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Journals / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 2026 / Cilt: 50 Sayı: 3

INVESTIGATION OF THE ANTIMICROBIAL AND ANTICANCER EFFECT OF THE PLANTS CONSOLIDA ORIENTALIS (GAY) SCHROD., AESCULUS HIPPOCASTANUM L., AND SYRINGA VULGARIS L.

CONSOLIDA ORIENTALIS (GAY) SCHROD., AESCULUS HIPPOCASTANUM L. VE SYRINGA VULGARIS L. BİTKİLERİNİN ANTİMIKROBİYAL VE ANTİKANSER ETKİLERİNİN ARAŞTIRILMASI

Gonca Şimşek(SİVAS CUMHURİYET ÜNİVERSİTESİ)0000-0001-6165-1614
Zeynep Sümer(SIVAS CUMHURIYET UNIVERSITY, SCHOOL OF MEDICINE)0000-0002-1520-3359
Tutku Tunç(SIVAS CUMHURIYET UNIVERSITY, FACULTY OF PHARMACY)0000-0002-8274-9386
Hülya Özpınar(SIVAS CUMHURIYET UNIVERSITY, FACULTY OF PHARMACY)0000-0001-8154-0874
Pages
693–704
Publication Date
2026-09-19
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The lowest MIC value was observed in S. vulgaris extract, and the lowest IC 50 value in A. hippocastanum extract.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: This study was designed to investigate the anticancer effects of ethanolic extracts of Consolida orientalis, Aesculus hippocastanum, and Syringa vulgaris on the C6 glioma cell line and the antimicrobial effect on pathogen microorganisms.
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Key Findings & Data:Anticancer activity was assessed via XTT assay for C6-glioma and HT-22 cell lines.
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Conclusion & Impact:The lowest MIC value was observed in S. vulgaris extract, and the lowest IC 50 value in A. hippocastanum extract.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Aesculus hippocastanum · antikanser · antimikrobiyal

Abstract

Objective: This study was designed to investigate the anticancer effects of ethanolic extracts of Consolida orientalis, Aesculus hippocastanum, and Syringa vulgaris on the C6 glioma cell line and the antimicrobial effect on pathogen microorganisms. Material and Method: Strains used in the study were obtained from the Medical Microbiology Laboratory of Sivas Cumhuriyet University Hospital. Plant extracts were evaluated using microdilution broth method for antimicrobial activity. Anticancer activity was assessed via XTT assay for C6-glioma and HT-22 cell lines. Chemical composition of plant extracts characterized using GC-MS analysis. Result and Discussion: The MIC values of the microbial strains ranged from 2.5 to >20 mg/ml, according to the findings. The extracts showed no activity against Candida albicans and Pseudomonas aeruginosa at the 20 mg/ml. The effective doses of the plant extracts on the C6 glioma were calculated using the IC₅₀ method, values in the range of 186–500 μg/ml. The cytotoxic effect of the extracts on the HT-22 healthy cell line was found to exceed 500 µg/ml in extracts other than C. orientalis. The lowest MIC value was observed in S. vulgaris extract, and the lowest IC 50 value in A. hippocastanum extract.

Özet

Amaç: Bu çalışma, Consolida orientalis, Aesculus hippocastanum ve Syringa vulgaris'in etanolik ekstrelerinin C6 glioma hücre hattı üzerindeki antikanser etkisini ve patojen mikroorganizmalar üzerindeki antimikrobiyal etkisini araştırmak amacıyla tasarlanmıştır. Gereç ve Yöntem: Çalışmada kullanılan suşlar Sivas Cumhuriyet Üniversitesi Hastanesi Tıbbi Mikrobiyoloji Laboratuvarı'ndan temin edilmiştir. Bitki ekstrelerinin antimikrobiyal aktiviteleri mikrodilüsyon broth metodu kulanılarak araştırılmıştır. C6-glioma hücre hattı üzerinde antikanser aktivite XTT yöntemi ile değerlendirilmiştir. Bitki ekstrelerinin kimyasal bileşimi GC-MS analizi kullanılarak karakterize edilmiştir. Sonuç ve Tartışma : Bulgulara göre, mikrobiyal suşların MIC değerleri 2.5 ile >20 mg/ml arasında değişmektedir. Ekstreler 20 mg/ml’de Candida albicans ve Pseudomonas aeruginosa’ya karşı herhangi bir aktivite göstermemiştir. Bitki ekstrelerinin C6 glioma hücre hattı üzerindeki etkili dozları, IC₅₀ yöntemi kullanılarak hesaplanmış, değerler 186–500 μg/ml aralığında tespit edilmiştir. Ekstrelerin HT-22 sağlıklı hücre hattı üzerindeki sitotoksik etkisinin, C. orientalis dışındaki ekstrelerde 500 µg/ml'yi aştığı tespit edilmiştir. En düşük MİK değeri S. vulgaris ekstresinde, en düşük IC 50 değeri ise A. hippocastanum ekstresinde gözlemlenmiştir.

