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Journals / İstanbul Üniversitesi Veteriner Fakültesi Dergisi / 2006 / Cilt: 32 - Sayı: 3

Türkiye' nin batısındaki Gökkuşağı alabalık yetiştirme çiftliklerinde Yersinia ruckeri' nin PZR kullanılarak tanımlanması

Detection of yersinia ruckeri by PCR in rainbow trout (Oncorhynchus mykıss-Walbaum) hatchery farms in the West of Turkey

Şükrü KIRKAN
Ergün Ö. GÖKSOY
Serten TEKBIYIK
Osman KAYA
Pages
21–30
Publication Date
2022-10-18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

All PCR positive samples have shown specific amplification bands of the 575-bp fragment for Yersinia ruckeri.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The presence of Yersinia ruckeri in rainbow trout hatchery farms was investigated by PCR Assay.
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Key Findings & Data:Yersinia ruckeri strains were identified from 8 out of 25 samples (32%).
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Conclusion & Impact:All PCR positive samples have shown specific amplification bands of the 575-bp fragment for Yersinia ruckeri.
🔬Design:Lab & Field Experiment
📍Scope:25 samples
⚙️Variables:Domain Parameters

Abstract

The presence of Yersinia ruckeri in rainbow trout hatchery farms was investigated by PCR Assay. Twenty-five rainbow trout, suspected of Enteric Redmouth Disease, were examined in this study. Yersinia ruckeri strains were identified from 8 out of 25 samples (32%). Ten of 25 (40%) rainbow trout samples showed positive reactions for Yersinia ruckeri in the PCR. All PCR positive samples have shown specific amplification bands of the 575-bp fragment for Yersinia ruckeri.

Özet

Bu araştırmada, Gökkuşağı Alabalığı çiftliklerindeki Yersinia ruckeri varlığı Polimeraz Zincir Reaksiyonu (PZR) metodu ile incelendi. Bu çalışmada Kızıl Ağız Hastalığı şüpheli 25 adet Gökkuşağı Alabalığı materyal olarak kullanıldı. 25 örneğin 8 (% 32)'inden Yersinia ruckeri identifıye edildi. 25 örneğin lO'unda PZR'da Yersinia ruckeri yönllnden pozitiflik saptandı. PZR pozitif Ürünlerin hepsinde, Yersinia ruckeri için spesifik 575 bp uzunluğunda bantlar elde edildi.

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Cite This Article

KIRKAN, Ş., GÖKSOY, E. Ö., TEKBIYIK, S., KAYA, O. (2022). Türkiye' nin batısındaki Gökkuşağı alabalık yetiştirme çiftliklerinde Yersinia ruckeri' nin PZR kullanılarak tanımlanması. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 32 - Sayı: 3, 21–30. https://ajindex.com/articles/555286

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