Journals / Anadolu Tarım Bilimleri Dergisi / 2026 / Cilt: 39 Sayı: 2

Solid-state Fermentation of Wheat Bran by Aspergillus niger Strains: Effect on the Nutritional Composition and In vitro Digestibility

Buğday Kepeğinin Aspergillus niger Suşları ile Katı Faz Fermantasyonu: Besin Madde Kompozisyonu ve In vitro Sindirilebilirliği Üzerine Etkisi

Aydın Altop(ONDOKUZ MAYIS UNIVERSITY)0000-0002-3966-300X
Emrah Güngör(ONDOKUZ MAYIS UNIVERSITY)0000-0003-4380-6162
Şevket Özlü(ONDOKUZ MAYIS UNIVERSITY)0000-0003-4494-345X
Güray Erener(ONDOKUZ MAYIS UNIVERSITY)0000-0002-8025-2560
Pages
239–246
Publication Date
2024-06-28
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

ATCC 52172 can be recommended for higher CP and ATCC 200345 for lower CF and ADF and in solid-state fermentation of WB.

📌 3 Key Takeaways

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Objective & Hypothesis:This study aimed to investigate the effect of Aspergillus niger on the nutritional composition and in vitro dry matter digestibility of wheat bran (WB) in solid-state fermentation.
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Key Findings & Data:Two A. niger strains (ATCC 200345 or ATCC 52172) were used as inoculants in solid-state fermentation of WB.
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Conclusion & Impact:ATCC 52172 can be recommended for higher CP and ATCC 200345 for lower CF and ADF and in solid-state fermentation of WB.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Fermantasyon · Mantar · Buğday kepeği

Abstract

This study aimed to investigate the effect of Aspergillus niger on the nutritional composition and in vitro dry matter digestibility of wheat bran (WB) in solid-state fermentation. Two A. niger strains (ATCC 200345 or ATCC 52172) were used as inoculants in solid-state fermentation of WB. Wheat bran was sterilized at 121°C for 15 min and inoculated with A. niger strains at 105 spores kg-1 WB. Samples were incubated at 60°C for two days and dried at room temperature until reaching 90% dry matter. Crude protein (CP), ether extract (EE), ash, crude fiber (CF), hemicellulose, neutral detergent fiber (NDF) and acid detergent fiber (ADF) content and in vitro dry matter digestibility of WB and fermented WB were determined. The CP and ash levels were increased (P<0.001) by solid-state fermentation using A. niger strains. However, both A. niger strains decreased (P<0.001) the CF, NDF, ADF, and hemicellulose content of WB. The EE content of WB was reduced (P=0.027) by ATCC 200345 but was not affected (P=0.145) by ATCC 52172. The in vitro dry matter digestibility of WB was increased (P=0.013) by both A. niger strains. The greater increases in CP and ash content and the greater decrease in hemicellulose were obtained from ATCC 52172 (P<0.001). However, greater decreases in CF and ADF content were observed in fermented WB with ATCC 200345 compared to fermented WB with ATCC 52172 (P=0.037 and P=0.013, respectively). The results of the present study showed that A. niger improved the nutritional composition and in vitro dry matter digestibility of WB with solid-state fermentation. ATCC 52172 can be recommended for higher CP and ATCC 200345 for lower CF and ADF and in solid-state fermentation of WB.

Özet

Bu çalışmada, buğday kepeğinin (BK) besin madde kompozisyonu ve in vitro kuru madde sindirilebilirliği üzerine Aspergillus niger kullanılan katı faz fermantasyonun etkileri araştırılmıştır. BK’nin katı faz fermantasyonunda iki farklı A. niger suşu (ATCC 200345 ve ATCC 52172) inokulant olarak kullanılmıştır. BK, 121°C’de 15 dakika steril edilmiş ve A. niger suşları 105 spor kg-1 BK düzeyinde inokule edilmiştir. Örnekler 60°C’de 2 gün inkübe edilmiş ve %90 kuru maddeye ulaşana kadar oda sıcaklığında kurutulmuştur. BK ve fermente BK’nin, ham protein (HP), ham yağ (HY), ham kül (HK), ham selüloz (HS), hemiselüloz, nötr deterjanda çözünmeyen lif (NDF), asit deterjanda çözünmeyen lif (ADF) düzeyleri ile in vitro kuru madde sindirilebilirliği belirlenmiştir. BK’nin HP ve HK düzeyleri, A. niger suşlarının kullanıldığı katı faz fermantasyonu ile artmıştır (P<0.001). Buna karşın, her iki A. niger suşu da BK’nin HS, NDF, ADF ve hemiselüloz düzeylerini azaltmıştır (P<0.001). BK'nin HY içeriği, ATCC 200345 ile azaltılırken (P=0.027) ATCC 52172’den etkilenmemiştir (P=0.145). BK’nin in vitro kuru madde sindirilebilirliği her iki A. niger suşu ile artırılmıştır (P=0.013). BK’nin HP ve HK düzeyindeki en büyük artış ile hemiselüloz düzeyindeki en büyük düşüş ATCC 52172’den elde edilmiştir (P<0.001). Bununla birlikte, ATCC 200345 kullanılarak fermente edilen BK’nin HS ve ADF düzeyleri ATCC 52172 ile fermente edilen BK’den daha düşük olmuştur (sırasıyla P=0.037 ve P<0.013). Çalışmanın sonuçları, A. niger kullanılan katı faz fermantasyonu ile BK’nin besin madde kompozisyonunun ve in vitro kuru madde sindirilebilirliğinin iyileştirilebileceğini göstermiştir. BK’nin katı kültür fermantasyonunda daha yüksek HP içeriği için ATCC 52172, daha düşük HS ve ADF içeriği için ATCC 200345 önerilebilir.

