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Journals / Harran Tarım ve Gıda Bilimleri Dergisi / 2026 / Cilt: 28 Sayı: 3

Effects of olive leaf extract on lipid oxidation in butter, sunflower oil and potato chips

Zeytin yaprağı özütünün tereyağı, ayçiçek yağı ve patates cipsinde lipit oksidasyonu üzerindeki etkileri

Aziz Korkmaz(MARDİN ARTUKLU ÜNİVERSİTESİ)0000-0002-5221-6722
Sevim Gaser0009-0003-2791-7839
Ahmet Ferit Atasoy(HARRAN ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ)0000-0002-3390-1177
Pages
489–499
Publication Date
2024-09-28
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results of the study showed that OLE, as a natural antioxidant, could be an alternative to BHT in preventing lipid oxidation.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Lipid oxidation causes sensory quality defects in edible oils and also poses a health risk.
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Key Findings & Data:For this purpose, both antioxidants were added to butter and sunflower oil at different concentrations (50, 100, 200 ppm).
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Conclusion & Impact:The results of the study showed that OLE, as a natural antioxidant, could be an alternative to BHT in preventing lipid oxidation.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Zeytin yaprağı özütü · oksidasyon · antioksidan

Abstract

Lipid oxidation causes sensory quality defects in edible oils and also poses a health risk. In this study, the antioxidant properties of an extract obtained from olive leaves (OLE) and butylated hydroxytoluene (BHT), a synthetic antioxidant, were compared in butter, sunflower oil, and potato chips. For this purpose, both antioxidants were added to butter and sunflower oil at different concentrations (50, 100, 200 ppm). Sunflower oil was used in the production of potato chips at 170±5 °C, while butter was not subjected to any heat treatment. After the accelerated shelf life test, the oxidation parameters of the samples were determined by measuring thiobarbituric acid (TBA) and peroxide values (PV). Additionally, the total antioxidant capacities (TAC) of OLE and BHT were compared based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. The oxidation parameters of OLE added butter, heat-treated sunflower oil, and potato chips were found to be significantly lower than those of the samples without any antioxidant component added (control) (P<0.05). In butters with BHT added at 50, 100, and 200 ppm concentrations, the formation of TBA was reduced by 48,92%, 47,76%, and 50,97%, respectively, compared to the samples with OLE added at the same concentrations. Similar rates were also determined for PV of butter. In sunflower oil samples, the TBA values of samples with OLE added were found to be lower (P<0.05). However, the use of BHT and OLE at concentrations of 100 and 200 ppm did not result in a significant difference in the PV of this oil (P>0.05). The TBA contents of potato chips with OLE added were found to be significantly lower than those of samples with BHT (P<0.05). In terms of TAC, OLE (79,12 mg TE (trolox equivalent) g-1) exhibited a higher value than BHT (67,39 mg TE g-1) (P < 0.05). The results of the study showed that OLE, as a natural antioxidant, could be an alternative to BHT in preventing lipid oxidation.

Özet

Lipit oksidasyonu, yemeklik yağlarda duyusal kalite kusurlarına neden olmakla birlikte tüketici sağlığı açısından da risk oluşturan bir bozulma tipidir. Bu çalışmada, zeytin yaprağından elde edilen bir özüt (ZYÖ) ile sentetik bir antioksidan olan bütillenmiş hidroksitoluenin (BHT) tereyağı, ayçiçek yağı ve patates cipsindeki antioksidan özellikleri karşılaştırılmıştır. Bu amaçla, her iki antioksidan farklı konsantrasyonlarda (50, 100, 200 ppm) tereyağına ve ayçiçek yağına ilave edilmiş, bunlardan ayçiçek yağı kızartma yöntemiyle (170±5°C) patetes cipsi üretiminde kullanılırken tereyağı ise herhangi bir ısıl işleme tabi tutulmamıştır. Hızlandırılmış raf ömrü testinden sonra oksidasyon parametreleri olarak örneklerin tiyobarbitürik asit (TBA) içerikleri ve peroksit sayıları (PS) belirlenmiştir. Ayrıca, 2,2-difenil-1-pikrilhidrazil (DPPH) radikali giderme aktivitesine dayalı olarak ZYÖ ile BHT’nin toplam antioksidan kapasitleri (TAK) de karşılaştırılmıştır. ZYÖ ilave edilmiş tereyağı, ısıl işlem görmüş ayçiçek yağı ve patates cipsinin oksidasyon göstergeleri herhangi bir antioksidan bileşen eklenmemiş örneklerinkine (kontrol) nazaran önemli oranda düşük bulunmuştur (P<0.05). 50, 100 ve 200 ppm kosantrasyonlarında BHT eklenmiş tereyağlarında, aynı konsantrasyonlarda ZYÖ ilave edilmiş örneklere göre sırasıyla %48,92, %47,76 ve %50,97 oranında daha düşük miktarlarda TBA oluşmuştur. Benzer oranlar, tereğının PS değerleri için de saptanmıştır. Ayçiçek yağı örneklerinde ise ZYÖ eklenmiş örneklerin TBA değerleri BHT eklenmiş örneklerin ve kontrol örneğinin TBA değerlerine göre daha düşük bulunmuştur (P<0.05). Ancak, 100 ve 200 ppm’lik konsantrasyonlarda BHT ve ZYÖ kullanımı bu yağın PS düzeyi için anlamlı bir fark oluşturmamıştır (P>0.05). ZYÖ eklenmiş patates cipslerinin TBA içerikleri BHT’li örneklerinkine göre önemli düzeyde daha düşük bulunmuştur (P<0.05). TAK bakımından ise ZYÖ (79,12 mg TE (troloks eşdeğeri) g-1) BHT’ye (67,39 mg TE g-1) nazaran daha yüksek değer sergilemiştir (P<0.05). Çalışmanın sonuçları, doğal bir antioksidan olarak ZYÖ’nun lipit oksidasyonlarının önlenmesinde BHT’ye alternatif olabileceğini göstermiştir.

