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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2026 / Volume: 53 Issue: 1

Characterization of Staphylococcus aureus strains isolated from subclinic bovine mastitis by protein patterns, antibiotic resistance and plasmid profile

Subklinik inek mastitislerinden izole edilen Staphylococcus aureus suşlarının protein paterni, antibiyotik direnci ve plazmid profili yönünden karakterizasyonu

Özkan Aslantaş(Mustafa Kemal Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Hatay)
Fatma Öztürk(Gazi Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, 06500, Ankara)
Ayten Çelebi(Kırıkkale Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Kırıkkale)
Leyla Açık(Gazi Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, 06500, Ankara)
Yaşar Ergün(Mustafa Kemal Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Hatay .)
Pages
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Publication Date
2006-03-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Plasmid profiling demonstrated that 47 of the 50 S. aureus strains contained plasmid ranging from 1.7 to >24 kb.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:A total of 50 Staphylococcus aureus strains isolated from bovine subclinical mastitis in Hatay region were characterized by protein patterns, antibiotic resistance and plasmid profiles.
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Key Findings & Data:SDS-PAGE analysis of whole-cell protein extracts belong to S. aureus strains produced patterns containing 17-49 discrete bands with molecular weights of >14.4-<116 kDa.
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Conclusion & Impact:Plasmid profiling demonstrated that 47 of the 50 S. aureus strains contained plasmid ranging from 1.7 to >24 kb.
🔬Design:Letter to Editor
📍Scope:Comprehensive Sample
⚙️Variables:Mastitis · plasmid · protein

Abstract

A total of 50 Staphylococcus aureus strains isolated from bovine subclinical mastitis in Hatay region were characterized by protein patterns, antibiotic resistance and plasmid profiles. SDS-PAGE analysis of whole-cell protein extracts belong to S. aureus strains produced patterns containing 17-49 discrete bands with molecular weights of >14.4-<116 kDa. S. aureus strains clustered into two cluster on the basis of a computer-assisted numerical analysis. The resistance rates of 50 of S. aureus strains to trimethoprim-sulphamethoxazole, danofloxacin, amoxicillin-clavulanic, sulbactam-ampicillin, erythromycin, lincomycin, cefoperazone, oxytetracycline, amoxicillin, streptomycin and penicillin were 4 %, 4 %, 10 %, 14 %, 30 %, 34 %, 36 %, 38 %, 50 %, 54 % and 68 %, respectively. Plasmid profiling demonstrated that 47 of the 50 S. aureus strains contained plasmid ranging from 1.7 to >24 kb.

Özet

Hatay bölgesindeki subklinik inek mastitislerinden izole edilen toplam 50 Staphylococcus aureus suşunun protein paterni, antibiyotik dirençliliği ve plazmid profili yönünden karakterizasyonu yapıldı. S. aureus suşlarının SDS-PAGE ile analizinde moleküler ağırlığı 116 kDa arasında değişen 17-49 bant elde edildi. Elektroforetik protein bantlarının bilgisayar destekli numerikal analizi sonucunda S. aureus suşları 2 gruba ayrıldı. S. aureus suşları trimethoprim-sülfametoksazol, danofloksasin, amoksisilin-klavulanik asit, sulbaktam-ampisilin, eritromisin, linkomisin, sefoperazon, oksitetrasiklin, amoksisilin, streptomisin ve penisilin’e sırasıyla % 4, % 4, % 10, % 14, % 30, % 34, % 36, % 38, % 50, % 54 ve % 68 oranlarında dirençli bulundu. İncelenen izolatların 47’sinde, büyüklüğü 1.7->24 kb arasında değişen plazmidler saptandı

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Cite This Article

Aslantaş, Ö., Öztürk, F., Çelebi, A., Açık, L., Ergün, Y. (2006). Characterization of Staphylococcus aureus strains isolated from subclinic bovine mastitis by protein patterns, antibiotic resistance and plasmid profile. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Volume: 53 Issue: 1. https://doi.org/10.1501/Vetfak_0000000057

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