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Journals / European Journal of Biology / 2026 / Volume: 84 Issue: 1

Protective Effects of Cuscuta sp. against Cardiorenal Injury in Bile Duct-Ligated Rats

Bilge Nur Hatipoğlu(MARMARA UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PROFESSIONAL PHARMACEUTICAL SCIENCES, DEPARTMENT OF PHARMACOLOGY)0000-0001-5420-8681
Dilek Özbeyli(MARMARA UNIVERSITY, HEALTH SERVICES VOCATIONAL SCHOOL, DEPARTMENT OF MEDICAL SERVICES AND TECHNIQUES, MEDICAL IMAGING TECHNIQUES PR.)0000-0002-4141-6913
Ali Şen(MARMARA UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PROFESSIONAL PHARMACEUTICAL SCIENCES, DEPARTMENT OF PHARMACOGNOSY)0000-0002-2144-5741
Özge Çevik(ADNAN MENDERES UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF MEDICAL BIOCHEMISTRY)0000-0002-9325-3757
Feriha Ercan(MARMARA UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0003-2339-5669
Ömercan Albayrak(Anatolia Geneworks, Department of Research and Development)0000-0002-6254-3786
Seren Ede Pazarbaşı(Fenerbahçe University, Vocational School of Health Services, Pharmacy Services Program)0000-0002-3195-4064
Fatma Kanpalta Mustafaoğlu(MARMARA UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF HISTOLOGY AND EMBRYOLOGY)0000-0001-9832-6938
Göksel Şener(Fenerbahçe University, Faculty of Pharmacy, Department of Pharmacology)0000-0001-7444-6193
Pages
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Publication Date
2025-06-05
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: CUS has protective effects against oxidative damage and offers antioxidant and anti-inflammatory benefits against cholestasis-induced tissue injury.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Bile duct ligation (BDL) obstructs bile flow, resulting in bile and toxic substances buildup that causes liver damage.
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Key Findings & Data:The C and BDL groups received saline, whereas the other groups received oral 250 mg/kg CUS.
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Conclusion & Impact:Conclusion: CUS has protective effects against oxidative damage and offers antioxidant and anti-inflammatory benefits against cholestasis-induced tissue injury.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Bile duct ligation · Fibrosis · Cardiorenal injury

Abstract

Objective: Bile duct ligation (BDL) obstructs bile flow, resulting in bile and toxic substances buildup that causes liver damage. This study investigated the protective effects of Cuscuta sp. methanol extract (CUS) against cardiorenal injury in bile duct-ligated rats. Materials and Methods: Rats were categorised into four groups: Control (C), CUS, BDL, and BDL+CUS. The C and BDL groups received saline, whereas the other groups received oral 250 mg/kg CUS. After 28 days, blood, kidney, and heart tissue samples were collected for biochemical and histological analyses. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), direct bilirubin (DB), and total bilirubin (TB) levels were analysed to determine liver function, while Transforming growth factor-beta (TGF-β) and hydroxyproline (HYP) levels were evaluated for fibrosis, and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels alongside Na⁺/K⁺-ATPase activity were analysed to assess oxidative stress and membrane injury in the heart and renal tissues. Results: AST, ALT, DB, and TB levels were significantly elevated in the BDL group compared with the C group; however, the levels were distinctly lower in the BDL+CUS group than in the BDL group. Additionally, in both tissues, TGF-β, HYP, and 8-OHdG levels were higher in the BDL group than in the C group, but decreased in the BDL+CUS group, with Na⁺/K⁺-ATPase activity being lower in BDL group compared with the C group and significantly increased in BDL+CUS group. Conclusion: CUS has protective effects against oxidative damage and offers antioxidant and anti-inflammatory benefits against cholestasis-induced tissue injury.

