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Journals / Anatolian Bryology / 2025 / Cilt: 11 Sayı: 2

Screening of Bioactive Potential of Plasteurhynchium striatulum: Antibiofilm Effects and Phytochemical Profile

Plasteurhynchium striatulum’un Biyoaktif Potansiyelinin Taranması: Antibiyofilm Etkileri ve Fitokimyasal Profili

Gizem Gül(Dokuz Eylül Üniversitesi)0000-0003-3928-2917
Elif Gönül(DOKUZ EYLUL UNIVERSITY)0009-0008-6978-8058
Cenker Yaman(DOKUZ EYLUL UNIVERSITY)0000-0002-3559-9098
Melike Ersin(DOKUZ EYLUL UNIVERSITY)0009-0002-2234-822X
Selime Deniz Bozkurt(DOKUZ EYLUL UNIVERSITY)0009-0003-4782-8884
Gülcan Gören(DOKUZ EYLUL UNIVERSITY)0000-0002-7059-1219
Ezginur Duman(DOKUZ EYLUL UNIVERSITY)0009-0005-4561-2152
Atakan Benek(DOKUZ EYLUL UNIVERSITY)0000-0001-6726-5968
Ayşe Dilek Unan(BULENT ECEVIT UNIVERSITY)0000-0003-4812-7871
Mustafa Eray Bozyel(CANAKKALE ONSEKIZ MART UNIVERSITY)0000-0001-5556-4880
Kerem Canlı(DOKUZ EYLUL UNIVERSITY)0000-0001-6061-6948
Pages
111–130
Publication Date
2025-12-12
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, P. striatulum was found to possess notable antimicrobial and antioxidant activities, along with a rich biochemical composition.

📌 3 Key Takeaways

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Objective & Hypothesis:In the present study, the bryophyte Plasteurhynchium striatulum (Spruce) M.
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Key Findings & Data:The highest MIC value was recorded as 0.8816 mg/mL against the Salmonella infantis strain.
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Conclusion & Impact:In conclusion, P. striatulum was found to possess notable antimicrobial and antioxidant activities, along with a rich biochemical composition.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Bryofit · Antimikrobiyal Aktivite · Antioksidan Aktivite

Abstract

In the present study, the bryophyte Plasteurhynchium striatulum (Spruce) M. Fleisch was extracted using three different solvents: ethanol, methanol, and n-hexane. Subsequently, the antimicrobial activities of the extracts were evaluated against a range of microorganisms including foodborne isolates, clinical isolates, multidrug-resistant strains, and standard strains using disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibiofilm assays. In addition, antioxidant activity was assessed through the DPPH radical scavenging assay. The biochemical constituents of the extracts were identified via gas chromatography–mass spectrometry (GC-MS) analysis. According to the disk diffusion test results, activity was observed against eight bacterial strains, most notably Escherichia coli and Providencia rustigianii, both of which exhibit multidrug resistance. The highest MIC value was recorded as 0.8816 mg/mL against the Salmonella infantis strain. The strongest antioxidant performance was observed at a concentration of 1 mg/mL. GC-MS analysis revealed that the major components of the extract were Tris(2,4-di-tert-butylphenyl) phosphate (52.45%), A'-Neogammacer-22(29)-ene (25.00%), and Neophytadiene (12.72%). In conclusion, P. striatulum was found to possess notable antimicrobial and antioxidant activities, along with a rich biochemical composition. These findings suggest that P. striatulum holds promise as a potential source for the development of novel antimicrobial and antioxidant agents. Additionally, its biochemical characteristics could offer important information for upcoming studies.

Özet

Gerçekleştirilen bu çalışmada bir bryofit olan Plasteurhynchium striatulum (Spruce) M. Fleisch’un etanol, metanol ve n-hekzan olmak üzere üç farklı solvent ile ekstraksiyonu gerçekleştirilmiştir. Ardından gıda izolatları, klinik izolatlar, çoklu ilaca dirençli ve standart suşlar dahil olmak üzere çeşitli mikroorganizmalara karşı antimikrobiyal aktiviteleri disk difüzyon, minimum inhibitör konsantrasyon (MİK), minimum bakterisidal konsantrasyonu (MBC) ve antibiyofilm testleri ile tespit edilmiştir. Bununla birlikte antioksidan aktivitelerinin tespiti DPPH testi ile gerçekleştirilmiştir. Biyokimyasal içerikleri Gaz kromatografisi-kütle spektrometrisi (GC-MS) yöntemi kullanılarak belirlenmiştir. Disk difüzyon testi bulgularında başta çoklu ilaç direncine sahip Escherichia coli ve Providencia rustigianii olmak üzere toplamda 8 bakteride etki gözlemlenmiştir. MİK testinde en yüksek sonuç Salmonella infantis suşunda 0,8816 mg\mL olarak belirlenmiştir. Antioksidan aktivite testinde en yüksek temizleme performansı ise 1 mg/mL konsantrasyonunda gerçekleşmiştir. GC-MS analizi sonucunda majör madde olarak Tris(2,4-di-tert-butylphenyl) phosphate (%52,45), A'-Neogammacer-22(29)-ene (%25.00) ve Neophytadiene (%12,72) tespit edilmiştir. Sonuç olarak, P. striatulum’un antimikrobiyal ve antioksidan aktivitesinin yanı sıra oldukça zengin bir biyokimyasal içeriğe sahip olduğu belirlenmiştir. Bu durum P. striatulum’un gelecekte antimikrobiyal ve antioksidan etkinliğe sahip yeni ajanların oluşturulması hususunda umut verici bir nitelik taşırken biyokimyasal yapısının aydınlatılması gelecekteki çalışmalar için önemli bilgiler sunmaktadır.

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Cite This Article

Gül, G., Gönül, E., Yaman, C., Ersin, M., Bozkurt, S. D., Gören, G., Duman, E., Benek, A., Unan, A. D., Bozyel, M. E., Canlı, K. (2025). Screening of Bioactive Potential of Plasteurhynchium striatulum: Antibiofilm Effects and Phytochemical Profile. Anatolian Bryology, Cilt: 11 Sayı: 2, 111–130. https://doi.org/10.26672/anatolianbryology.1810483

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