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Cite This Article

Altop, A., Güngör, E., Özlü, Ş., Erener, G. (2024). Solid-state Fermentation of Wheat Bran by Aspergillus niger Strains: Effect on the Nutritional Composition and In vitro Digestibility. Anadolu Tarım Bilimleri Dergisi, Cilt: 39 Sayı: 2, 239–246. https://doi.org/10.7161/omuanajas.1314081

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References (17)

  1. Altop, A., Güngör, E., Erener, G., 2019. Improvement of nutritional quality of some oilseed meals through solid-state fermentation using Aspergillus niger. Turkish Journal of Agriculture-Food Science and Technology, 7(9): 1411-4. doi:10.24925/turjaf.v7i9.1411-1414.2721
  2. AOAC. Official Methods of Analysis of AOAC International (17. Edition). ABD: AOAC International; 2000.
  3. Couri, S., da Costa Terzi, S., Pinto, G.A.S., Freitas, S.P., da Costa, A.C.A., 2000. Hydrolytic enzyme production in solid-state fermentation by Aspergillus niger 3T5B8. Process Biochemistry, 36(3): 255-61.
  4. de Castro, R.J.S., Ohara, A., Nishide, T.G., Bagagli, M.P., Dias, F.F.G., Sato, H.H., 2015. A versatile system based on substrate formulation using agroindustrial wastes for protease production by Aspergillus niger under solid state fermentation. Biocatalysis and Agricultural Biotechnology, 4(4): 678-84.
  5. Gallardo, C., Dadalt, J.C., Trindade Neto, M.A., 2018. Nitrogen retention, energy, and amino acid digestibility of wheat bran, without or with multicarbohydrase and phytase supplementation, fed to broiler chickens. Journal of Animal Science, 96(6): 2371-9.
  6. Gungor, E., Erener, G., 2020. Effect of dietary raw and fermented sour cherry kernel (Prunus cerasus L.) on digestibility, intestinal morphology and caecal microflora in broiler chickens. Poultry Science, 99(1): 471-8.
  7. Güngör, E., Altop, A., Erener, G., 2020. Improvement in the nutritional composition and nutrient digestibility of pomegranate (Punica granatum L.) seed by Bacillus subtilis and Aspergillus niger solid-state fermentation. Anadolu Tarım Bilimleri Dergisi, 35(3): 268-73.
  8. Güngör, E., Altop, A., Öztürk, E., Erener, G., 2017. Nutritional changes of sour cherry (Prunus cerasus) kernel subjected to Aspergillus niger solid-state fermentation. Journal of Tekirdag Agricultural Faculty: 99-103.
  9. Harimurti, S., Hadisaputro, W. Probiotics in poultry. In: Liong M-T, editor. Beneficial microorganisms in agriculture, aquaculture and other areas. Switzerland: Springer Cham; 2015. p. 1-19.
  10. Hui, L., Wan, C., Hai-Tao, D., Xue-Jiao, C., Qi-Fa, Z., Yu-Hua, Z., 2010. Direct microbial conversion of wheat straw into lipid by a cellulolytic fungus of Aspergillus oryzae A-4 in solid-state fermentation. Bioresource Technology, 101(19): 7556-62.
  11. Imelda, J., Paulraj, R., Bhatnagar, D., 2008. Effect of solid state fermentation on nutrient composition of selected feed ingredients. Indian Journal of Fisheries, 55(4): 327-32.
  12. Kang, S., Park, Y., Lee, J., Hong, S., Kim, S., 2004. Production of cellulases and hemicellulases by Aspergillus niger KK2 from lignocellulosic biomass. Bioresource Technology, 91(2): 153-6.
  13. Shang, Q., Wu, D., Liu, H., Mahfuz, S., Piao, X., 2020. The impact of wheat bran on the morphology and physiology of the gastrointestinal tract in broiler chickens. Animals, 10(10): 1831.
  14. Van Soest, P.v., Robertson, J., Lewis, B., 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10): 3583-97.
  15. Xiao, Y., Huang, Y., Chen, Y., Fan, Z., Chen, R., He, C., Li, Z., Wang, Y., 2021. Effects of solid-state fermentation with Eurotium cristatum YL-1 on the nutritional value, total phenolics, isoflavones, antioxidant activity, and volatile organic compounds of black soybeans. Agronomy, 11(6): 1029.
  16. Yegani, M., Swift, M., Zijlstra, R., Korver, D., 2013. Prediction of energetic value of wheat and triticale in broiler chicks: A chick bioassay and an in vitro digestibility technique. Animal Feed Science and Technology, 183(1-2): 40-50.
  17. Zhang, W.-j., Xu, Z.-r., Sun, J.-y., Yang, X., 2006. Effect of selected fungi on the reduction of gossypol levels and nutritional value during solid substrate fermentation of cottonseed meal. Journal of Zhejiang University-Science B, 7(9): 690-5.