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Journals / Anadolu Tarım Bilimleri Dergisi / 2026 / Cilt: 41

The Biocontrol Potential of Trichoderma afroharzianum for Resilience to Verticillium Wilt in Cotton Plants Under Saline Stress

Tuz Stresi Altındaki Pamuk Bitkilerinde Trichoderma afroharzianum'un Verticillium Solgunluğuna Karşı Biyokontrol Potansiyeli

Melisa Kökce(AYDIN ADNAN MENDERES ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, BİTKİ KORUMA BÖLÜMÜ)0009-0009-4867-4550
Yunus Korkom(ADNAN MENDERES UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF PLANT PROTECTION)0000-0001-5859-9026
Pages
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Publication Date
2026-02-27
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Salt-induced changes in both hydraulic and chemical properties of cotton were effected on boll biomass may lead to a loses of sustained yield.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Verticillium dahliae is one of the most important plant pathogens in cotton.
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Key Findings & Data:Results in Petri dishes showed that the three T. afroharzianum isolates (Tr125, Tr28 and Tr55) were determined to be tolerant to high salt concentration (250 mM).
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Conclusion & Impact:Salt-induced changes in both hydraulic and chemical properties of cotton were effected on boll biomass may lead to a loses of sustained yield.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Gossypium hirsutum · Trichoderma · Verticillium dahliae

Abstract

Verticillium dahliae is one of the most important plant pathogens in cotton. This study was aimed to evaluate the effects of Trichoderma afroharzianum, and T. guizhouense treatments on Verticillium wilt in cotton plants under saline stress conditions. Results in Petri dishes showed that the three T. afroharzianum isolates (Tr125, Tr28 and Tr55) were determined to be tolerant to high salt concentration (250 mM). The spore suspension of T. afroharzianum (1×108 conidia ml-1 soil) was previously inoculated in mixture of sterile soil and sand. V. dahliae (1×106 conidia g-1 soil) was infected simultaneously with cotton seed sowing. Sodium chloride (NaCl) was applied to the pots by adding 100 mM through irrigation when the cotton seedlings growth two true leaves. The phenotypic development of the treated cotton plants was monitored over three months. Also, the disease incidence of V.dahliae with salinity in cotton seedling were investigated. Results of pot trials showed that T. afroharzianum Tr125 exhibited the highest disease prevention rate (79%) against V.dahliae as compared to the other isolates under salinity stress. There was an increase in plant fresh weight (15.61 g/plant), root weight (3.52 g/plant), root length (17.67 cm/plant) in treatments with Tr125 as compared to the control of containing NaCl. The highest plant length was determined in T. afroharzianum Tr28 (26.80 cm/plant). The results of the our study demonstrate that Trichoderma afroharzianum Tr125 have potential for the management of Verticillium wilt disease under saline soils when cultivating cotton. As a result, enhancing tolerance to Verticillium wilt under salt stress can be achieved by Trichoderma treatment added with cultural pratices can boost the controlling strategies that improvement the parameters of plant growth. Salt-induced changes in both hydraulic and chemical properties of cotton were effected on boll biomass may lead to a loses of sustained yield.

Özet

Pamukta Verticillium dahliae en önemli bitki patojenlerinden biridir. Bu çalışmada, tuzluluk stresi koşullarındaki pamuk bitkilerine Trichoderma afroharzianum ve T. guizhouense uygulamalarının Verticillium solgunluğuna etkilerinin değerlendirilmesi amaçlanmıştır. Petri kabı deneyi sonucunda üç T. afroharzianum izolatının (Tr125, Tr28 ve Tr55) yüksek tuz konsantrasyonuna (250 mM) tolerant olduğu belirlenmiştir. Saksı denemelerinde, ilk önce T. afroharzianum spor süspansiyonu (1×108 konidi ml-1 toprak) steril toprak ve kum karışımına inokule edilmiştir. Pamuk tohumu ekimi ile eş zamanlı olarak V. dahliae (1×106 konidi g-1 toprak) enfekte edilmiştir. Pamuk fideleri iki gerçek yaprak oluşturduğunda 100 mM sodyum klorür (NaCl) saksılara sulama yoluyla uygulanmıştır. Uygulama yapılan pamuk bitkilerinin fenotipik gelişimi üç ay boyunca gözlenmiştir. Ayrıca tuzluluğa bağlı olarak pamuk fidelerinde V. dahliae hastalığının görülme sıklığı araştırılmıştır. Saksı denemelerinin sonuçları, tuzluluk stresi altında T. afroharzianum Tr125'in diğer izolatlarla karşılaştırıldığında V. dahliae'nin en yüksek hastalık engelleme oranı (%79) gösterdiğini ortaya koymuştur. Tr125 uygulaması tuz içeren kontrole göre karşılaştırıldığında, bitki yaş ağırlığında (15.61 g/bitki), kök ağırlığında (3.52 g/bitki), kök uzunluğunda (17.67 cm/bitki) artış göstermiştir. T. afroharzianum Tr28’de en yüksek bitki boyu elde edilmiştir (26.80 cm/bitki). Çalışmamızın sonuçları, Trichoderma afroharzianum Tr125'in tuzlu topraklarda pamuk yetiştiriciliğinde Verticillium solgunluk hastalığının mücadelesinde potansiyele sahip olduğunu göstermiştir. Sonuç olarak, Trichoderma ile birlikte kültürel uygulamaların gerçekleştirilmesi, tuz stresi altında Verticillium solgunluğuna karşı bitki toleransının artırılmasını sağlayarak bitki gelişim parametrelerini iyileştirmesi kontrol stratejileri güçlendirilebilir. Pamukta tuz düzeyine bağlı olarak hem hidrolik hem de kimyasal özelliklerindeki değişimlerin koza biyokütlesi üzerinde etkili olduğu ve sürdürülebilir verim kayıplarına yol açabileceği düşünülmektedir.

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Cite This Article

Kökce, M., Korkom, Y. (2026). The Biocontrol Potential of Trichoderma afroharzianum for Resilience to Verticillium Wilt in Cotton Plants Under Saline Stress. Anadolu Tarım Bilimleri Dergisi, Cilt: 41. https://doi.org/10.7161/omuanajas.1783185

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