Journals / Anadolu Tarım Bilimleri Dergisi / 2017 / Cilt: 32 - Sayı: 1

Effects of some organic and inorganic compounds on antioxidative system in pepper (Capsicum annuum L.) plant under salt stress

Tuz stresi altindaki biber (Capsicum Annuum L.) bitkisinde bazi organik ve inorganik bileşiklerin antioksidatif sisteme etkileri

Pages
121–131
Publication Date
2017-02-13
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Consequently, it was thought that the ones having the greatest effect on diminishing salinity stress and the ability of regulating plant metabolism by supporting antioxidative system among organic and inorganic compounds, of which activities were compared in pepper plant grown under salinity stress and controlled conditions, could be used under salinity.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study conducted as a pot experiment under laboratory conditions, in addition to NaCl (100 mM), some organic compounds, ascorbic acid (0.5 mM), salicylic acid (0.1 mM), nitric oxide (as SNP;100 µM), proline (10 mM) and some inorganic compounds such as silicon (2 mM), calcium (10 mM) and potassium (10 mM) were applied to pepper plant foliarly.
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Key Findings & Data:It was observed that membrane permeability, lipid peroxidation, proline content and antioxidative enzyme activities increased in pepper leaves under ascending salinity stress, however, these parameters decreased with the addition of some organic and inorganic compounds.
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Conclusion & Impact:Consequently, it was thought that the ones having the greatest effect on diminishing salinity stress and the ability of regulating plant metabolism by supporting antioxidative system among organic and inorganic compounds, of which activities were compared in pepper plant grown under salinity stress and controlled conditions, could be used under salinity.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Salinity · Pepper · Exogenous applications

Abstract

In this study conducted as a pot experiment under laboratory conditions, in addition to NaCl (100 mM), some organic compounds, ascorbic acid (0.5 mM), salicylic acid (0.1 mM), nitric oxide (as SNP;100 µM), proline (10 mM) and some inorganic compounds such as silicon (2 mM), calcium (10 mM) and potassium (10 mM) were applied to pepper plant foliarly. Thus, general development situation in pepper plants under salt stress as well as comparative effects of these organic and inorganic compounds on lipid peroxidation and antioxidative defense system of pepper plant were investigated. At the end of analyses made during the study, membrane permeability (EC %), proline, chlorophyll and carotenoid contents, some antioxidative enzyme activities (SOD, POX CAT) were revealed, furthermore some plant growth parameters were determined. At the same time, the content of some macro elements (Na, Ca, K and P) in leaves were analyzed. It was observed that membrane permeability, lipid peroxidation, proline content and antioxidative enzyme activities increased in pepper leaves under ascending salinity stress, however, these parameters decreased with the addition of some organic and inorganic compounds. Additionally, the content of leaf macro element, which showed a decline with salinity, and also plant length, stem diameter, stem-root fresh and dry weights changed positively with the addition of some organic and inorganic compounds to the growth media. It was observed that among these compounds AsA, NO and K were the most effective ones for suppression of lipid peroxidation, the regulation of enzymatic antioxidative system and leaf dry weight, respectively. Consequently, it was thought that the ones having the greatest effect on diminishing salinity stress and the ability of regulating plant metabolism by supporting antioxidative system among organic and inorganic compounds, of which activities were compared in pepper plant grown under salinity stress and controlled conditions, could be used under salinity.

Özet

Laboratuvar şartlarında saksı denemesi şeklinde yürütülmüş olan bu çalışmada biber (Capsicum annuum L.) bitkisine NaCl (100 mM) yanı sıra, organik bileşiklerden: askorbik asit (0.5 mM), salisilik asit (0.1 mM), nitrik oksit (Sodium nitroprusside; 100 µM), prolin (10 mM) ve inorganik bileşiklerden: silisyum (2 mM), kalsiyum (10 mM) ve potasyum (10 mM) yaprak yoluyla uygulanmıştır. Tuz stresi altındaki biber bitkisinde genel vejetatif gelişme durumu ile bu organik ve inorganik bileşiklerin biber bitkisinin lipit peroksidasyon düzeyleri ve antioksidatif savunma sistemi üzerine etkinlikleri araştırılmıştır. Deneme sonunda yapılan analizlerde; yapraklarda membran geçirgenliği (% EC), prolin, klorofil ve karotenoid kapsamları, antioksidatif enzimlerden SOD, POX, CAT aktiviteleri tayini yapılmış, ayrıca bazı bitki gelişim parametreleri saptanmıştır. Aynı zamanda, yapraklarda bazı makro elementlerin (Na, Ca, K ve P ) içeriği belirlenmiştir. Araştırma sonunda elde edilen verilere göre; artan tuz stresi altında biber bitkisi yapraklarında elektriksel geçirgenlik, lipid peroksidasyonu, prolin içeriği ve antioksidatif enzim aktivitelerinde artış, bazı organik ve inorganik bileşiklerin tuzlu ortama ilavesiyle bu parametrelerde düşüş gözlenmiştir. Bunun yanı sıra tuzlulukla beraber azalma göstermiş olan yaprak makro element içeriği, gövde ve kök yaş ve kuru ağırlığı ortama bazı organik ve inorganik bileşiklerin eklenmesiyle olumlu olarak değişim göstermiştir. Bu bileşikler arasında lipit peroksidasyonun regülasyonunda en iyi etkiyi AsA, enzimatik antioksidatif sistemin regülasyonunda NO ve yaprak kuru ağırlığında ise K göstermiştir. Kontrollü şartlarda ve tuz stresi altında yetiştirilen biber bitkisinde etkinlikleri karşılaştırılmış olan organik ve inorganik bileşiklerden antioksidatif sistemi destekleyerek, bitki metabolizmasını düzenleyici ve tuz stresini hafifletmede en fazla etkiye sahip olan bileşiklerin tuzlu şartlarda kullanılabileceği ön yargısına varılmıştır.

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Cite This Article

Tuna, A. L., Eroğlu, B. (2017). Effects of some organic and inorganic compounds on antioxidative system in pepper (Capsicum annuum L.) plant under salt stress. Anadolu Tarım Bilimleri Dergisi, Cilt: 32 - Sayı: 1, 121–131. https://ajindex.com/articles/135473

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