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Journals / Experimed / 2022 / Volume: 12 Issue: 3

The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress

Sümeyye Yılmaz(FENERBAHÇE ÜNİVERSİTESİ, SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU)0000-0001-5529-7380
Elif Tufan(MARMARA UNIVERSITY)0000-0003-0684-3693
Güzin Göksun Sivas(MARMARA UNIVERSITY)0000-0001-7347-490X
Begüm Gürel Gökmen(MARMARA UNIVERSITY)0000-0002-3955-1948
Ercan Dursun(MARMARA UNIVERSITY)0000-0001-6025-9565
Dilek Özbeyli(MARMARA UNIVERSITY)0000-0002-4141-6913
Göksel Şener(FENERBAHCE UNIVERSITY)0000-0001-7444-6193
Tuğba Tunalı-akbay(MARMARA UNIVERSITY)0000-0002-2091-9298
Journal
Experimed
Pages
—
Publication Date
2022-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage.
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Key Findings & Data:Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey.
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Conclusion & Impact:Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Methotrexate · whey protein · brain

Abstract

Objectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage. Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-S-transferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out. Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTX-induced oxidative stress. MTX administration did not change the brain’s NO level, while an increase in intestinal NO level was detected. Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage.

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Cite This Article

Yılmaz, S., Tufan, E., Sivas, G. G., Gökmen, B. G., Dursun, E., Özbeyli, D., Şener, G., Tunalı-akbay, T. (2022). The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress. Experimed, Volume: 12 Issue: 3. https://doi.org/10.26650/experimed.1189898

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