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Journals / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 2026 / Cilt: 49 Sayı: 3

ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ENDEMIC HAPLOPHYLLUM MYRTIFOLIUM BOISS.

ENDEMİK HAPLOPHYLLUM MYRTIFOLIUM BOİSS.'UN ANTİMİKROBİYAL VE ANTİOKSİDAN AKTİVİTESİ

Nagehan Saltan(ANADOLU UNIVERSITY, FACULTY OF PHARMACY)0000-0002-1207-909X
Fatmanur Tunç(ANADOLU ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0009-0004-1451-1001
Zeynep Gülcan(ANADOLU UNIVERSITY, FACULTY OF PHARMACY)0000-0002-4379-3253
Pervin Soyer(ANADOLU UNIVERSITY, FACULTY OF PHARMACY)0000-0002-6258-1993
Yavuz Bülent Köse(ANADOLU UNIVERSITY, FACULTY OF PHARMACY)0000-0002-3060-7271
Pages
852–858
Publication Date
2025-09-19
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The minimum bactericidal concentrations of the H. myrtifolium methanol extracts varied between 0.125-2 mg/ml and the n-hexane extracts showed antimicrobial activity with the 3 mg/ml concentration.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Haplophyllum myrtifolium Boiss. an endemic species for Türkiye, belongs to the Rutaceae family.
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Key Findings & Data:The antibacterial effects of H. myrtifolium extracts were assayed against Stapylococcus aureus ATCC 29213, Bacillus subtilis NRRL B478 and Enterecoccus faecalis ATCC 51299 by the broth microdilution method.
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Conclusion & Impact:The minimum bactericidal concentrations of the H. myrtifolium methanol extracts varied between 0.125-2 mg/ml and the n-hexane extracts showed antimicrobial activity with the 3 mg/ml concentration.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antimikrobiyal aktivite · antioksidan aktivite · Haplophyllum myrtifolium

Abstract

Objective: Haplophyllum myrtifolium Boiss. an endemic species for Türkiye, belongs to the Rutaceae family. In this study, antioxidant and antimicrobial effects of n-hexane and methanol extracts of the aerial parts were examined. Material and Method: The total phenolic content of the extracts was examined by a spectrophotometric method using Folin-Ciocalteu reagent (FCR). The DPPH free radical scavenging and ABTS test were applied to determine the antioxidant effect. The antibacterial effects of H. myrtifolium extracts were assayed against Stapylococcus aureus ATCC 29213, Bacillus subtilis NRRL B478 and Enterecoccus faecalis ATCC 51299 by the broth microdilution method. Result and Discussion: The total phenolic content was found to be 44.69±0.021 mg GAE/g for the methanol extract. The methanol extract of H. myrtifolium exhibited higher antioxidant activity (IC50 0.120±0.0008 mg/ml) compared to the n-hexane extract (IC50 0.256±0.015 mg/ml). ABTS test results, the data closest to the TEAC standard was seen in methanol extract (1.783±0.0062 mM). The minimum bactericidal concentrations of the H. myrtifolium methanol extracts varied between 0.125-2 mg/ml and the n-hexane extracts showed antimicrobial activity with the 3 mg/ml concentration.

Özet

Amaç: Haplophyllum myrtifolium Boiss. Türkiye'ye özgü bir tür olup, Rutaceae familyasına aittir. Bu çalışmada, toprak üstü kısımlarının metanol ve n-hekzan ekstrelerinin antimikrobiyal ve antioksidan etkileri araştırılmıştır. Gereç ve Yöntem: Toplam fenol içeriği, gallik asit eşdeğeri (GAE) olarak Folin-Ciocalteu reaktifi (FCR) kullanılarak belirlenmiştir. Antioksidan etkiyi belirlemek için DPPH ve ABTS testi uygulanmıştır. H. myrtifolium ekstrelerinin antibakteriyel etkileri Stapylococcus aureus ATCC 29213, Bacillus subtilis NRRL B478 ve Enterecoccus faecalis ATCC 51299'a karşı brot mikrodilüsyon yöntemiyle test edilmiştir. Sonuç ve Tartışma: Metanol özütü için toplam fenolik içerik 44.69±0.021 mg GAE/g olarak bulundu. H. myrtifolium metanol özütü, n-hekzan özütüne (IC50 0.256±0.015 mg/ml) kıyasla daha yüksek antioksidan aktivite (IC50 0.120±0.0008 mg/ml) gösterdi. ABTS test sonuçlarına göre TEAC standardına en yakın veri metanol ekstrelerinde (1.783±0.0062 mM) görüldü. H. myrtifolium metanol ekstrelerinin minimum bakterisidal konsantrasyonları 0.125-2 mg/ml arasında değişirken, n-hekzan ekstreleri 3 mg/ml konsantrasyonda antimikrobiyal aktivite göstermiştir.

