZ, Zhou KG, Zhang R, et al. Chen, Impact of intestinal ischemia/reperfusion and lymph drainage on distant organs in rats. World J Gastroenterol. 2012; 18: 7271–8. https://doi.org/10.3748/wjg.v18.i48.7271
  17. Schwarz B, Salak N, Hofstötter H, et al. Intestinal ischemic reperfusion syndrome: pathophysiology, clinical significance, therapy. Wiener Klinische Wochenschrift. 1999; 111(14):539-48. PMID: 10467640
  18. Saidi SA, Ncir M, Chaaben R, et al. Liver injury following small intestinal ischemia reperfusion in rats is attenuated by Pistacia lentiscus oil: antioxidant and anti-inflammatory effects. Arch Physiol Biochem. 2017; 123(4):199-205. https://doi.org/ 10.1080/13813455.2017.1302961
  19. Alexandropoulos D, Bazigos GV, Doulamis IP, et al., Protective effects of N-acetylcystein and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion. Biomedicine & Pharmacotherapy. 2017; 89:673–80. https://doi.org/10.1016/j.biopha.2017.02.086