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Journals / Anatolian Bryology / 2025 / Cilt: 11 Sayı: 2

Antimicrobial and Antibiofilm Potentials of Rhynchostegium riparioides (Hedw.) Cardot Extracts

Rhyncostegium riparioides (Hedw.) Cardot Ekstraktlarının Antimikrobiyal ve Antibiyofilm Potansiyelleri

Cenker Yaman(DOKUZ EYLUL UNIVERSITY, INSTITUTE OF SCIENCE)0000-0002-3559-9098
Gülcan Gören(DOKUZ EYLUL UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY)0000-0002-7059-1219
Gizem Gül(DOKUZ EYLUL UNIVERSITY, INSTITUTE OF SCIENCE, BIOLOGY (MASTER))0000-0003-3928-2917
Kutay Orkun Durukan(DOKUZ EYLUL UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY, BIOLOGY PR.)0009-0009-5456-3793
Ezginur Duman(DOKUZ EYLUL UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY, BIOLOGY PR.)0009-0005-4561-2152
Dilay Turu(DOKUZ EYLUL UNIVERSITY, INSTITUTE OF SCIENCE, BIOLOGY (MASTER))0000-0002-8485-0488
Atakan Benek(DOKUZ EYLÜL ÜNİVERSİTESİ, FAUNA VE FLORA ARAŞTIRMA VE UYGULAMA MERKEZİ)0000-0001-6726-5968
Ayşe Dilek Unan(BULENT ECEVIT UNIVERSITY)0000-0003-4812-7871
Mustafa Eray Bozyel(DOKUZ EYLUL UNIVERSITY, FACULTY OF SCIENCE)0000-0001-5556-4880
Kerem Canlı(DOKUZ EYLUL UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY)0000-0001-6061-6948
Pages
83–110
Publication Date
2025-12-12
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The findings underscore the potential for developing the n-hexane extracts as natural antibiofilm agents.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, antimicrobial and antibiofilm activities of extracts obtained from the moss Rhynchostegium riparioides using ethanol, methanol, n-hexane, and water solvents were evaluated.
📊
Key Findings & Data:This extract was predominantly composed (79.29%) of tris(2,4-di-tert-butylphenyl) phosphate.
💡
Conclusion & Impact:The findings underscore the potential for developing the n-hexane extracts as natural antibiofilm agents.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Biyofilm inhibisyonu · antibakteriyel etki · Gaz Kromatografisi-Kütle Spektrometresi (GC-MS).

Abstract

In this study, antimicrobial and antibiofilm activities of extracts obtained from the moss Rhynchostegium riparioides using ethanol, methanol, n-hexane, and water solvents were evaluated. Standard strains, food isolates, and clinical isolates, including multidrug-resistant strains, were utilized in the tests. The efficacy of the extracts was determined using disk diffusion and minimum inhibitory concentration (MIC) methods, and their phytochemical profiles were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The n-hexane extract showed the highest antimicrobial activity, demonstrating strong effects even at the lowest tested concentrations. This extract was predominantly composed (79.29%) of tris(2,4-di-tert-butylphenyl) phosphate. The methanol extract exhibited broad-spectrum activity, notably effective against Enterococcus faecalis and Escherichia coli, and major compounds identified included neophytadiene (21.55%), palmitic acid (18.65%), and linolenic acid (10.45%). In the antibiofilm assays, a strong inhibition of up to 95% was observed, particularly against Listeria innocua. These findings highlight the potential of the n-hexane extract for development as a natural antibiofilm agent. The findings underscore the potential for developing the n-hexane extracts as natural antibiofilm agents.

Özet

Bu çalışmada, Rhynchostegium riparioides karayosunundan etanol, metanol, n-hekzan ve su çözücüleri ile elde edilen ekstraktların antimikrobiyal ve antibiyofilm aktiviteleri değerlendirilmiştir. Testlerde standart suşlar, gıda izolatı ve çoklu ilaç direncine sahip suşların da bulunduğu klinik izolatlar kullanılmıştır. Ekstraktların etkinliği disk difüzyon ve minimum inhibitör konsantrasyon (MİK) yöntemleriyle belirlenmiş, fitokimyasal profilleri ise Gaz Kromatografisi-Kütle Spektrometrisi (GC-MS) ile analiz edilmiştir. En yüksek antimikrobiyal aktiviteyi, test edilen en düşük konsantrasyonlarda bile güçlü etki gösteren ve içeriğinin %79,29’unu tris(2,4-di-tert-bütilfenil) fosfat bileşiğinin oluşturduğu n-hekzan ekstraktı sergilemiştir. Metanol ekstraktı geniş spektrumlu bir etki göstererek özellikle Enterococcus faecalis ve Escherichia coli üzerinde etkili bulunmuş, ekstraktta neofitadien (%21,55), palmitik asit (%18,65) ve linolenik asit (%10,45) gibi majör bileşikler tanımlanmıştır. Antibiofilm testlerinde özellikle Listeria innocua üzerinde %95’e varan güçlü bir inhibisyon gözlenmiştir. Bulgular, özellikle n-hekzan ekstraktının doğal antibiyofilm ajanı olarak geliştirilmesi açısından önem taşımaktadır.

🔖

Cite This Article

Yaman, C., Gören, G., Gül, G., Durukan, K. O., Duman, E., Turu, D., Benek, A., Unan, A. D., Bozyel, M. E., Canlı, K. (2025). Antimicrobial and Antibiofilm Potentials of Rhynchostegium riparioides (Hedw.) Cardot Extracts. Anatolian Bryology, Cilt: 11 Sayı: 2, 83–110. https://doi.org/10.26672/anatolianbryology.1727022

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