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Journals / Adıyaman Üniversitesi Fen Bilimleri Dergisi / 2026 / Cilt: 16 Sayı: 1

Multiplex PCR-based Molecular Characterization of Fecal-Derived Multidrug-Resistant Escherichia coli Isolates

Gaita Kaynaklı Çoklu İlaç Dirençli Escherichia coli İzolatlarının Multipleks PZR ile Moleküler Karakterizasyonu

Doğan Sarigül(Hatay Mustafa Kemal University)0009-0000-7400-8695
Berfin Berin Kizildeli(ADIYAMAN ÜNİVERSİTESİ)0009-0005-0378-6939
Birgül Özcan(Hatay Mustafa Kemal University)0000-0001-9003-8480
Muhsin Aydın(ADIYAMAN ÜNİVERSİTESİ)0000-0002-1204-1163
Pages
46–60
Publication Date
2026-07-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results showed that 82.25% of isolates carried none of the targeted bla genes.

📌 3 Key Takeaways

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Objective & Hypothesis:Antimicrobial resistance (AMR) in Escherichia coli presents a serious public health threat, particularly when resistant strains spread through contaminated food and water.
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Key Findings & Data:This study characterized fecal-derived multidrug-resistant (MDR) E. coli isolates using multiplex polymerase chain reaction (PCR) to detect extended-spectrum β-lactamase (ESBL) gene variants (blaTEM, blaOXA, blaSHV, and blaCTX-M), sulfonamide resistance genes (sul1, sul2, and sul3), and integron-associated integrase genes (int1, int2, and int3).
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Conclusion & Impact:Results showed that 82.25% of isolates carried none of the targeted bla genes.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Escherichia coli · multipleks PZR · GSBL

Abstract

Antimicrobial resistance (AMR) in Escherichia coli presents a serious public health threat, particularly when resistant strains spread through contaminated food and water. This study characterized fecal-derived multidrug-resistant (MDR) E. coli isolates using multiplex polymerase chain reaction (PCR) to detect extended-spectrum β-lactamase (ESBL) gene variants (blaTEM, blaOXA, blaSHV, and blaCTX-M), sulfonamide resistance genes (sul1, sul2, and sul3), and integron-associated integrase genes (int1, int2, and int3). We analyzed 62 uspA-positive E. coli isolates. Results showed that 82.25% of isolates carried none of the targeted bla genes. The blaCTX-M and blaOXA genes were present in 8.06% and 9.67% of isolates, respectively. Co-occurrence of bla and sul genes appeared in 12.91% of isolates. Co-occurrence of bla and int genes occurred in 12.90%. Only 6.45% of isolates carried at least one gene from all three categories, indicating potential MDR genotypes. These findings highlight the importance of molecular surveillance in tracking resistance genes outside clinical settings. They support the need for integrated One Health strategies to combat AMR.

Özet

Escherichia coli'de antimikrobiyal direnç (AMR), özellikle dirençli suşlar kontamine gıda ve su yoluyla yayıldığında ciddi bir halk sağlığı tehdidi oluşturmaktadır. Bu çalışmada, gaita kaynaklı çoklu ilaç dirençli (MDR) E. coli izolatları, genişlemiş spektrumlu beta-laktamaz (GSBL) gen varyantlarını (blaTEM, blaOXA, blaSHV ve blaCTX-M), sülfonamid direnç genlerini (sul1, sul2 ve sul3) ve integron ilişkili integraz genlerini (int1, int2 ve int3) tespit etmek amacıyla multipleks polimeraz zincir reaksiyonu (PZR) kullanılarak karakterize edilmiştir. uspA-pozitif 62 E. coli izolatı analiz edilmiştir. Sonuçlar, izolatların %82,25'inin hedeflenen bla genlerinin hiçbirini taşımadığını göstermiştir. blaCTX-M ve blaOXA genleri sırasıyla izolatların %8,06 ve %9,67'sinde tespit edilmiştir. bla ve sul genlerinin birlikte bulunma oranı izolatların %12,91'inde görülmüştür. bla ve int genlerinin birlikte bulunma oranı ise %12,90 olarak gerçekleşmiştir. İzolatların yalnızca %6,45'i her üç kategoriden en az bir gen taşımış olup bu durum potansiyel MDR genotiplerine işaret etmektedir. Bu bulgular, direnç genlerinin klinik ortamlar dışında izlenmesinde moleküler sürveyansın önemini vurgulamaktadır. Ayrıca, AMR ile mücadelede entegre Tek Sağlık stratejilerine duyulan ihtiyacı desteklemektedir.

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Cite This Article

Sarigül, D., Kizildeli, B. B., Özcan, B., Aydın, M. (2026). Multiplex PCR-based Molecular Characterization of Fecal-Derived Multidrug-Resistant Escherichia coli Isolates. Adıyaman Üniversitesi Fen Bilimleri Dergisi, Cilt: 16 Sayı: 1, 46–60. https://doi.org/10.37094/adyujsci.1919076

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