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Journals / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 2026 / Cilt: 50 Sayı: 1

EFFICIENT AND SENSITIVE ANALYSIS OF BIOMOLECULAR INTERACTIONS BETWEEN PALBOCICLIB AND HUMAN SERUM ALBUMIN USING FLUORESCENCE QUENCHING MECHANISM

PALBOSİKLİB VE İNSAN SERUM ALBÜMİN ARASINDAKI BİYOMOLEKÜLER ETKİLEŞİMLERİN FLORESAN SÖNÜMLEME MEKANİZMASI KULLANILARAK ETKİLİ VE HASSAS BİR ŞEKİLDE ANALİZ EDİLMESİ

Ahmet Cetinkaya(SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, GÜLHANE ECZACILIK FAKÜLTESİ, GÜLHANE ECZACILIK PR.)0000-0001-5014-0907
Merve Yence(LOKMAN HEKIM UNIVERSITY, FACULTY OF PHARMACY)0000-0003-4197-1009
Esen Bellur Atici(DEVA Holding A.S, R&D Center)0000-0002-4093-441X
Sibel A. Özkan(ANKARA ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0001-7494-3077
Pages
144–154
Publication Date
2026-01-14
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The van't Hoff equation was used to determine thermodynamic parameters such as entropy, Gibbs, and enthalpy at different temperatures.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: In this research, the interaction between Palbociclib (PC) and Human Serum Albumin (HSA) was examined using fluorescence spectroscopy, and the quenching mechanism was elucidated.
📊
Key Findings & Data:Material and Method: HSA and PC stock solutions were prepared using 0.1 M pH 7.4 phosphate buffer solution and DMSO, respectively.
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Conclusion & Impact:The van't Hoff equation was used to determine thermodynamic parameters such as entropy, Gibbs, and enthalpy at different temperatures.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Floresan spektroskopisi · ilaç-protein etkileşimi · insan serum albümini

Abstract

Objective: In this research, the interaction between Palbociclib (PC) and Human Serum Albumin (HSA) was examined using fluorescence spectroscopy, and the quenching mechanism was elucidated. Material and Method: HSA and PC stock solutions were prepared using 0.1 M pH 7.4 phosphate buffer solution and DMSO, respectively. In the protein-drug interaction experiment, the HSA concentration was fixed at 20 µM, and drug concentrations for the PC-HSA system were used between 0 and 40 µM. Result and Discussion: The interaction between PC and HSA was investigated at various temperatures using spectroscopic techniques. To comprehend the interaction process, experiments were carried out at different physiological pH levels and temperatures. Other parameters, including the number of binding sites, binding constants, and quenching constants, were calculated using the proper formulas. The van't Hoff equation was used to determine thermodynamic parameters such as entropy, Gibbs, and enthalpy at different temperatures.

Özet

Amaç: Bu çalışmada, Palbosiklib (PC) ile İnsan Serum Albümini (HSA) arasındaki etkileşim floresan spektroskopisi kullanılarak incelendi ve sönümleme mekanizması aydınlatıldı. Gereç ve Yöntem: HSA ve PC stok çözeltileri sırasıyla 0.1 M pH 7.4 fosfat tampon çözeltisi ve DMSO kullanılarak hazırlandı. Protein-ilaç etkileşimi deneyinde, HSA konsantrasyonu 20 µM'de sabitlendi ve PC-HSA sistemi için ilaç konsantrasyonları 0 ila 40 µM arasında kullanıldı. Sonuç ve Tartışma: PC ve HSA arasındaki etkileşim, spektroskopik teknikler kullanılarak çeşitli sıcaklıklarda incelenmiştir. Etkileşim sürecini anlamak için farklı fizyolojik pH seviyelerinde ve sıcaklıklarda deneyler gerçekleştirilmiştir. Bağlanma bölgesi sayısı, bağlanma sabitleri ve söndürme sabitleri gibi diğer parametreler, uygun formüller kullanılarak bulunmuştur. van't Hoff denklemi, farklı sıcaklıklarda entropi, Gibbs ve entalpi gibi termodinamik parametrelerini belirlemek için kullanıldı.

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Cite This Article

Cetinkaya, A., Yence, M., Atici, E. B., Özkan, S. A. (2026). EFFICIENT AND SENSITIVE ANALYSIS OF BIOMOLECULAR INTERACTIONS BETWEEN PALBOCICLIB AND HUMAN SERUM ALBUMIN USING FLUORESCENCE QUENCHING MECHANISM. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 50 Sayı: 1, 144–154. https://doi.org/10.33483/jfpau.1763144

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