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Cite This Article

Saltan, N., Tunç, F., Gülcan, Z., Soyer, P., Köse, Y. B. (2025). ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ENDEMIC HAPLOPHYLLUM MYRTIFOLIUM BOISS.. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 49 Sayı: 3, 852–858. https://doi.org/10.33483/jfpau.1629137

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References (37)

  1. World Flora Online Web site. (2025). From https://www.worldfloraonline.org/search?query=rutaceae&limit=24&start=0&facet=taxon.family_ss%3ARutaceae&sort=. Accessed date: 28.01.2025.
  2. Bizim bitkiler web site. (2013). From https://www.bizimbitkiler.org.tr/list.html. Accessed date: 04.08.2025.
  3. Davis, P.H., Mill, R.R., Kit, T. (1988). Flora of Turkey and the East Aegean Islands (Volume 10), Edinburgh University Press, Edinburgh, p.496.
  4. Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler), Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul, p.835.
  5. Tugay, O., Ulukuş, D. (2017). Haplophyllum sahinii (Rutaceae), a new species from Central Anatolia (Turkey). Phytotaxa, 297(3), 265-272. [CrossRef]
  6. Ulukuş, D., Tugay, O. (2018). Haplophyllum ermenekense (Rutaceae), a new species from Turkey. PhytoKeys, 111, 119-131. [CrossRef]
  7. Townsend, C.C. (1967). Haplophyllum A. Juss. In: P.H. Davis (Ed.), Flora of Turkey and the East Aegean Islands, (pp.496-506). Edinburgh: Edinburgh University Press.
  8. Ulubelen, A., Öztürk, M. (2008). Alkaloids, coumarins and lignans from Haplophyllum species. Records of Natural Products, 2(3), 54-69.
  9. Al-Snafi, A.E. (2018). Pharmacological importance of Haplophyllum species grown in Iraq-A review. IOSR Journal of Pharmacy, 8(5), 54-62.
  10. Mohammadhosseini, M., Venditti, A., Frezza, C., Serafini, M., Bianco, A., Mahdavi, B. (2021). The genus Haplophyllum Juss.: Phytochemistry and bioactivities-A review. Molecules, 26(15), 4664. [CrossRef]
  11. Tekin, M., Eruygur, N. (2016). The structural studies on the medicinal plant Haplophyllum telephioides. Revista Brasileira de Farmacognosia, 26(5), 544-552. [CrossRef]
  12. Hadjadj, S., Bayoussef, Z., El Hadj-Khelil, A.O., Beggat, H., Bouhafs, Z., Boukaka, Y., Tey, M. (2015). Ethnobotanical study and phytochemical screening of six medicinal plants used in traditional medicine in the Northeastern Sahara of Algeria (area of Ouargla). Journal of Medicinal Plants Research, 9(41), 1049-1059. [CrossRef]
  13. Batanouny, K.H., Abou Tabl, E., Shabana, M., Soliman, F. (1999). Wild Medicinal Plants in Egypt (Volume 154), The Palm Press, Cairo, pp.1-207.
  14. Ghorbani, A. (2005). Studies on pharmaceutical ethnobotany in the region of Turkmen Sahra, north of Iran:(Part 1): General results. Journal of Ethnopharmacology, 102(1), 58-68. [CrossRef]
  15. Mossa, J.S., Al-Yahya, M.A., Al-Meshal, I.A. (1987). Medicinal Plants of Saudi Arabia (Volume 1), King Saud University, Libraries Publications Riyadh, KSA, p.340.
  16. Al-Yahya, M.A., Al-Rehaily, A.J., Ahmad, M.S., Al-Said, M.S., El-Feraly, F.S., Hufford, C.D. (1992). New alkaloids from Haplophyllum tuberculatum. Journal of Natural Products, 55(7), 899-903. [CrossRef]
  17. Raissi, A., Arbabi, M., Roustakhiz, J., Hosseini, M. (2016). Haplophyllum tuberculatum: An overview. Journal of Herbmed Pharmacology, 5(4), 125-130.
  18. Akhmedzhanova, V.I., Angenot, L., Shakirov, R.S. (2010). Alkaloids from Haplophyllum leptomerum. Chemistry of Natural Compounds, 46(3), 502-503. [CrossRef]
  19. Evcim, U., Gōzler, B., Freyer, A.J., Shamma, M. (1986). Haplomyrtin and (−)-haplomyrfolin: Two lignans from Haplophyllum myrtifolium. Phytochemistry, 25(8), 1949-1951. [CrossRef]
  20. Saglam, H., Gozler, T., Kivcak, B., Demirci, B., Baser, K.H.C. (2001). Composition of the essential oil of Haplophyllum myrtifolium. Chemistry of Natural Compounds, 37(5), 439-441. [CrossRef]
