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Journals / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 2025 / Cilt: 49 Sayı: 4-14th International Symposium on Pharmaceutical Sciences (ISOPS-14) Özel Sayı

FATTY ACID COMPOSITION AND BIOLOGICAL ACTIVITIES OF VITIS VINIFERA L. (ANTEP KARASI) SEED OIL

VITIS VINIFERA L. (ANTEP KARASI) YAĞININ YAĞ ASİDİ BİLEŞİMİ VE BİYOLOJİK AKTİVİTELERİ

Kübra Öğüt(ANADOLU ÜNİVERSİTESİ)0000-0002-9921-5866
Gülmira Özek(ANADOLU UNIVERSITY, FACULTY OF PHARMACY)0000-0001-8908-6098
Pervin Soyer(ANADOLU UNIVERSITY, FACULTY OF PHARMACY)0000-0002-6258-1993
Temel Özek(ANADOLU ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0003-4251-8783
Pages
1031–1043
Publication Date
2025-12-26
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Even at a concentration of 1875 µg/ml, biofilm inhibition of 50% and above was observed.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: This study examined the chemical composition and biological activity of Vitis vinifera L. (Antep Karası) seed oil, focusing on its fatty acid profile, phenolic content, and various antioxidant and antimicrobial properties.
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Key Findings & Data:Result and Discussion: V. vinifera (Antep Karası) seeds yielded 8.2% oil.
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Conclusion & Impact:Even at a concentration of 1875 µg/ml, biofilm inhibition of 50% and above was observed.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antep karası · biyolojik aktiviteler · GK-KS/AİD

Abstract

Objective: This study examined the chemical composition and biological activity of Vitis vinifera L. (Antep Karası) seed oil, focusing on its fatty acid profile, phenolic content, and various antioxidant and antimicrobial properties. Material and Method: Seeds of V. vinifera (known as “Antep Karası”) were collected from vines in Gaziantep, Türkiye, and the seed oil was extracted using n-hexane in a Soxhlet apparatus. Chemical composition of the oil was studied using GC-FID/MS after transesterification of fatty acids with BF₃ reagent. Standard spectrophotometric and microbiological tests were used to measure total flavonoids, phenolics, antioxidant capacity, enzyme inhibition, and antimicrobial, antifungal, and antibiofilm activities. Result and Discussion: V. vinifera (Antep Karası) seeds yielded 8.2% oil. Total phenolic and flavonoid content was 0.074 ± 0.001 mg GAE/gseed oil and 0.023 ± 0.001 mg RE/gseed oil. DPPH radical scavenging (IC₅₀ = 1.423 ± 0.004 mg/ml), β-carotene bleaching inhibition (0.182 ± 0.020 mg/ml), and TEAC value (0.012 µmol trolox/mgseed oil) were determined. The antibacterial evaluation revealed that the MBC values varied from 7500 -15000 µg/ml. Additionally, antifungal activity by MFC was observed with an MFC value of 7500 µg/ml. The antibiofilm assay revealed an inhibition activity in a dose-dependent manner. Even at a concentration of 1875 µg/ml, biofilm inhibition of 50% and above was observed.

Özet

Amaç: Bu çalışma, Vitis vinifera L. (Antep Karası) çekirdek yağının kimyasal bileşimini ve biyolojik aktivitesini incelemiş, özellikle yağ asidi profiline, fenolik içeriğine ve çeşitli antioksidan ve antimikrobiyal potansiyeline odaklanmıştır. Gereç ve Yöntem: Türkiye'nin Gaziantep ilindeki asmalardan toplanan V. vinifera (Antep Karası) çekirdeklerinden, n-hekzan kullanılarak Soxhlet cihazında yağ ekstraksiyonu gerçekleştirilmiştir. Yağ asitlerinin BF₃ reaktifi ile transesterifikasyonundan sonra, yağın kimyasal bileşimi GK-KS/AİD kullanılarak incelenmiştir. Toplam flavonoitler, fenolikler, antioksidan kapasite, enzim inhibisyonu ve antimikrobiyal, antifungal ve antibiyofilm aktivitelerini ölçmek için standart spektrofotometrik ve mikrobiyolojik testler kullanılmıştır. Sonuç ve Tartışma: V. vinifera (Antep Karası) çekirdeklerinden %8.2 oranında yağ elde edilmiştir. Toplam fenolik ve flavonoid miktarları sırasıyla 0.074 ± 0.001 mg GAE/gçekirdek yağı ve 0.023 ± 0.001 mg RE/gçekirdek yağı olarak belirlenmiştir. DPPH radikal süpürme (IC₅₀ = 1.423 ± 0.004 mg/ml), β-karoten ağarması inhibisyonu (0.182 ± 0.020 mg/ml) ve TEAC değeri (0.012 µmol trolox/mgçekirdek yağı) belirlenmiştir. Antibakteriyel değerlendirme, MBC değerlerinin 7500 - 15000 µg/ml aralığında değiştiği saptanmıştır. Ayrıca, MFC ile antifungal aktivite değeri 7500 µg/ml olarak belirlenmiştir. Antibiyofilm testi, doz bağımlı inhibisyon etkisi göstermiştir; 1875 µg/ml konsantrasyonda bile %50 ve üzeri biyofilm inhibisyonu gözlenmiştir.

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Cite This Article

Öğüt, K., Özek, G., Soyer, P., Özek, T. (2025). FATTY ACID COMPOSITION AND BIOLOGICAL ACTIVITIES OF VITIS VINIFERA L. (ANTEP KARASI) SEED OIL. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 49 Sayı: 4-14th International Symposium on Pharmaceutical Sciences (ISOPS-14) Özel Sayı, 1031–1043. https://doi.org/10.33483/jfpau.1773154

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