SavedAdd your journal

Journals / European Journal of Biology / 2024 / Volume: 83 Issue: 2

Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats

Dilek Özbeyli(Marmara University, Vocational School of Health Services, Department of Medical Services and Techniques)0000-0002-4141-6913
Ali Şen(Marmara University, Faculty of Pharmacy, Department of Pharmacognosy)0000-0002-2144-5741
Naziye Özkan Yenal(Marmara University, Vocational School of Health Services, Department of Pathology Laboratory Techniques)0000-0003-3524-0672
Ayşe Nur Hazar-yavuz(Marmara University, Faculty of Pharmacy, Department of Pharmacology)0000-0003-0784-8779
Deniz Mukaddes Türet(Aziz Sancar Institute of Experimental Medicine, Department of Laboratory Animals Science, Istanbul University)0000-0003-1989-9637
Meral Yüksel(Marmara University, Vocational School of Health Services, Department of Medical Laboratory Techniques)0000-0002-4760-3306
Leyla Bitiş(Marmara University, Faculty of Pharmacy, Department of Pharmacognosy)0000-0003-1167-6666
Ahmet Doğan(Marmara University, Faculty of Pharmacy, Department of Pharmaceutic Botanic)0000-0003-0603-5100
Aslı Aykaç(Near East University, Vocaional School of Health Services, Department of Biophysics)0000-0002-4885-5070
Pages
—
Publication Date
2024-12-19
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our findings support the ethnopharmacological use of SP for colitis.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: This study evaluated the antioxidant and anti-inflammatory effects of Sideritis perfoliata (SP) ethanolic extract in vitro and its impact on anti-inflammatory, antiapoptotic, oxidative stress, and tissue damage-related parameters in an ulcerative colitis (UC) rat model.
📊
Key Findings & Data:Materials and Methods: Dried and ground aerial parts of SP were extracted with 90% ethanol using maceration.
💡
Conclusion & Impact:Our findings support the ethnopharmacological use of SP for colitis.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Sideritis perfoliata · Ulcerative colitis · Cytokines

Abstract

Objective: This study evaluated the antioxidant and anti-inflammatory effects of Sideritis perfoliata (SP) ethanolic extract in vitro and its impact on anti-inflammatory, antiapoptotic, oxidative stress, and tissue damage-related parameters in an ulcerative colitis (UC) rat model. Materials and Methods: Dried and ground aerial parts of SP were extracted with 90% ethanol using maceration. Then, the SP extract’s radical scavenging, anti-5-lipoxygenase (LOX) activities, and total secondary metabolic quantities were evaluated in vitro. In in vivo experiments, following acetic acid (AA)-induced colitis, physiological saline (PS) (1 mL, og), sulfasalazine (SS) (100 mg/kg bwt, 1mL, og), and SP extract (200 mg/kg bwt, 1mL, og) treatments were administered to AA, SS, and SP groups, respectively, once daily for three consecutive days. PS was given to the control group. At the 72nd hour, the rats were euthanized. Analyses were performed on colon tissues using ELISA, Chemiluminescence assay, Haematoxylin and Eosin staining. Results: SP extract exhibited good antioxidant activity against 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and strong anti-5-LOX activity. The total amounts of phenol, flavonoid, and triterpene compounds in the extract were determined. Compared with the AA group, SS or SP extract treatments reduced tumour necrosis factor-α, interleukin-1β, interleukin-17, tool-like receptor-9, matrix metalloproteinases-3, caspase-9, caspase-3, luminol, and lucigenin chemiluminescence levels, macroscopic and microscopic morphological scores in colon. Conclusion: Due to its flavonoids, phenolic acids, triterpene content, anti-5-LOX, and free radical scavenging activities, SP extract attenuates proinflammatory cytokines, mitochondrial apoptotic caspases, and oxidative stress. Our findings support the ethnopharmacological use of SP for colitis.