  21. Saglam, H., Gozler, T., Kivcak, B., Demirci, B., Baser, K.H.C. (2001). Volatile compounds from Haplophyllum myrtifolium. Chemistry of Natural Compounds, 37(5), 442-444. [CrossRef]
  22. Hamdi, A., Viane, J., Mahjoub, M.A., Majouli, K., Gad, M.H.H., Kharbach, M., Vander Heyden, Y. (2018). Polyphenolic contents, antioxidant activities and UPLC–ESI–MS analysis of Haplophyllum tuberculatum A. Juss leaves extracts. International Journal of Biological Macromolecules, 106, 1071-1079. [CrossRef]
  23. Zengin, G., Sarikurkcu, C., Aktumsek, A., Ceylan, R., Ceylan, O. (2014). A comprehensive study on phytochemical characterization of Haplophyllum myrtifolium Boiss. endemic to Turkey and its inhibitory potential against key enzymes involved in Alzheimer, skin diseases and type II diabetes. Industrial Crops and Products, 53, 244-251. [CrossRef]
  24. Gokmen, A.A., Can, H., Kayalar, H., Pektaş, B., Kaya, S. (2019). In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium. The Journal of Infection in Developing Countries, 13(03), 240-244. [CrossRef]
  25. Çetin, A., Erdoğan, N., Genç, H. (2012). Burdur gölü çevresinin tıbbi ve aromatik bitkilerine bir bakış. Tıbbi ve Aromatik Bitkiler Sempozyumu, 82-190, 13-15 September, Tokat, Turkiye.
  26. Massmanian, A., Prieto, J.M. (1996). Dermatitis de contacto por Haplophyllum hispanicum, 2a parte, perfil de una planta agresiva y curativa a la vez. 36 Reunıon del GEIDC, Grupo Espanol de Dermatitis de Contacto, 4 May, Sevilla, Spain.
  27. Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152-178. [CrossRef]
  28. Kumarasamy, Y., Byres, M., Cox, P.J., Jaspars, M., Nahar, L., Sarker, S.D. (2007). Screening seeds of some Scottish plants for free radical scavenging activity. Phytotherapy Research, 21(7), 615-621. [CrossRef]
  29. Re, R., Pellegrini, N., Proteggente, A., Pannalaa, A., Yanga, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorisation assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. [CrossRef]
  30. Barry, A.L. (1999). Methods for determining bactericidal activity of antimicrobial agents: Approved guideline, national committee for clinical laboratory standards publications, USA, pp.1-32.
  31. Humphries, R., Bobenchik, A.M., Hindler, J.A., Schuetz, A.N. (2021). Overview of changes to the clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing, M100. Journal of Clinical Microbiology, 59(12), 10-1128. [CrossRef]
  32. Yaman, C. (2023). Phytochemicals and antioxidant activities in methanol extracts of endemic Haplophyllum species from Türkiye. Sakarya University Journal of Science, 27(3), 687-696. [CrossRef]
  33. Plock, A., Sokolowska-Köhler, W., Presber, W. (2001). Application of flow cytometry and microscopical methods to characterize the effect of herbal drugs on Leishmania sp. Experimental Parasitology, 97(3), 141-153. [CrossRef]
  34. Ostan, I., Saglam, H., Limoncu, M.E., Ertabaklar, H., Toz, S.O., Ozbel, Y., Ozbilgin, A. (2007). In vitro and in vivo activities of Haplophyllum myrtifolium against Leishmania tropica. The New Microbiologica, 30(4), 439-445.
  35. Ashfaq, S., Hussain, M., Bibi, N., Alam, J., Junaid, M., Rehman, S.U. (2019). Antimicrobial and cytotoxic potential of Haplophyllum gilesii (Hemsl.) CC from Northern Pakistan. Pakistan Journal of Agricultural Research, 33(1), 146-153. [CrossRef]
  36. Al-Saeghi, S.S., Hossain, M.A., Al-Touby, S.S.J. (2022). Characterization of antioxidant and antibacterial compounds from aerial parts of Haplophyllum tuberculatum. Journal of Bioresources and Bioproducts, 7(1), 52-62. [CrossRef]
  37. Mitra, J.R., Eira, B.N., Bhawmick, A.K., Remon, M.K.I., Enan, M.E., Muhit, M.A., Zohora, F.T. (2022). Phytochemical and pharmacological aspects of genus Haplophyllum (Rutaceae): A review. International Journal of Health Sciences, 6(S9), 4454-4485.