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Journals / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 2026 / Cilt: 48 Sayı: 3

DETERMINATION OF ANTIVIRAL-DRUG FAVIPIRAVIR FROM BIOLOGICAL SAMPLES BY USING MOLECULAR IMPRINTED POLYMER-BASED ELECTROCHEMICAL SENSOR

BİYOLOJİK ÖRNEKLERDEN MOLEKÜLER BASKILI POLİMER TABANLI ELEKTROKİMYASAL SENSÖR KULLANILARAK ANTİVİRAL-İLAÇ FAVİPİRAVİRİN TAYİNİ

Çiğdem Kanbeş Dindar(ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, TEMEL ECZACILIK BİLİMLERİ BÖLÜMÜ, ANALİTİK KİMYA ANABİLİM DALI)0000-0002-3557-5029
Bengi Uslu(ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, TEMEL ECZACILIK BİLİMLERİ BÖLÜMÜ, ANALİTİK KİMYA ANABİLİM DALI)0000-0002-7327-4913
Pages
1089–1097
Publication Date
2024-09-10
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The detection limit of MIP@o-PD/GCE was obtained to be 1.80 pM, whereas the quantification limit was found to be 6.23 pM.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The molecularly imprinted polymer (MIP) technique was applied in this study for selective, fast, and sensitive electrochemical determination of antiviral drug favipiravir (FAVI).
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Key Findings & Data:For the removal and rebinding procedures, as well as the optimization of conditions and performance measurement of MIP@o-PD/GCE, differential pulse voltammetry (DPV) was used.
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Conclusion & Impact:The detection limit of MIP@o-PD/GCE was obtained to be 1.80 pM, whereas the quantification limit was found to be 6.23 pM.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Antiviral ilaç · elektroanaliz · elektrokimyasal sensör

Abstract

Objective: The molecularly imprinted polymer (MIP) technique was applied in this study for selective, fast, and sensitive electrochemical determination of antiviral drug favipiravir (FAVI). Material and Method: By electropolymerizing the functional monomer o-phenylenediamine (o-PD) on a glassy carbon electrode (GCE) in the presence of a template molecule FAVI, the new MIP-based sensor (MIP@o-PD/GCE) was constructed using cyclic voltammetry (CV). For the removal and rebinding procedures, as well as the optimization of conditions and performance measurement of MIP@o-PD/GCE, differential pulse voltammetry (DPV) was used. The ferrocyanide/ferricyanide redox marker was used to monitor each step of the experimental procedure using DPV. Result and Discussion: MIP@o-PD/GCE has a linear response to FAVI in the range from 10 pM to 90 pM under optimal experimental conditions for human serum samples. The detection limit of MIP@o-PD/GCE was obtained to be 1.80 pM, whereas the quantification limit was found to be 6.23 pM. The designed sensor was successfully applied to a synthetic human serum sample to verify its applicability and validity. Electrochemical sensor selectivity was evaluated by comparing the binding of paracetamol and tenofovir, which are similar to favipiravir, and also oseltamivir and famciclovir, which are other drugs used in the treatment of COVID-19.

Özet

Amaç: Bu çalışmada moleküler baskılanmış polimer (MIP) tekniği, antiviral ilaç favipiravirin (FAVI) seçici, hızlı ve hassas elektrokimyasal tayini için uygulanmıştır. Gereç ve Yöntem: Döngüsel voltammetri (CV) kullanılarak, fonksiyonel monomer o-fenilendiaminin (o-PD) bir şablon molekül FAVI varlığında camsı karbon elektrot (GCE) üzerinde elektropolimerize edilmesiyle, yeni MIP tabanlı sensör (MIP@o-PD/GCE) oluşturulmuştur. MIP@o-PD/GCE'nin uzaklaştırma ve yeniden bağlama prosedürlerinin yanı sıra etki edebilecek koşulların optimizasyonu ve performans ölçümü için diferansiyel puls voltametrisi (DPV) kullanılmıştır. Ferrosiyanid/ferrisiyanid redoks işaretleyicisi, DPV kullanılarak deneysel prosedürün her adımını izlemek için kullanılmıştır. Sonuç ve Tartışma: İnsan serum numune örnekleri için MIP@o-PD/GCE, optimum deneysel koşullar altında 10 pM ila 90 pM aralığında FAVI’e doğrusal bir yanıt vermektedir. MIP@o-PD/GCE'nin tespit limiti 1.80 pM olarak elde edilirken, miktar belirleme limiti 6.23 pM olarak bulunmuştur. Tasarlanan sensör, uygulanabilirliğini ve geçerliliğini doğrulamak için sentetik bir insan serumu örneğine başarıyla uygulanmıştır. Elektrokimyasal sensör seçiciliği, FAVI ile benzerlik gösteren parasetamol ve tenofovir ile Covid-19 tedavisinde kullanılan diğer ilaçlar olan oseltamivir ve famsiklovirin bağlanması karşılaştırılarak değerlendirilmiştir.

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Cite This Article

Dindar, Ç. K., Uslu, B. (2024). DETERMINATION OF ANTIVIRAL-DRUG FAVIPIRAVIR FROM BIOLOGICAL SAMPLES BY USING MOLECULAR IMPRINTED POLYMER-BASED ELECTROCHEMICAL SENSOR. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 48 Sayı: 3, 1089–1097. https://doi.org/10.33483/jfpau.1522582

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