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Journals / Turkish Journal of Veterinary and Animal Sciences / 2006 / Cilt: 30 - Sayı: 2

Comparison of Polymerase Chain Reaction and Conventional Methods for the Diagnosis of Listeria monocytogenes in Stuffed Mussels

Ergün Ö. GÖKSOY
Şükrü KIRKAN
Osman KAYA
Pages
229–234
Publication Date
2006-02-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to evaluate the role of stuffed mussel as a source of Listeria monocytogenes.
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Key Findings & Data:A total of 50 mussel samples were investigated for L. monocytogenes.
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Conclusion & Impact:The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.
🔬Design:Research Article
📍Scope:50 samples
⚙️Variables:Listeria monocytogenes · stuffed mussel · conventional methods

Abstract

The aim of this study was to evaluate the role of stuffed mussel as a source of Listeria monocytogenes. Polymerase chain reaction is a rapid procedure with both sensitivity and specificity for quick detection and identification of L. monocytogenes. A total of 50 mussel samples were investigated for L. monocytogenes. L. monocytogenes was not identified by the conventional method. However, PCR amplification products demonstrated that 5 out of 50 samples showed positive reactions with L. monocytogenes. The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.

Özet

The aim of this study was to evaluate the role of stuffed mussel as a source of Listeria monocytogenes. Polymerase chain reaction is a rapid procedure with both sensitivity and specificity for quick detection and identification of L. monocytogenes. A total of 50 mussel samples were investigated for L. monocytogenes. L. monocytogenes was not identified by the conventional method. However, PCR amplification products demonstrated that 5 out of 50 samples showed positive reactions with L. monocytogenes. The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.

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Cite This Article

GÖKSOY, E. Ö., KIRKAN, Ş., KAYA, O. (2006). Comparison of Polymerase Chain Reaction and Conventional Methods for the Diagnosis of Listeria monocytogenes in Stuffed Mussels. Turkish Journal of Veterinary and Animal Sciences, Cilt: 30 - Sayı: 2, 229–234. https://ajindex.com/articles/122307

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