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Cite This Article

Hatipoğlu, B. N., Özbeyli, D., Şen, A., Çevik, Ö., Ercan, F., Albayrak, Ö., Pazarbaşı, S. E., Mustafaoğlu, F. K., Şener, G. (2025). Protective Effects of Cuscuta sp. against Cardiorenal Injury in Bile Duct-Ligated Rats. European Journal of Biology, Volume: 84 Issue: 1. https://doi.org/10.26650/EurJBiol.2025.1568086

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References (43)

  1. Gedik N, KabasakaL L, SehirLi O, et aL. Long-term administration of aqueous garLic extract (AGE) aLLeviates Liver fibrosis and oxidative damage induced by biLiary obstruction in rats. Life Sci. 2005;76(22):2593-2606. google scholar
  2. OreLLana M, Rodrigo R, ThieLemann L, Guajardo V. BiLe duct Ligation and oxidative stress in the rat: effects in Liver and kidney. Comp Biochem Physiol C Toxicol Pharmacol. 2000;126(2):105-111. google scholar
  3. SokoL RJ, WinkLhofer-Roob BM, Devereaux MW, McKim JM Jr. Generation of hydroperoxides in isoLated rat hepatocytes and hepatic mitochondria exposed to hydrophobic biLe acids. Gastroenterology. 1995;109(4):1249-1256. google scholar
  4. Maher JJ, Friedman SL. ParenchymaL and nonparenchymaL ceLL interactions in the Liver. Semin Liver Dis. 1993;13(1):13-20. google scholar
  5. ALcoLado R, Arthur MJ, IredaLe JP. Pathogenesis of Liver fibrosis. Clin Sci (Lond). 1997;92(2):103-112. google scholar
  6. Zhou WC, Zhang QB, Qiao L. Pathogenesis of liver cirrhosis. World J Gastroenterol. 2014;20(23):7312-7324. google scholar
  7. Bolognesi M, Di Pascoli M, Verardo A, Gatta A. Splanchnic vasodilation and hyperdynamic circulatory syndrome in cirrhosis. World J Gastroenterol. 2014;20(10):2555-2563. google scholar
  8. Pereira RM, dos Santos RA, Oliveira EA, et al. Development of hepatorenal syndrome in bile duct ligated rats. World J Gastroenterol. 2008;14(28):4505-4511. Yang YY, Lin HC. The heart: pathophysiology and clinical implications of cirrhotic cardiomyopathy. J Chin Med Assoc. 2012;75(12):619-623. google scholar
  9. Gupta K, Bhurwal A, Law C, et al. Acute kidney injury and hepatorenal syndrome in cirrhosis. World J Gastroenterol. 2021;27(26):3984-4003. google scholar
  10. Sola E, Gines P. Challenges and Management of Liver Cirrhosis: Pathophysiology of Renal Dysfunction in Cirrhosis. Dig Dis. 2015;33(4):534-538. google scholar
  11. Bernardi M, Moreau R, Angeli P, Schnabl B, Arroyo V, Mechanisms of decompensation and organ failure in cirrhosis: From peripheral arterial vasodilation to systemic inflammation hypothesis, J Hepatol, 2015;63(5):1272-1284, google scholar
  12. M0ller S, Henriksen JH, Cirrhotic cardiomyopathy, J Hepatol. 2010;53(l):179-190. Carvalho MVH, Kroll PC, Kroll RTM, Carvalho VN, Cirrhotic cardiomyopathy: the liver affects the heart, Braz J Med Biol Res, 2O19;52(2):e78O9, google scholar
  13. Kawase A, Kazaoka A, Yamamoto R, Minakata R, Shimada H, Iwaki M, Changes in transporters and metabolizing enzymes in the livers of rats with bile duct ligation, J Pharm Pharm Sci, 2O19;22(1):457-465, google scholar
  14. Kountouras J, Billing BH, Scheuer PJ, Prolonged bile duct obstruction: a new experimental model for cirrhosis in the rat, Br J Exp Pathol, 1984;65(3):3O5-311, Sener G, Kabasakal L, Sehirli O, Ercan F, Gedik N, 2-Mercaptoethane sulfonate (MESNA) protects against biliary obstruction-induced oxidative damage in rats, Hepatol Res, 2OO6;35(2):14O-146, google scholar
  15. Sener G, Sehirli AO, Toklu HZ, Yuksel M, Ercan F, Gedik N, Erdosteine treatment attenuates oxidative stress and fibrosis in experimental biliary obstruction, Pediatr Surg Int, 2OO7;23(3):233-241, google scholar