🔖

Cite This Article

Özbeyli, D., Şen, A., Yenal, N. Ö., Hazar-yavuz, A. N., Türet, D. M., Yüksel, M., Bitiş, L., Doğan, A., Aykaç, A. (2024). Sideritis perfoliata Ethanolic Extract Mitigates Acetic Acid-Induced Ulcerative Colitis in Rats. European Journal of Biology, Volume: 83 Issue: 2. https://doi.org/10.26650/EurJBiol.2024.1550119

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (65)

  1. Bruner LP, White AM, Proksell S. Inflammatory Bowel Disease. Prim Care. 2023;50(3):411-427. google scholar
  2. Podolsky DK. Inflammatory bowel disease. N Engl J Med. 2002;347(6):417-429. google scholar
  3. Agrawal M, Jess T. Implications of the changing epidemiol-ogy of inflammatory bowel disease in a changing world. United European Gastroenterol J. 2022;10(10):1113-1120. google scholar
  4. Hilsden RJ, Verhoef MJ, Best A, Pocobelli G. Complementary and alternative medicine use by Canadian patients with inflammatory bowel disease: Results from a national survey. Am J Gastroenterol. 2003;98(7):1563-1568. google scholar
  5. Bensoussan M, Jovenin N, Garcia B, et al. Complementary and alternative medicine use by patients with inflammatory bowel disease: Results from a postal survey. Gastroenterol Clin Biol. 2006;30(1):14-23. google scholar
  6. Dossett ML, Davis RB, Lembo AJ, Yeh GY. Complementary and alternative medicine use by US adults with gastrointestinal con-ditions: Results from the 2012 National Health Interview Survey. Am J Gastroenterol. 2014;109(11):1705-1711. google scholar
  7. Rahimi R, Shams-Ardekani MR, Abdollahi M. A review of the efficacy of traditional Iranian medicine for inflammatory bowel disease. World J Gastroenterol. 2010;16(36):4504-4514. google scholar
  8. Ke F, Yadav PK, Ju LZ. Herbal medicine in the treatment of ulcerative colitis. Saudi J Gastroenterol. 2012;18(1):3-10. google scholar
  9. Magrone T, Perez de Heredia F, Jirillo E, et al. Functional foods and nutraceuticals as therapeutic tools for the treatment of diet-related diseases. Can J Physiol Pharmacol. 2013;91(6):387-396. google scholar
  10. Duda-Chodak A, Tarko T. Possible side effects of polyphenols and their interactions with medicines. Molecules. 2023;28(6):2536. doi: 10.3390/molecules28062536 google scholar
  11. Thorsteinsdottir S, Gudjonsson T, Nielsen OH, Vainer B, Seidelin JB. Pathogenesis and biomarkers of carcinogenesis in ulcerative colitis. Nat Rev Gastroenterol Hepatol. 2011;8(7):395-404. google scholar
  12. Nagib MM, Tadros MG, ElSayed MI, Khalifa AE. Anti-inflammatory and antioxidant activities of olmesartan medoxomil ameliorate experimental colitis in rats. Toxicol Appl Pharmacol. 2013;271(1):106-113. google scholar
  13. Tian T, Wang Z, Zhang J. Pathomechanisms of oxida-tive stress in inflammatory bowel disease and potential an-tioxidant therapies. Oxid Med Cell Longev. 2017;4535194. doi:10.1155/2017/4535194 google scholar
  14. Jena G, Trivedi PP, Sandala B. Oxidative stress in ulcerative colitis: An old concept but a new concern. Free Radic Res. 2012;46(11):1339-1345. google scholar
  15. Kang L, Fang X, Song YH, et al. Neutrophil-epithelial crosstalk during intestinal inflammation. Cell Mol Gastroenterol Hepatol. 2022;14(6):1257-1267. google scholar
  16. Loğoğlu E, Arslan S, Oktemer A, Saköyan I. Biological activ-ities of some natural compounds from Sideritis sipylea Boiss. Phytother Res. 2006;20(4):294-297. google scholar
