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Journals / Bozok Tıp Dergisi / 2026 / Cilt: 10 Sayı: 2

SERULEİNLE OLUŞTURULMUŞ SIÇAN AKUT PANKREATİT MODELINDE BETA GLUKAN VE FUKOKSANTİNİN ETKİSİ The Effect of Beta Glucan and Fucoxanthine in a Serulein- Induced Acute Pancreatitis in Rats

Dilek Özbeyli(MARMARA ÜNİVERSİTESİ)
Özlem Tuğçe Çilingir Kaya(MARMARA ÜNİVERSİTESİ)
Aslı Aykaç(YAKIN DOĞU ÜNİVERSİTESİ)
Sezgin Aydemir(MARMARA ÜNİVERSİTESİ)
Esra Bihter Gürler(İSTANBUL ATLAS ÜNİVERSİTESİ)
Meral Yüksel(MARMARA ÜNİVERSİTESİ)
Pages
189–197
Publication Date
2020-06-08
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Keywords: Beta glucan; fucoxanthine; COX-2; Oxidative stress

📌 3 Key Takeaways

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Objective & Hypothesis:ÖZET Amaç: Bu çalışmada, Beta Glukan, fukoksantin ve kombinasyonlarının serulein kaynaklı akut pankreatit (AP) sıçan modelindeki etkileri araştırıldı.
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Key Findings & Data:AP bir saat arayla dört kez serulein (20 ug/kg/ ip) enjeksiyonuyla oluşturuldu.
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Conclusion & Impact:Keywords: Beta glucan; fucoxanthine; COX-2; Oxidative stress
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:SERULEİNLE OLUŞTURULMUŞ SIÇAN AKUT PANKREATİT MODELINDE BETA GLUKAN VE FUKOKSANTİNİN ETKİSİ The Effect of Beta Glucan and Fucoxanthine in a Serulein- Induced Acute Pancreatitis in Rats