  16. Peres W, Tunon MJ, Collado PS, Herrmann S, Marroni N, Gonzalez-Gallego J,The flavonoid quercetin ameliorates liver damage in rats with biliary obstruction, J Hepatol, 2OOO;33(5):742-75O, google scholar
  17. Padillo FJ, Muntane J, Montero JL, et al, Effect of internal biliary drainage on plasma levels of endotoxin, cytokines, and C-reactive protein in patients with obstructive jaundice, World J Surg, 2OO2;26(11):1328-1332, google scholar
  18. Toklu HZ, Dumlu MU, Sehirli O, et al, Pomegranate peel extract prevents liver fibrosis in biliary-obstructed rats, J Pharm Pharmacol, 2OO7;59(9):1287-1295, Ahmad A, Tandon S, Xuan TD, Nooreen Z, A Review on phytoconstituents and biological activities of Cuscuta species, Biomed Pharmacother, 2O17;92:772-795, Şekeroğlu N, Koca U, Meraler SA, Geleneksel bir halk ilacı: İkşut, Yuzuncu Yıl University, J Agricultural Sci, 2O12: 22(1):235-243, google scholar
  19. Koca-Caliskan U, Yilmaz I, Taslidere A, Yalcin FN, Aka C, Sekeroglu N, Cuscuta arvensis Beyr "Dodder": In Vivo Hepatoprotective effects against Acetaminophen-induced hepatotoxicity in rats, J Med Food. 2O18;21(6):625-631, Criado M, Flores O, Ortız MC, et al, Elevated glomerular and blood mononuclear lymphocyte nitric oxide production in rats with chronic bile duct ligation: Role of inducible nitric oxide synthase activation, Hepatology, 1997;26(2):268-276, Sener G, Toklu H, Kapucu C, et al, Melatonin protects against oxidative organ injury in a rat model of sepsis, Surg Today, 2OO5;35(1):52-59, google scholar
  20. Acikel Elmas M, Cakıcı SE, Dur IR, et al, Protective effects of exercise on heart and aorta in high-fat diet-induced obese rats, Tissue Cell, 2O19;57:57-65, google scholar
  21. Sasso R, Abou Yassine A, Deeb L, Predictors of development of hepatorenal syndrome in hospitalized cirrhotic patients with acute kidney injury, J Clin Med, 2O21;1O(23):5621, doi:1O,339O/jcm1O235621 google scholar
  22. Mousavi K, Niknahad H, Ghalamfarsa A, et al, Taurine mitigates cirrhosis-associated heart injury through mitochondrial-dependent and antioxidative mechanisms, Clin Exp Hepatol, 2O2O;6(3):2O7-219, google scholar
  23. Møller S, Lee SS, Cirrhotic cardiomyopathy. J Hepatol. 2018;69(4):958-960. google scholar
  24. Liu L, Liu H, Nam SW, Lee SS, Protective effects of erythropoietin on cirrhotic cardiomyopathy in rats, Dig Liver Dis, 2O12;44(12):1O12-1O17, google scholar
  25. Kazory A, Ronco C, Hepatorenal syndrome or hepatocardiorenal syndrome: Revisiting basic concepts in view of emerging data, Cardiorenal Med, 2O19;9(1):1-7, google scholar
  26. Matyas C, Erdelyi K, Trojnar E, et al, Interplay of liver-heart inflammatory axis and cannabinoid 2 receptor signaling in an experimental model of hepatic cardiomyopathy, Hepatology, 2O2O;71(4):1391-14O7, google scholar
  27. Liao JC, Chang WT, Lee MS, et al, Antinociceptive and anti-inflammatory activities of Cuscuta chinensis seeds in mice, Am J Chin Med. 2O14;42(1):223-242, google scholar
  28. Donnapee S, Li J, Yang X, et al, Cuscuta chinensis Lam,: A systematic review on ethnopharmacology, phytochemistry and pharmacology of an important traditional herbal medicine, J Ethnopharmacol. 2O14;157:292-3O8, google scholar
  29. Lin MK, Lee MS, Huang HC, Cheng TJ, Cheng YD, Wu CR, Cuscuta chinensis and C. campestris attenuate scopolamine-induced memory deficit and oxidative damage in mice, Molecules. 2O18;23(12):3O6O, doi:1O,339O/molecules23123O6O google scholar
  30. Kim KP, Williams CE, Lemmon CA, Cell-matrix interactions in renal fibrosis, Kidney Dial. 2O22;2(4):6O7-624, google scholar
