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Journals / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 2026 / Cilt: 50 Sayı: 2

CHROMATOGRAPHIC AND COMPUTATIONAL APPROACHES TO THE PHARMACOKINETIC PROFILE OF PIROXICAM AND ITS SENSITIVE ASSAY IN RABBIT PLASMA

Tavşan plazmasında piroksikamın farmakokinetik profiline ve hassas analizine yönelik kromatografik ve hesaplamalı yaklaşımlar

Okan Ali Aksoy(UNIVERSITY OF HEALTH SCIENCES)0000-0003-4954-137X
Tugba Taskın Tok(FACULTY OF ARTS AND SCIENCES)0000-0002-0064-8400
Esra Merve Zanbak Çotaoğlu(UNIVERSITY OF HEALTH SCIENCES)0000-0002-6607-8973
Nurgul Karadas Bakirhan(SAĞLIK BİLİMLERİ ÜNİVERSİTESİ, GÜLHANE ECZACILIK FAKÜLTESİ, GÜLHANE ECZACILIK PR.)0000-0001-5469-9142
Engin Koçak(UNIVERSITY OF HEALTH SCIENCES)0000-0002-1076-1300
Berk Alp Göksel(UNIVERSITY OF HEALTH SCIENCES)0000-0002-1610-0618
Özgür Eşim(UNIVERSITY OF HEALTH SCIENCES)0000-0002-6402-156X
Ayhan Savaşer(UNIVERSITY OF HEALTH SCIENCES)0000-0002-2815-9254
Sibel A. Özkan(ANKARA UNIVERSITY)0000-0001-7494-3077
Yalçın Özkan(UNIVERSITY OF HEALTH SCIENCES, INSTITUTE OF HEALTH SCIENCES)0000-0001-5163-6867
Pages
350–361
Publication Date
2026-05-19
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The method showed linear performance over the concentration range of 0.1–4 mg/l.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: A simple, rapid, sensitive, and cost-effective high-performance liquid chromatographic (HPLC) method was developed for the pharmacokinetic study and determination of Piroxicam.
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Key Findings & Data:Material and Method: The chromatographic separation was performed with a Agilent ZORBAX reversed-phase C18 column (250 mm × 4,6 mm ID × 5 μm).
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Conclusion & Impact:The method showed linear performance over the concentration range of 0.1–4 mg/l.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:ADMET · DFT hesaplaması · farmakokinetik analiz

Abstract

Objective: A simple, rapid, sensitive, and cost-effective high-performance liquid chromatographic (HPLC) method was developed for the pharmacokinetic study and determination of Piroxicam. Material and Method: The chromatographic separation was performed with a Agilent ZORBAX reversed-phase C18 column (250 mm × 4,6 mm ID × 5 μm). Mobile phase composition was 0.04 M KH 2 PO 4 : acetonitrile:methanol (50:40:10) at pH 3.8. The flow rate was adjusted as 0.9 mL/min. Result and Discussion: An isocratic method was used for the analysis. The UV detector was set at 360 nm, and a retention time of 7 minutes was obtained for piroxicam. The method showed linear performance over the concentration range of 0.1–4 mg/l.

Özet

Amaç: Piroksikam’ın farmakokinetik çalışması ve tayini için basit, hızlı, hassas ve maliyet etkin bir yüksek performanslı sıvı kromatografi (HPLC) yöntemi geliştirilmiştir. Gereç ve Yöntem: Kromatografik ayrım, Agilent ZORBAX ters fazlı C18 kolon (250 mm × 4,6 mm ID × 5 μm) kullanılarak gerçekleştirilmiştir. Mobil faz bileşimi, pH 3.8’de 0.04 M KH₂PO₄:asetonitril:metanol (50:40:10) oranında hazırlanmıştır. Akış hızı 0.9 ml/dk olarak ayarlanmıştır. Sonuç ve Tartışma: Analiz için izokratik yöntem kullanılmıştır. UV dedektörü 360 nm’ye ayarlanmış ve piroksikam için 7 dakikalık bir alıkonma süresi elde edilmiştir. Yöntem, 0.1–4 mg/l konsantrasyon aralığında doğrusal performans göstermiştir.

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Cite This Article

Aksoy, O. A., Tok, T. T., Çotaoğlu, E. M. Z., Bakirhan, N. K., Koçak, E., Göksel, B. A., Eşim, Ö., Savaşer, A., Özkan, S. A., Özkan, Y. (2026). CHROMATOGRAPHIC AND COMPUTATIONAL APPROACHES TO THE PHARMACOKINETIC PROFILE OF PIROXICAM AND ITS SENSITIVE ASSAY IN RABBIT PLASMA. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 50 Sayı: 2, 350–361. https://doi.org/10.33483/jfpau.1766558

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