For the first time, in Turkey, the draft genome of S. felis was obtained and made publicly accessible.
📌 3 Key Takeaways
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Objective & Hypothesis:Staphylococcus felis is one of staphylococci residing on skin of cats, and has been increasingly reported as a potential facultative pathogen.
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Key Findings & Data:A recently isolated S. felis (named S. felis HARRANVET strain) from necrotizing fasciitis case of a cat was characterized using whole genome sequencing (WGS), and assembled genome was in silico screened for putative virulence and antimicrobial resistance genes.
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Conclusion & Impact:For the first time, in Turkey, the draft genome of S. felis was obtained and made publicly accessible.
Staphylococcus felis is one of staphylococci residing on skin of cats, and has been increasingly reported as a potential facultative pathogen. A recently isolated S. felis (named S. felis HARRANVET strain) from necrotizing fasciitis case of a cat was characterized using whole genome sequencing (WGS), and assembled genome was in silico screened for putative virulence and antimicrobial resistance genes. The isolate was pan-susceptible to all antimicrobials tested, indicated absence of resistance genes. A limited number of virulence genes associated with adhesion was found. For the first time, in Turkey, the draft genome of S. felis was obtained and made publicly accessible.
Qekwana DN, Sebola D, Oguttu JW, Odoi A. Antimicrobial resistance patterns of Staphylococcus species isolated from cats presented at the veterinary academic hospital in South Africa. BMC Vet Res, 2017; 13:286
Bierowiec K, Korzeniowska-Kowal A, Wzorek A, Rypuła K, Gamian A. Prevalence of Staphylococcus Species Colonization in Healthy and Sick Cats. Biomed Res Int, 2019; 2019:4360525
Kwaszewska A, Lisiecki P, Szemraj M, Szewczyk EM. Animal Staphylococcus felis with the potential to infect human skin. Med Dosw Mikrobiol, 2015; 67:69-78
Litster A, Moss SM, Honnery M, Rees B, Trott DJ. Prevalence of bacterial species in cats with clinical signs of lower urinary tract disease: Recognition of Staphylococcus felis as a possible feline urinary tract pathogen. Vet Microbiol, 2007; 121(1-2):182–188
Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC. The Gram-Positive Cocci. In, Color Atlas and Textbook of Diagnostic Microbiology. 5th ed., Lippincott Raven Publishers, Philadelphia; 1997, p. 539-565
CLSI. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement, CLSI document M100-S22, Wayne, PA: Clinical and Laboratory Standart Institute, 2012
Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol, 2012; 19:455-477
Langmead B, Salzberg S: Fast gapped-read alignment with Bowtie 2. Nature Methods, 2012; 9:357-359
Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, et al. Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother, 2012; 67:2640–2644
Joensen KG, Scheutz F, Lund O, Hasman H, Kaas RS, Nielsen EM, et al. Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia coli. J Clin Microbiol, 2014; 52:1501–1510
Brettin T, Davis JJ, Disz T, EdwardsRA, GerdesS, OlsenGJ, et al. RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci Rep, 2015; 5:8365
Worthing K, Pang S, Trott DJ, Abraham S, Coombs GW, Jordan D, et al. Characterisation of Staphylococcus felis isolated from cats using whole genome sequencing. Vet Microbiol, 2018; 222:98-104. 14.