  17. Krgovic N, Jovanovic M, Aradski AA, et al. Bioassay-guided skin-beneficial effects of fractionated Sideritis raeseri subsp. raeseri Extract. Plants (Basel). 2022;11(20):2677-2692. google scholar
  18. Bizim Bitkiler. Website. https://bizimbitkiler.org.tr/v2/hiyerarsi. php?c=Sideritis. Accessed October 09, 2024. google scholar
  19. Gonzalez-Burgos E, Carretero ME, Gomez-Serranillos MP. Sideritis spp.: Uses, chemical composition and pharmacological activities-A review. J Ethnopharmacol. 2011;135(2):209-225. google scholar
  20. Charami MT, Lazari D, Karioti A, et al. Antioxidant and anti-inflammatory activities of Sideritis perfoliata subsp. perfoliata (Lamiaceae). Phytother Res. 2008;22(4):450-454. google scholar
  21. Todorova M, Trendafilova A. Sideritis scardica Griseb., an en-demic species of Balkan peninsula: Traditional uses, cultivation, chemical composition, biological activity. J Ethnopharmacol. 2014;152(2):256-265. google scholar
  22. Aneva I, Zhelev P, Kozuharova E, et al. Genus Sideritis, sec-tion Empedoclia in southeastern Europe and Turkey-studies in ethnopharmacology and recent progress of biological activities. Daru. 2019;27(1):407-421. google scholar
  23. Lall N, Chrysargyris A, Lambrechts I, et al. Sideritis perfoliata (Subsp. Perfoliata) nutritive value and its potential medicinal properties. Antioxidants (Basel). 2019;8(11):521-538. google scholar
  24. Sahin T, Kılıç Ö, Taşkın T. Ethnobotanical and bioactive charac-teristics of some Sideritis L. Taxa and major essential oil com-pounds. Russ J Biol Res. 2023;10(1):17-29. google scholar
  25. Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochem Anal. 2002;13(1):8-17. google scholar
  26. Sezik E, Ezer N, Huesorodriguez JA, Rodriguez B. Ent-2-alpha-hydroxy-13-epi-manoyl oxide from Sideritis perfoliata. Phytochem. 1985;24:2739-2740. google scholar
  27. Ezer N, Sakar MK, Rodriguez B, De La Torre MC. Flavonoid gly-cosides and a phenylpropanoid glycoside from Sideritis perfoliata. Int J Pharmacogn. 1992;30:61-65. google scholar
  28. Zou Y, Chang SK, Gu Y, Qian SY. Antioxidant activity and phe-nolic compositions of lentil (Lens culinaris var. Morton) extract and its fractions. J Agric Food Chem. 2011;59(6):2268-2276. google scholar
  29. Phosrithong N, Nuchtavorn N. Antioxidant and anti-inflammatory activites of Clerodendrum leaf extracts collected in Thailand. Eur J Integr Med. 2016;8(3):281-285. google scholar
  30. Gao X, Ohlander M, Jeppsson N, Björk L, Trajkovski V. Changes in antioxidant effects and their relationship to phytonutrients in fruits of sea buckthorn (Hippophae rhamnoides L.) during matu-ration. J Agric Food Chem. 2020;48:1485-1490. google scholar
  31. Yıldırım A, Şen A, Doğan A, Bitiş L. Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson. Int J Second Metab. 2019;6:211-222. google scholar
  32. Zhang R, Zeng Q, Deng Y, et al. Phenolic profiles and antioxidant activity of litchi pulp of different cultivars cultivated in Southern China. Food Chem. 2013;136(3-4):1169-1176. google scholar
  33. Chang CL, Lin CS, Lai GH. Phytochemical characteristics, free radical scavenging activities, and neuroprotection of five medicinal plant extracts. Evid Based Complement Alternat Med. 2012;2012:984295. doi:10.1155/2012/984295 google scholar
