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Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 7 Sayı: 1

Identifcation and Characterization of Pectolytic Bacteria which is Causing Soft Rot in some Fruits and Vegetables

Bazı Sebze ve Meyvelerde Yumuşak Çürüklük Oluşturan Pektolitik Bakterilerin Tanı ve Karakterizasyonu

Fatih Dadaşoğlu
Recep Kotan
Pages
155–161
Publication Date
2017-03-31
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

P. carotovorum subsp. atrosepticum strains of identifed as pathogen in strawberry, mulberry, parsley, cabbage, potatoes, peppers and eggplant and the strains of E. chrysanthemi ; 2 of them in onion, 2 of them in pepper and one of them also in parsley, it has been found to cause soft rot disease.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, it is aimed to be determined make a defnitive diagnosis of Pectobacterium caratovorum subsp. atrosepticum and Erwinia chrysanthemi , which are the agent of Soft Rot for some fruits and vegetables.
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Key Findings & Data:For this purpose, initially microbial diagnostic systems (MIS) are defned using, causing soft rot strains in different fruits and vegetables, and 7 different strains of P. carotovorum subsp. atrosepticum and 5 different strains of E. chrysanthemi were obtained.
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Conclusion & Impact:P. carotovorum subsp. atrosepticum strains of identifed as pathogen in strawberry, mulberry, parsley, cabbage, potatoes, peppers and eggplant and the strains of E. chrysanthemi ; 2 of them in onion, 2 of them in pepper and one of them also in parsley, it has been found to cause soft rot disease.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:MIS · Moleküler tanı · Pectobacterium

Abstract

In this study, it is aimed to be determined make a defnitive diagnosis of Pectobacterium caratovorum subsp. atrosepticum and Erwinia chrysanthemi , which are the agent of Soft Rot for some fruits and vegetables. For this purpose, initially microbial diagnostic systems (MIS) are defned using, causing soft rot strains in different fruits and vegetables, and 7 different strains of P. carotovorum subsp. atrosepticum and 5 different strains of E. chrysanthemi were obtained. Also identifed using the system of BIOLOG and it was observed that the results are different from results obtained in the MIS system. According to hypersensitive reaction (HR), pathogenicity and pectolytic activity tests results it was determined that all of the pathogenic strains. Several biochemical and molecular tests (16S rDNA PCR and spesifc PCR) for the diagnosis and characterization of certain strains were made identifed as pathogen. The diagnosis of bacterial species conducted using the 16S rDNA PCR and all of the strains given band of approximately 1500 bp in length was determined. P. carotovorum subsp. atrosepticum strains of identifed as pathogen in strawberry, mulberry, parsley, cabbage, potatoes, peppers and eggplant and the strains of E. chrysanthemi ; 2 of them in onion, 2 of them in pepper and one of them also in parsley, it has been found to cause soft rot disease.

Özet

Bu çalışmada meyve ve sebzelerde yumuşak çürüklük hastalığına sebep olan Pectobacterium caratovorum subsp. atrosepticum ve Erwinia chrysanthemi türlerinin klasik ve moleküler yöntemler kullanılarak kesin tanısının yapılması hedeflenmiştir. Bu amaçla farklı meyve ve sebzelerde yumuşak çürüklük etmeni olduğu tespit edilen izolatlar ilk olarak mikrobiyal tanı sistemi (MIS) kullanılarak tanılanmış ve 7 farklı P. c. subsp. atrosepticum izolatı ile 5 farklı E. chrysanthemi izolatı elde edilmiştir. Ayrıca, strainler yine BIOLOG sistemi kullanılarak da tanılanmış olup elde edilen sonuçların MIS sisteminde alınan sonuçlardan farklı olduğu gözlemlenmiştir. Yapılan hypersensitive reaction (HR), patojenite ve pektolitik aktivite testleri sonucu bu strainlerin tamamının patojen olduğu belirlenmiştir. Patojen olduğu belirlenen strainlerin kesin tanı ve karakterizasyonu için çeşitli biyokimyasal ve moleküler testler (16S rDNA PCR ve spesifk PCR) yapılmıştır. 16S rDNA PCR testleri sonucunda strainlerin cins düzeyinde tanısı yapılmış olup, izolatların tamamının yaklaşık 1500 bp uzunluğunda bant verdiği tespit edilmiştir. Patojen olduğu belirlenen ve tanılanan P. carotovorum subsp. atrosepticum izolatlarının çilek, dut, maydanoz, lahana, patates, biber ve patlıcanda, E. chrysanthemi izolatlarının ise; 2 tanesinin soğanda, 2 tanesinin biberde ve 1 tanesinin de maydanozda yumuşak çürüklük hastalığına neden olduğu belirlenmiştir.

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Cite This Article

Dadaşoğlu, F., Kotan, R. (2017). Identifcation and Characterization of Pectolytic Bacteria which is Causing Soft Rot in some Fruits and Vegetables. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 7 Sayı: 1, 155–161. https://ajindex.com/articles/1273044

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