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Journals / Hacettepe Journal of Biology and Chemistry / 2026 / Volume: 47 Issue: 2

Decolorization of Reactıve Blue 19 Dye By Bacillus Megaterium Isolated From Soil

Özgecan Erdem(HACETTEPE UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY)
Kübra Erkan Türkmen
Doruk Aracagök
Demet Erdönmez(AKSARAY UNIVERSITY, FACULTY OF SCIENCE AND LETTERS, DEPARTMENT OF BIOLOGY, BIOLOGY PR.)
Nilüfer Cihangir
Pages
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Publication Date
2019-09-18
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In trials determining pH effect on dye removal; it was found that B. megaterium enables decolorization of Reactive Blue 19 at neutral pHs with a high percentage of 91%.

📌 3 Key Takeaways

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Objective & Hypothesis:The principle aim of this study was to decolorize Reactive Blue 19 dye with Bacillus megaterium(B. megaterium) and to determine the effect of some parameters such as pH, temperature, initial dye concentration, nitrogen and carbon sources on decolorization.
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Key Findings & Data:With this purpose, a new isolate identified as B. megaterium by genotypic (16S rRNA sequence) characterization, was found effective on Reactive Blue 19 decolorization.
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Conclusion & Impact:In trials determining pH effect on dye removal; it was found that B. megaterium enables decolorization of Reactive Blue 19 at neutral pHs with a high percentage of 91%.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Reactive Blue 19 · Bacillus megaterium · decolorization

Abstract

The principle aim of this study was to decolorize Reactive Blue 19 dye with Bacillus megaterium(B. megaterium) and to determine the effect of some parameters such as pH, temperature, initial dye concentration, nitrogen and carbon sources on decolorization. With this purpose, a new isolate identified as B. megaterium by genotypic (16S rRNA sequence) characterization, was found effective on Reactive Blue 19 decolorization. Glucose (20g/L) was found as the most suitable carbon source for Reactive Blue 19 decolorization with a yield of 92%. When the effect of nitrogen sources on decolorization was investigated, the highest dye removal rate was found as 91% which was obtained in shake flask containing yeast extract (10 g/L). In trials determining pH effect on dye removal; it was found that B. megaterium enables decolorization of Reactive Blue 19 at neutral pHs with a high percentage of 91%. P values were calculated for all parameters and found as p<0.05. Results of FTIR analysis showed that decolorization of Reactive Blue 19 by B. megaterium occurred via biodegradation. When chemical and physical wastewater treatment methods were compared to the biological methods, usage of microbial sources such as B. megaterium is considered as an efficient and economical alternative.

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Cite This Article

Erdem, Ö., Türkmen, K. E., Aracagök, D., Erdönmez, D., Cihangir, N. (2019). Decolorization of Reactıve Blue 19 Dye By Bacillus Megaterium Isolated From Soil. Hacettepe Journal of Biology and Chemistry, Volume: 47 Issue: 2. https://doi.org/10.15671/hjbc.623786

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