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Cite This Article

Korkmaz, A., Gaser, S., Atasoy, A. F. (2024). Effects of olive leaf extract on lipid oxidation in butter, sunflower oil and potato chips. Harran Tarım ve Gıda Bilimleri Dergisi, Cilt: 28 Sayı: 3, 489–499. https://doi.org/10.29050/harranziraat.1460295

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References (23)

  1. Baccouri, B., Rajhi, I., Theresa, S., Najjar, Y., Mohamed, S. N., & Willenberg, I. (2022). The potential of wild olive leaves (Olea europaea L. subsp. oleaster) addition as a functional additive in olive oil production: the effects on bioactive and nutraceutical compounds using LC–ESI–QTOF/MS. European Food Research and Technology, 248(11), 2809–2823. https://doi.org/10.1007/s00217-022-04091-y
  2. Ben Salah, M., & Abdelmelek, H. (2012). Study of Phenolic Composition and Biological Activities Assessment of Olive Leaves from different Varieties Grown in Tunisia. Medicinal Chemistry, 2(5), 107-11 https://doi.org/10.4172/2161-0444.1000124
  3. Chammem, N., Saoudi, S., Sifaoui, I., Sifi, S., de Person, M., Abderraba, M., Moussa, F., & Hamdi, M. (2015). Improvement of vegetable oils quality in frying conditions by adding rosemary extract. Industrial Crops and Products, 74, 592–599. https://doi.org/10.1016/j.indcrop.2015.05.054
  4. Çelik, Ş., Doğru, E., Yakar, Y., & Ünver, N. (2021). Zeytin yaprağı ilavesinin zeytinyağının bazı karakteristik özelliklerine etkisi. Harran Tarım ve Gıda Bilimleri Dergisi, 25(1), 72–85. https://doi.org/10.29050/harranziraat.744568
  5. Demirkaya, A. (2013) Tereyağında Tiyobarbiturik Asit (TBA) Testi ile Lipid Oksidasyonunun Değerlendirilmesi. Atatürk University Journal of Veterinary Sciences, 8(3), 237–240.
  6. Difonzo, G., Squeo, G., Pasqualone, A., Summo, C., Paradiso, V. M., & Caponio, F. (2021). The challenge of exploiting polyphenols from olive leaves: addition to foods to improve their shelf‐life and nutritional value. Journal of the Science of Food and Agriculture, 101(8), 3099–3116. https://doi.org/10.1002/jsfa.10986
  7. Esposto, S., Taticchi, A., Di Maio, I., Urbani, S., Veneziani, G., Selvaggini, R., Sordini, B., & Servili, M. (2015). Effect of an olive phenolic extract on the quality of vegetable oils during frying. Food Chemistry, 176, 184–192. https://doi.org/10.1016/j.foodchem.2014.12.036
  8. Farag, R. S., Mahmoud, E. A., & Basuny, A. M. (2007). Use crude olive leaf juice as a natural antioxidant for the stability of sunflower oil during heating. International Journal of Food Science & Technology, 42(1), 107–115. https://doi.org/10.1111/j.1365-2621.2006.01374.x
  9. Gramza-Michalowska, A., Korczak, J., Regula, J. (2007). Use of Plant Extracts in Summer and Winter Season Butter Oxidative Stability Improvement. Asia Pacific Journal of Clinical Nutrition, 16(1), 85–88.
  10. Guo, Q., Gao, S., Sun, Y., Gao, Y., Wang, X., & Zhang, Z. (2016). Antioxidant efficacy of rosemary ethanol extract in palm oil during frying and accelerated storage. Industrial Crops and Products, 94, 82–88. https://doi.org/10.1016/j.indcrop.2016.08.032