  34. Balkan A, Balkan M. Legal requirements involving ethics in ani-mal research, laboratory standardisation, and animal care. Thorac Res Pract. 2013;14(Supplement 2):6-9. google scholar
  35. Jaafar FR, Abu-Raghif A. Comparative treatment of Sul-fasalazine+Ezetimibe combination and Sulfasalazine in a rat model with induced colitis. J Med Life. 2023;16(8):1165-1169. google scholar
  36. Tadic VM, Jeremic I, Dobric S, et al. Anti-inflammatory, gas-troprotective, and cytotoxic effects of Sideritis scardica extracts. Planta Med. 2012;78(5):415-427. google scholar
  37. MacPherson BR, Pfeiffer CJ. Experimental production of diffuse colitis in rats. Digestion. 1978;17(2):135-150. google scholar
  38. Karakoyun B, Ertaş B, Yüksel M, et al. Ameliorative effects of riboflavin on acetic acid-induced colonic injury in rats. Clin Exp Pharmacol Physiol. 2018;45:563-572. google scholar
  39. Özbeyli D, Şen A, Aykaç A, et al. Therapeutic effects of Momordica charantia L. ethanolic extract on acetic acid-induced ulcerative colitis in rats. Eur J Biol. 2021;80(2):119-128. google scholar
  40. Haklar G, Ulukaya-Durakbaşa C, Yüksel M, Dağli T, Yalçin AS. Oxygen radicals and nitric oxide in rat mesenteric ischaemia-reperfusion: modulation by L-arginine and NG-nitro- L-arginine methyl ester. Clin Exp Pharmacol Physiol. 1998;25(11):908-912. google scholar
  41. Iseri SO, Ersoy Y, Ercan F, et al. The effect of silde-nafil, a phosphodiesterase-5 inhibitor, on acetic acid-induced colonic inflammation in the rat. J Gastroenterol Hepatol. 2009;24(6):1142-1148. google scholar
  42. Arabacı Tamer S, Akbulut S, Erdoğan Ö, et al. Neuropeptide W exhibits preventive and therapeutic effects on acetic acid-induced colitis via modulation of the cyclooxygenase enzyme system. Dig Dis Sci. 2023;68(6):2441-2453. google scholar
  43. Moga DK, Adipo N, Matu EN, Kirira PG. Antioxidant and an-tiproliferative activity of Azadirachta indica A. Juss extracts against cancer cell lines: An experimental study. Afr J Health Sci. 2012;34(5):650-656. google scholar
  44. Sarikurkcu C, Locatelli M, Mocan A, Zengin G, Kirkan B. Phe-nolic profile and bioactivities of Sideritis perfoliata L.: The plant, its most active extract, and its broad biological properties. Front Pharmacol. 2020;10:1642. doi:10.3389/fphar.2019.01642 google scholar
  45. Lu Y, Han X. Therapeutic implications of phenolic acids for ameliorating inflammatory bowel disease. Nutrients. 2024;16(9):1347. doi:10.3390/nu16091347 google scholar
  46. Vezza T, Rodriguez-Nogales A, Algieri F, et al. Flavonoids in inflammatory bowel disease: A review. Nutrients. 2016;8(4):211-232. google scholar
  47. Biasi F, Leonarduzzi G, Oteiza PI, Poli G. Inflammatory bowel disease: Mechanisms, redox considerations, and therapeutic tar-gets. Antioxid Redox Signal. 2013;19(14):1711-1747. google scholar
  48. Roda G, Marocchi M, Sartini A, Roda E. Cytokine networks in ulcerative colitis. Ulcers. 2011; 2011(1):1-5 google scholar
  49. Xue JC, Yuan S, Hou XT, et al. Natural products modulate NLRP3 in ulcerative colitis. Front Pharmacol. 2023;14:1265825. doi:10.3389/fphar.2023.1265825 google scholar
  50. Badr G, Elsawy H, Amalki MA, et al. Protective effects of myris-ticin against ulcerative colitis induced by acetic acid in male mice. Food Agr Immunol. 2020;31(1):435-446. google scholar
