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Journals / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 2026 / Cilt: 47 Sayı: 2

COMPARISON OF SOME BIOLOGICAL ACTIVITIES AND CATECHIN TANNIN CONTENTS OF TWO JUNIPERUS AND PRUNUS SPECIES

İKİ JUNİPERUS VE PRUNUS TÜRÜNÜN BAZI BİYOLOJİK AKTİVİTELERİNİN VE KATEŞİN TANEN İÇERİKLERİNİN KARŞILAŞTIRILMASI

Hafize Yuca(ATATÜRK ÜNİVERSİTESİ)0000-0002-0857-4776
Hakkı Cem Demircan(ATATÜRK ÜNİVERSİTESİ)0000-0001-8336-4552
Bilge Aydın(ERZINCAN BINALI YILDIRIM UNIVERSITY)0000-0002-7712-7259
Mehmet Önal(ATATÜRK ÜNİVERSİTESİ)0000-0003-1069-1012
Enes Tekman(ATATÜRK ÜNİVERSİTESİ)0000-0002-1226-7218
Ayşe Civaş(IĞDIR ÜNİVERSİTESİ)0000-0002-8838-3856
Mohaddeseh Nobarirezaeyeh(ATATÜRK ÜNİVERSİTESİ)0000-0002-2722-6910
Gamze Göger(AFYON KOCATEPE UNIVERSITY)0000-0003-2978-5385
Songül Karakaya(Atatürk Üniversitesi)0000-0002-3268-721X
Zuhal Güvenalp(ATATÜRK ÜNİVERSİTESİ)0000-0002-8803-8147
Pages
650–666
Publication Date
2023-05-20
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this research, it can be said that JCS with the highest tannin content shows the highest effects.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Qualitative and quantitative determination of catechin in fruits of J. communis var. saxatilis, J. oxycedrus subsp. oxycedrus, P. spinosa, and P. cerasifera was analyzed by LC-MS/MS using standards.
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Key Findings & Data:Result and Discussion: JCS (IC50 = 578 ± 20 μg/ml), JOO (IC50 = 3706 ± 52 μg/ml), and PS (IC50 = 313 ± 7 μg/ml) extracts were observed to show a higher activity than acarbose (IC50 = 4061 ± 146 μg/ml).
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Conclusion & Impact:In this research, it can be said that JCS with the highest tannin content shows the highest effects.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antidiyabetik · antimikrobiyal · kateşik

Abstract

Objective: Qualitative and quantitative determination of catechin in fruits of J. communis var. saxatilis, J. oxycedrus subsp. oxycedrus, P. spinosa, and P. cerasifera was analyzed by LC-MS/MS using standards. Antidiabetic, antimicrobial, and antioxidant activities of fruit and cone extracts of these plants were evaluated. Qualitative analysis of secondary metabolites was also done. Material and Method: Antimicrobial activity was done with MIC. ABTS·+ and DPPH• scavenging activies were used antioxidant activity. α-Amylase and α-glucosidase inhibition assays were done for antidiabetic activity. Result and Discussion: JCS (IC50 = 578 ± 20 μg/ml), JOO (IC50 = 3706 ± 52 μg/ml), and PS (IC50 = 313 ± 7 μg/ml) extracts were observed to show a higher activity than acarbose (IC50 = 4061 ± 146 μg/ml). J. communis var. saxatilis fruit extract was observed with MIC values between (312.5 -2500 µg/ml) against all microorganisms. JCS extract has the highest phenolic composition and JOO has the lowest phenolic composition (µg GAE/ mg extract). JCS extract indicated the highest antioxidant activity. It was determined that plant containing the highest catechin (1173.3±5.77 ng/ml) and epigallocatechin (208±7.21 ng/ml) was JCS. In this research, it can be said that JCS with the highest tannin content shows the highest effects.

Özet

Amaç: J. communis var. saxatilis, J. oxycedrus subsp. oxycedrus, P. spinosa, ve P. cerasifera meyvelerinde standartlar kullanılarak LC-MS/MS ile analiz edilmiştir. Bitkilerin meyve ve kozalak ekstrelerinin antidiyabetik, antimikrobiyal ve antioksidan aktiviteleri değerlendirilmiştir. Sekonder metabolitlerin kalitatif analizi de yapılmıştır. Gereç ve Yöntem: MIC ile antimikrobiyal aktivite yapıldı. ABTS·+ ve DPPH• süpürücü testler antioksidan aktiviteler için kullanılmıştır. α-Amilaz ve α-glukosidaz inhibisyon testleri antidiyabetik aktivite için kullanılmıştır. Sonuç ve Tartışma: JCS (IC50 = 578 ± 20 μg/ml), JOO (IC50 = 3706 ± 52 μg/ml) ve PS (IC50 = 313 ± 7 μg/ml) ekstraktlarının akarbozdan (IC50 = 4061 ± 146 μg/ml) daha yüksek aktivite gösterdiği gözlemlenmiştir. Juniperus communis var. saxatilis meyve ekstresi tüm mikroorganizmalara karşı (312.5 -2500 µg/ml) arasında MİK değerleri göstermiştir. JCS en yüksek fenolik bileşime ve JOO en düşük fenolik bileşime sahiptir (µg GAE/ mg ekstrakt). JCS ekstresi en yüksek antioksidan aktiviteyi göstermiştir. En yüksek kateşin (1173.3±5.77 ng/ml) ve epigallokateşin (208±7.21 ng/ml) içeren bitki JCS’dir. Bu araştırmada en yüksek tanen içeriğine sahip JCS’nin en yüksek etkileri gösterdiği söylenebilir.

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Cite This Article

Yuca, H., Demircan, H. C., Aydın, B., Önal, M., Tekman, E., Civaş, A., Nobarirezaeyeh, M., Göger, G., Karakaya, S., Güvenalp, Z. (2023). COMPARISON OF SOME BIOLOGICAL ACTIVITIES AND CATECHIN TANNIN CONTENTS OF TWO JUNIPERUS AND PRUNUS SPECIES. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 47 Sayı: 2, 650–666. https://doi.org/10.33483/jfpau.1218845

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