  11. International olive council (IOC). Determination of Peroxide Value "COI/T.20/Doc. No 35/Rev.1 (2017), Erişim adresi: https://www.internationaloliveoil.org/wp-content/uploads/2019/11/Method-COI-T.20-Doc.-No-35-Rev.-1-2017.pdf.
  12. Jiménez, P., García, P., Bustamante, A., Barriga, A., & Robert, P. (2017). Thermal stability of oils added with avocado (Persea americana cv. Hass) or olive (Olea europaea cv. Arbequina) leaf extracts during the French potatoes frying. Food Chemistry, 221, 123–129. https://doi.org/10.1016/j.foodchem.2016.10.051
  13. Kamacı, S. (2021). Tereyağı eritme sıcaklığının şanlıurfa sadeyağının (urfa yağı) kalite özelliklerine olan etkisinin araştırılması (Yayımlanmamış yüksek lisans tezi). Harran Üniversitesi Fen Bilimleri Enstitüsü, Şanlıurfa.
  14. Kontogianni, V. G., & Gerothanassis, I. P. (2012). Phenolic compounds and antioxidant activity of olive leaf extracts. Natural Product Research, 26(2), 186–189. https://doi.org/10.1080/14786419.2011.582842
  15. Korkmaz, A. (2023). Characterization and Comparison of Extra Virgin Olive Oils of Turkish Olive Cultivars. Molecules, 28(3), 1483. https://doi.org/10.3390/molecules28031483
  16. Macit, A., & Kizil, M. (2022). Effect of olive leaf extract marination on heterocyclic aromatic amine formation in pan‐fried salmon. Journal of the Science of Food and Agriculture, 102(9), 3908–3915. https://doi.org/10.1002/jsfa.11740
  17. Mansour, A. W., & Sindi, H. A. (2024). Effects of Ajwa date seeds on the oxidative stability of butter. Heliyon, 10(2), e24717. https://doi.org/10.1016/j.heliyon.2024.e24717
  18. Maqbool, Z., Tahir, H. S., Nadeem, M. T., Ahmad, R. S., Yasmin, A., Khalid, W., Asar, T. O., & Al-Sameen, M. A. (2023). Stability of Butter Using Spray Dried Sweet Lime Peel (SLP) Powder. International Journal of Food Properties, 26(1), 2627–2641. https://doi.org/10.1080/10942912.2023.2241659
  19. Nikou, T., Sakavitsi, M. E., Kalampokis, E., & Halabalaki, M. (2022). Metabolism and Bioavailability of Olive Bioactive Constituents Based on In Vitro, In Vivo and Human Studies. Nutrients, 14(18), 3773. https://doi.org/10.3390/nu14183773
  20. Orozco-Solano, M. I., Priego-Capote, F., & Luque de Castro, M. D. (2011). Influence of Simulated Deep Frying on the Antioxidant Fraction of Vegetable Oils after Enrichment with Extracts from Olive Oil Pomace. Journal of Agricultural and Food Chemistry, 59(18), 9806–9814. https://doi.org/10.1021/jf2019159
  21. Romojaro, A., Sanchez-Bel, P., Serrano, M., & Pretel, M. T. (2013). Wild Edible Plants as Potential Antioxidants in Vegetables Oils. Journal of Chemistry, 2013(1), 1–4. https://doi.org/10.1155/2013/457902
  22. Wu, G., Chang, C., Hong, C., Zhang, H., Huang, J., Jin, Q., & Wang, X. (2019). Phenolic compounds as stabilizers of oils and antioxidative mechanisms under frying conditions: A comprehensive review. Trends in Food Science & Technology, 92, 33–45. https://doi.org/10.1016/j.tifs.2019.07.043
  23. Zhang, H., Tang, Z., Rasco, B., Tang, J., & Sablani, S. S. (2016). Shelf-life modeling of microwave-assisted thermal sterilized mashed potato in polymeric pouches of different gas barrier properties. Journal of Food Engineering, 183, 65–73. https://doi.org/10.1016/j.jfoodeng.2016.03.021