  31. Liu SY, Huang CC, Huang SF, et al, Pioglitazone ameliorates acute endotoxemia-induced acute on chronic renal dysfunction in cirrhotic ascitic rats, Cells. 2O21;1O(11):3O44,doi:1O,339O/cells1O113O44 google scholar
  32. Toklu HZ, Sehirli O, Erşahin M, et al, Resveratrol improves cardiovascular function and reduces oxidative organ damage in the renal, cardiovascular and cerebral tissues of two-kidney, one-clip hypertensive rats, J Pharm Pharmacol. 2O1O;62(12):1784-1793, google scholar
  33. Shin S, Lee YJ, Kim EJ, Lee AS, Kang DG, Lee HS, Effect of Cuscuta chinensis on renal function in ischemia/reperfusion-induced acute renal failure rats, Am J Chin Med. 2O11;39(5):889-9O2, google scholar
  34. Huang YT, Hsu YC, Chen CJ, Liu CT, Wei YH, Oxidative-stress-related changes in the livers of bile-duct-ligated rats, J Biomed Sci. 2OO3;1O(2):17O-178, google scholar
  35. Matono T, Koda M, Tokunaga S, Sugihara T, Ueki M, Murawaki Y, The effects of the selective mineralocorticoid receptor antagonist eplerenone on hepatic fibrosis induced by bile duct ligation in rat, Int J Mol Med. 2O1O;25(6):875-882, google scholar
  36. Chen KH, Chou YC, Hsiao CY, et al, Amelioration of serum 8-OHdG level by enzyme replacement therapy in patients with Fabry cardiomyopathy, Biochem Biophys Res Commun. 2O17;486(2):293-299, google scholar
  37. Heidari R, Mandegani L, Ghanbarinejad V, et al, Mitochondrial dysfunction as a mechanism involved in the pathogenesis of cirrhosis-associated cholemic nephropathy, Biomed Pharmacother. 2O19;1O9:271-28O, google scholar
  38. Sheibani M, Nezamoleslami S, Mousavi SE, et al, Protective effects of spermidine against cirrhotic cardiomyopathy in bile duct-ligated rats, J Cardiovasc Pharmacol, 2O2O;76(3):286-295, google scholar Albayrak Ö, Özbeyli D, Şen A, et al, Dodder (Cuscuta sp,) extract ameliorates liver fibrosis in bile duct-ligated rats, Eur J Biol, 2O22;81(1):31-4O, google scholar
  39. Razavi-Azarkhiavi K, Iranshahy M, Sahebkar A, Shirani K, Karimi G, The protective role of phenolic compounds against doxorubicin-induced cardiotoxicity: A comprehensive review, Nutr Cancer. 2O16;68(6):892-917, google scholar Birdane YO, Atik H, Atik O, Aslan R, Mandarin peel ethanolic extract attenuates diclofenac sodium induced hepatorenal toxicity in rats by mitigating oxidative stress and inflammation, Drug Chem Toxicol. 2O24;47(2):18O-19O, google scholar Sief MM, Khalil FA, Abou-Arab AA, et al, Ameliorative role of Melissa officinalis against hepatorenal toxicities of organophosphorus malathion in male rats, MOJ Toxıcol. 2015;l(3):103-109. google scholar
  40. Othman MS, Khaled AM, Al-Bagawi AH, et al, Hepatorenal protective efficacy of flavonoids from Ocimum basilicum extract in diabetic albino rats: A focus on hypoglycemic, antioxidant, anti-inflammatory and anti-apoptotic activities. Biomed Pharmacother. 2021;144:112287, doi:10,1016/j,biopha,2021,112287 google scholar
  41. Guo W, Long X, Lv M, Deng S, Liu D, Yang Q, Effect of thymoquinone on sepsis-induced cardiac damage via anti-inflammatory and anti-apoptotic mechanisms, J Int Med Res, 2022;50(9):3000605221118680, doi:10,1177/03000605221118680 google scholar
  42. Gelen V, Şengül E, Antioxidant, anti-inflammatory and antiapoptotic effects of Naringin on cardiac damage induced by cisplatin, Indian J Tradit Knowl. 2020;19(2):459-465, google scholar
  43. Menon S, Lawrence L, Sivaram VP, Padikkala J. Oroxylum indicum root bark extract prevents doxorubicin-induced cardiac damage by restoring redox balance. J Ayurveda Integr Med. 2019;10(3):159-165. google scholar