Özet

ÖZET Amaç: Bu çalışmada, Beta Glukan, fukoksantin ve kombinasyonlarının serulein kaynaklı akut pankreatit (AP) sıçan modelindeki etkileri araştırıldı. Gereç ve Yöntem: Sıçanlar kontrol ve AP gruplarına ayrıldı. AP bir saat arayla dört kez serulein (20 ug/kg/ ip) enjeksiyonuyla oluşturuldu. Serulein enjeksiyonundan 3 gün önce AP gruplarına günde 1 kez taşıyıcı, Beta Glukan (BG;40 mg/kg/po), fukoksantin (Fuko;40 mg/kg/po) veya Beta Glukan (BG;40 mg/kg/po) +fukoksantin (Fuko;40 mg/kg/po) uygulandı. Son enjeksiyondan sonraki 8. saatte ötenazi gerçekleştirildi. Serumda amilaz ve lipaz, pankreas dokusunda interlökin-1β (IL-1β), interlökin-6 (IL-6), interlökin-10 (IL- 10), lucigenin kemilüminesans (CL), malondialdehit (MDA), miyeloperoksidaz (MPO) enzim aktivitesi ve Hematoksilen&Eosin (H&E), Siklooksijenaz-2 (COX-2), nükleer faktör kappa B (NF-κB) histopatolojik incelemeleri yapıldı. İstatistiksel analizler için tek yönlü ANOVA ve Bonferroni post-hoc testi uygulandı. Bulgular: Taşıyıcı grubunda; amilaz, lipaz, lusigenin, IL-1β ve IL-6 düzeyleri kontrole göre (p <0,01-0,0001) yükselirken, GSH ve IL-10 düzeyleri azaldı (p<0,0001). Lipaz ve amilaz düzeyleri; BG, Fuko ve BG+Fuko tedavileri ile taşıyıcıya göre azaldı (p<0,05-0,001). IL-6 düzeyleri, Fuko ve BG+Fuko grubunda taşıyıcıya göre azaldı (p <0,01). IL-1β düzeyleri; BG, Fuko ve BG+Fuko gruplarında taşıyıcı grubuna göre azaldı (p<0,001- 0.0001). IL-10 düzeyi yalnızca BG grubunda taşıyıcı grubuna göre arttı (p<0,01). H&E, COX-2 skorları taşıyıcı grubunda kontrole göre artarken (p<0,0001); BG, Fuko ve BG+Fuko tedavileri ile azalma gözlendi (p<0,05- 0,001). Taşıyıcı grubunda artan NF-κB skorları (p<0,0001), BG tedavisi ile azaldı (p<0,01). Taşıyıcı grubundaki artan lusigenin kemiluminesans, MPO ve MDA düzeyleri (p <0,01-0,0001) tüm tedavilerle azaldı (p <0,01- 0,0001). Sonuç: Beta Glukan ve fukoksantin tedavisi pro-inflamatuvar sitokinleri, COX-2 düzeyini ve oksidatif belirteçleri azaltarak anti-inflamatuvar sitokin düzeyini arttırarak AP gelişimini hafifletmiştir. Anahtar sözcükler: Beta glukan; fukoksantin; COX-2; oksidatif stres ABSTRACT Aim: This study aims to investigate the effects of Beta Glucan, fucoxanthin and their combinations in acute pancreatitis (AP) rat model. Material and Methods: Rats were divided into control and AP groups. The AP was induced by injection of cerulein (20 μg/kg/ip) four times with an hour interval. Vehicle, Beta Glucan (BG;40 mg/kg/po), fucoxanthin (Fuco;40 mg/kg/po) or Beta Glucan (BG;40 mg/kg/+ fucoxanthin (Fuco;40 mg/kg/po) were administered 3 days before cerulein injection. Euthanasia was performed at the 8th hour after the last injection. Serum and pancreatic tissue collected for biochemical and histopathological examinations. One-way ANOVA and Bonferroni post-hoc test was used for statistical analysis. Results: Amylase, lipase, lucigenin, interleukin-1β, and interleukin-6 levels are significantly elevated in the vehicle-treated AP groups (p<0.01-0.0001) while IL-10 was decreased (p<0.0001). Lipase and amylase levels decreased in the BG, Fuco and BG+Fuco groups compared to vehicle group (p<0.05-0.001). IL-6 levels, decreased in the fuco and BG+Fuco group (p <0.01). IL-1β levels decreased in BG, Fuco and BG+Fuco groups compared to vehicle group (p<0,001-0.0001). IL-10 levels increased in BG group compared to the vehicle group (p<0.01). H&E and COX-2 scores increased in the vehicle groups, compared to the control group (p<0.0001). All treatments decreased that scores (p<0.05-0.001). Increased NF-κB scores with vehicle (p<0.0001), decreased with BG treatment (p<0.01). Increased lucigenin, MPO and MDA levels (p<0.01- 0.0001), decreased with BG, Fuco and BG+Fuco treatments (p<0.01-0.0001). Conclusion: Treatment of Beta Glucan and fucoxanthin alleviated AP development by decreasing proinflammatory cytokines, COX-2 and oxidative markers and increasing anti-inflammatory cytokine. Keywords: Beta glucan; fucoxanthine; COX-2; Oxidative stress

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Cite This Article

Özbeyli, D., Kaya, Ö. T. Ç., Aykaç, A., Aydemir, S., Gürler, E. B., Yüksel, M. (2020). SERULEİNLE OLUŞTURULMUŞ SIÇAN AKUT PANKREATİT MODELINDE BETA GLUKAN VE FUKOKSANTİNİN ETKİSİ The Effect of Beta Glucan and Fucoxanthine in a Serulein- Induced Acute Pancreatitis in Rats. Bozok Tıp Dergisi, Cilt: 10 Sayı: 2, 189–197. https://doi.org/10.16919/bozoktip.710744

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