  51. Yamamoto T, Saniabadi AR, Umegae S, Matsumoto K. Impact of selective leukocytapheresis on mucosal inflammation and ulcer-ative colitis: Cytokine profiles and endoscopic findings. Inflamm Bowel Dis. 2006;12(8):719-726. google scholar
  52. Buran T, Batır MB, Çam FS, et al. Molecular analyses of ADAMTS-1, -4, -5, and IL-17 a cytokine relationship in pa-tients with ulcerative colitis. BMC Gastroenterol. 2023;23(1):345. doi:10.1186/s12876-023-02985-z google scholar
  53. Fujino S, Andoh A, Bamba S, et al. Increased expression of inter-leukin 17 in inflammatory bowel disease. Gut. 2003;52(1):65-70. google scholar
  54. Niu Z, Li X, Yang X, Sun Z. Protective effects of sinome-nine against dextran sulfate sodium-induced ulcerative colitis in rats via alteration of HO-1/Nrf2 and inflammatory pathway. Inflammopharmacology. 2024;32(3):2007-2022. google scholar
  55. Han Y, Ma TM, Lu ML, et al. Role of moxibustion in inflamma-tory responses during treatment of rat ulcerative colitis. World J Gastroenterol. 2014;20(32):11297-11304. google scholar
  56. Sanchez-Munoz F, Fonseca-Camarillo GC, Villeda-Ramirez MA, et al. TLR9 mRNA expression is upregulated in patients with active ulcerative colitis. Inflamm Bowel Dis. 2010;16(8):1267-1268. google scholar
  57. Sanchez-Munoz F, Fonseca-Camarillo G, Villeda-Ramirez MA, et al. Transcript levels of Toll-Like Receptors 5, 8 and 9 cor-relate with inflammatory activity in ulcerative colitis. BMC Gastroenterol. 2011;11:138. doi:10.1186/1471-230X-11-138 google scholar
  58. Atreya R, Bloom S, Scaldaferri F, et al. Clinical effects of a topically applied toll-like receptor 9 agonist in active moderate-to-severe ulcerative colitis. J Crohns Colitis. 2016;10(11):1294-1302. google scholar
  59. Atreya R, Reinisch W, Peyrin-Biroulet L, et al. Clinical efficacy of the Toll-like receptor 9 agonist cobitolimod using patient-reported-outcomes defined clinical endpoints in patients with ul-cerative colitis. Dig Liver Dis. 2018;50(10):1019-1029. google scholar
  60. Ren MT, Gu ML, Zhou XX, et al. Sirtuin 1 alleviates endoplasmic reticulum stress-mediated apoptosis of intestinal epithelial cells in ulcerative colitis. World J Gastroenterol. 2019;25(38):5800-5813. google scholar
  61. Sun J, Zhang H, Guan L, Zhou H, Sun M. Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative colitis in mice. Int J Clin Exp Med. 2015;8(1):358-367. google scholar
  62. Zhu L, Dai LM, Shen H, et al. Qing Chang Hua Shi granule ame-liorate inflammation in experimental rats and cell model of ulcera-tive colitis through MEK/ERK signaling pathway [published cor-rection appears in Biomed Pharmacother. 2023 Jul;163:114854. doi: 10.1016/j.biopha.2023.114854]. Biomed Pharmacother. 2019;116:108967. doi:10.1016/j.biopha.2019.108967 google scholar
  63. Khajah MA, Orabi KY, Hawai S, Sary HG, El-Hashim AZ. Onion bulb extract reduces colitis severity in mice via modulation of colonic inflammatory pathways and the apoptotic machinery. J Ethnopharmacol. 2019;241:31-40. google scholar
  64. Kobayashi K, Arimura Y, Goto A, et al. Therapeutic implications of the specific inhibition of causative matrix metalloproteinases in experimental colitis induced by dextran sulphate sodium. J Pathol. 2006;209(3):376-383. google scholar
  65. Marönek M, Marafini I, Gardlik R, et al. Metalloproteinases in inflammatory bowel diseases. J Inflamm Res. 2021;14:1029-1041. google scholar