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Journals / Anatolian Journal of Botany / 2026 / Cilt: 10 Sayı: 2

Entoloma hirtipes in Türkiye: First molecular confirmation based on ITS from Çanakkale

Çanakkale'den Entoloma hirtipes: Türkiye'deki ilk ITS temelli moleküler doğrulama

İsmail Acar(YÜZÜNCÜ YIL ÜNİVERSİTESİ, BAŞKALE MESLEK YÜKSEKOKULU)0000-0002-6049-4896
Halide Karabıyık(TRAKYA UNIVERSITY)0000-0002-1778-2200
Pages
215–222
Publication Date
2026-09-18
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study provides the first ITS rDNA-based molecular confirmation of E. hirtipes from Türkiye, expands the documented distribution of the species within the country, and demonstrates the importance of integrative taxonomy for the accurate delimitation of morphologically similar species in Entolomataceae.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Entoloma hirtipes (Schumach.) M.M.
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Key Findings & Data:Moser is a member of the Entolomataceae characterized by considerable morphological variability and taxonomic complexity, making accurate species delimitation challenging.
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Conclusion & Impact:This study provides the first ITS rDNA-based molecular confirmation of E. hirtipes from Türkiye, expands the documented distribution of the species within the country, and demonstrates the importance of integrative taxonomy for the accurate delimitation of morphologically similar species in Entolomataceae.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Entolomataceae · fungal taksonomi · ITS rDNA

Abstract

Entoloma hirtipes (Schumach.) M.M. Moser is a member of the Entolomataceae characterized by considerable morphological variability and taxonomic complexity, making accurate species delimitation challenging. In this study, a macrofungal specimen collected from Çanakkale Province in northwestern Türkiye was identified using an integrative taxonomic approach combining detailed macro- and micromorphological observations with ITS rDNA-based phylogenetic analyses. Macroscopic and microscopic examinations included the assessment of basidiospores, basidia, cheilocystidia, and pileipellis structures, which were compared with published descriptions. The examined material exhibited diagnostic features consistent with E. hirtipes, including a hygrophanous pileus, a fibrillose stipe surface, and heterodiametric angular basidiospores. The ITS region was amplified using the ITS1/ITS4 primer pair and analysed together with reference sequences retrieved from GenBank. Bayesian phylogenetic analysis recovered the newly generated sequence within a strongly supported E. hirtipes clade (PP = 1.00), fully corroborating the morphological identification. The combined morphological and molecular evidence enabled the reliable identification of the examined material as E. hirtipes. This study provides the first ITS rDNA-based molecular confirmation of E. hirtipes from Türkiye, expands the documented distribution of the species within the country, and demonstrates the importance of integrative taxonomy for the accurate delimitation of morphologically similar species in Entolomataceae.

Özet

Entoloma hirtipes (Schumach.) M.M. Moser, belirgin morfolojik değişkenlik ve taksonomik karmaşıklık gösteren Entolomataceae üyelerinden biri olup, doğru tür teşhisini güçleştirmektedir. Bu çalışmada, Türkiye'nin kuzeybatısında yer alan Çanakkale ilinden toplanan bir makromantar örneği, ayrıntılı makro- ve mikromorfolojik incelemeler ile ITS rDNA temelli filogenetik analizlerin birlikte kullanıldığı integratif taksonomik bir yaklaşımla değerlendirilmiştir. Makroskobik ve mikroskobik incelemeler kapsamında basidiyospor, bazidyum, keilosistidya ve pileipellis yapıları ayrıntılı olarak incelenmiş ve literatürde bildirilen verilerle karşılaştırılmıştır. İncelenen örneğin higrofan şapka yapısı, fibrilloz sap yüzeyi ve heterodiametrik, köşeli basidiyosporları gibi ayırt edici karakterlerinin E. hirtipes tür tanımıyla uyumlu olduğu belirlenmiştir. ITS bölgesi ITS1/ITS4 primer çifti kullanılarak çoğaltılmış ve GenBank'tan elde edilen referans dizilerle birlikte analiz edilmiştir. ITS rDNA veri setine dayalı Bayesyen filogenetik analiz sonucunda elde edilen yeni dizi, güçlü destek değerine sahip (PP = 1.00) E. hirtipes kladı içerisinde konumlanmış ve morfolojik teşhisi moleküler olarak doğrulamıştır. Morfolojik ve moleküler verilerin birlikte değerlendirilmesi, incelenen materyalin güvenilir biçimde E. hirtipes olarak tanımlanmasını sağlamıştır. Bu çalışma, E. hirtipes'in Türkiye'den ITS rDNA verilerine dayalı ilk moleküler doğrulamasını sunmakta, türün Türkiye'deki bilinen yayılışını genişletmekte ve Entolomataceae içerisinde morfolojik olarak benzer türlerin doğru şekilde sınırlandırılmasında integratif taksonominin önemini ortaya koymaktadır.

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Cite This Article

Acar, İ., Karabıyık, H. (2026). Entoloma hirtipes in Türkiye: First molecular confirmation based on ITS from Çanakkale. Anatolian Journal of Botany, Cilt: 10 Sayı: 2, 215–222. https://doi.org/10.30616/ajb.1968581

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