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Journals / Kocatepe Tıp Dergisi / 2026 / Cilt: 26 Sayı: 3

THERAPEUTIC EFFICACY OF DOCETAXEL AND COMBRETASTATIN A4 LOADED PEG-PLGA NANOPARTICLES IN HUMAN BREAST CANCER CELLS

İNSAN MEME KANSERİ HÜCRELERİNDE DOSETAKSEL VE KOMBRETASTATİN A4 YÜKLÜ PEG-PLGA NANOPARTİKÜLLERİNİN TEDAVİ ETKİNLİĞİ

Sadık Kağa(AFYON KOCATEPE ÜNİVERSİTESİ)0000-0002-6303-7981
Didem Kesgin(AFYON KOCATEPE ÜNİVERSİTESİ)0000-0002-0624-2196
Elif Kağa(AFYONKARAHISAR HEALTH SCIENCES UNIVERSITY)0000-0002-2279-6105
Pages
262–271
Publication Date
2025-07-16
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

CONCLUSIONS: The designed nano dual drug formulation can be an alternative chemotherapy solution for cancer treatment.

📌 3 Key Takeaways

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Objective & Hypothesis:OBJECTIVE: Although chemotherapy is widely used in the treatment of cancer, it suffers due to the nonspecific biodistribution and side effects of chemotherapy agents.
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Key Findings & Data:In this study, a nano formulation that can both prevent the formation of drug resistance and increase the effectiveness of treatment was developed.
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Conclusion & Impact:CONCLUSIONS: The designed nano dual drug formulation can be an alternative chemotherapy solution for cancer treatment.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Docetaksel · Kombretastatin A4 · Nanopartikül

Abstract

OBJECTIVE: Although chemotherapy is widely used in the treatment of cancer, it suffers due to the nonspecific biodistribution and side effects of chemotherapy agents. In this study, a nano formulation that can both prevent the formation of drug resistance and increase the effectiveness of treatment was developed. MATERIAL AND METHODS: Docetaxel (DTX) and Combretastatin A4 (CA4) loaded PEG-PLGA (Poly(ethylene glycol)-block-Poly(lactide-co-glycolide) polymeric nanoparticles were fabricated with mono and binary drug loading. The size and morphology of the nanoparticles were characterized using Transmission Electron Microscopy (TEM). The UV-Vis spectrometer determined critical Micelle Concentration (CMC) values, drug encapsulation efficiency, and drug release profiles of the mono and binary drug-loaded nanoparticles. In vitro efficiency of the DTX and CA4 loaded PEG-PLGA nanoparticles was tested using a human breast cancer cell line (MCF-7) compared to free drug formulations. RESULTS: PEG-PLGA nanoparticles had a CMC value of 0.0126 mM. The size of the particles was measured at around 5-10 nm. They released almost all loaded content in 4 days at pH 5.5, whereas this value remained 60% at pH 7.4. DTX and CA4 loaded nanoparticles showed a lower EC50 value than the dual free drug formulation. CONCLUSIONS: The designed nano dual drug formulation can be an alternative chemotherapy solution for cancer treatment.

Özet

AMAÇ: Kemoterapi kanser tedavisinde yaygın olarak kullanılmasına rağmen, kemoterapi ajanlarının nonspesifik biyodağılımları ve yan etkileri nedeniyle problemler mevcuttur. Bu çalışmada, hem ilaç direncinin oluşumunu önleyebilen hem de tedavinin etkinliğini artırabilen bir nanoformülasyon geliştirilmiştir. GEREÇ VE YÖNTEM: Docetaxel (DTX) ve Combretastatin A4 (CA4) yüklü PEG-PLGA (Poli(etilen glikol)-blok-Poli(laktid-ko-glikolid) polimerik nanopartiküller mono ve ikili ilaç yüklemesi ile üretildi. Nanopartiküllerin boyutu ve morfolojisi Transmisyon Elektron Mikroskobu (TEM) kullanılarak karakterize edildi. Tekli ve ikili ilaç yüklü nanopartiküllerin kritik misel konsantrasyonu (CMC) değerleri, ilaç kapsülleme verimliliği ve ilaç salım profilleri UV-Vis spektrometresi ile belirlendi. DTX ve CA4 yüklü PEG-PLGA nanopartiküllerinin in vitro etkinliği, serbest ilaç formülasyonlarıyla karşılaştırılarak, insan meme kanseri hücre hattında (MCF-7) test edildi. BULGULAR: PEG-PLGA nanopartiküllerine ait CMC değeri 0,0126 mM olarak bulunmuştur. Partikül boyutları yaklaşık 5–10 nm aralığında ölçülmüştür. Nanopartiküller, pH 5,5 koşullarında, 4 gün içinde neredeyse tüm yüklü içeriği serbest bırakırken, pH 7,4 ortamında bu oran %60 seviyesinde kalmıştır. DTX ve CA4 yüklü nanopartiküller, ikili serbest ilaç formülasyonuna kıyasla daha düşük bir EC50 değeri sergilemiştir. SONUÇ: Tasarlanan ikili nano ilaç formülasyonu, kanser tedavisi için alternatif bir kemoterapi çözümü olabilir.

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Cite This Article

Kağa, S., Kesgin, D., Kağa, E. (2025). THERAPEUTIC EFFICACY OF DOCETAXEL AND COMBRETASTATIN A4 LOADED PEG-PLGA NANOPARTICLES IN HUMAN BREAST CANCER CELLS. Kocatepe Tıp Dergisi, Cilt: 26 Sayı: 3, 262–271. https://doi.org/10.18229/kocatepetip.1676373

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