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Cite This Article

Şimşek, G., Sümer, Z., Tunç, T., Özpınar, H. (2026). INVESTIGATION OF THE ANTIMICROBIAL AND ANTICANCER EFFECT OF THE PLANTS CONSOLIDA ORIENTALIS (GAY) SCHROD., AESCULUS HIPPOCASTANUM L., AND SYRINGA VULGARIS L.. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 50 Sayı: 3, 693–704. https://doi.org/10.33483/jfpau.1782946

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References (45)

  1. Rocchetti, G., Zengin, G., Cakmak, Y.S., et al. (2020). A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey. Industrial Crops and Products, 158, 112966. [CrossRef]
  2. Yin, T., Cai, L., Ding, Z. (2020). A systematic review on the chemical constituents of the genus Consolida (Ranunculaceae) and their biological activities. RSC Advances, 10(58), 35072-35089. [CrossRef]
  3. Ozpinar, H. (2021). Anti-helminthic activity of Consolida orientalis (Gay) Schröd. on Caenorhabditis elegans nematodes and determination of possible active ingredients. Turkish Journal of Agriculture-Food Science and Technology, 9(8), 1593-1598. [CrossRef]
  4. Demirezer, Ö., Ersöz, T., Saraçoğlu, İ., Şener, B. (2011). FFD Monografları Tedavide Kullanılan Bitkiler, MN Medikal and Nobel Tıp Kitapevi, Ankara, 9-12.
  5. Mladenova, R., Solakov, N., Loginovska, K., et al. (2025). Analysis of gamma-irradiation effect on radicals formation and on antiradical capacity of horse chestnut (Aesculus hippocastanum l.) seeds. Applied Sciences, 15(6), 3287. [CrossRef]
  6. Idris, S., Mishra, A., Khushtar, M. (2020). Phytochemical, ethanomedicinal and pharmacological applications of escin from Aesculus hippocastanum L. towards future medicine. Journal of Basic and Clinical Physiology and Pharmacology, 31(5), 20190115. [CrossRef]
  7. Yan, L., Zhang, Y., Dai, D., et al. (2025). Pharmacological activities of Aesculus hippocastanum and its bioactive constituents in hemostasis: A mechanistic review. Pharmacognosy Magazine, 21(3), 727-737. [CrossRef]
  8. Khar’kov, Y.K., Arsene, M.M., Aliya, M.V., et al. (2022). Assessment of antimicrobial activity of ethanolic and aqueous extracts of Aesculus hippocastanum L. (Horse Chestnut) bark against bacteria isolated from urine of patients diagnosed positive to urinary tract infections. Frontiers in Bioscience-Scholar, 14(2), 11. [CrossRef]
  9. Mortazavi-Derazkola, S., Samadipour, M., Mohammadparast-Tabas, P., et al. (2025). Aesculus hippocastanum extract-mediated biosynthesis of silver-decorated zinc oxide nanoparticles and investigation of their photocatalytic, antibacterial, and antioxidant properties. Bioprocess and Biosystems Engineering, 1-14. [CrossRef]
  10. Mihai, D.P., Seremet, O.C., Nitulescu, G., et al. (2019). Evaluation of natural extracts in animal models of pain and inflammation for a potential therapy of hemorrhoidal disease. Scientia Pharmaceutica, 87(2), 14. [CrossRef]
  11. Wang, Q.H., Huo, S.R.N., Bao, Y.P., et al. (2018). Chemical constituents of Syringa pinnatifolia and its chemotaxonomic study. Chemistry of Natural Compounds, 54(3), 435-438. [CrossRef]
  12. Zhu, W., Wang, Z., Sun, Y., et al. (2021). Traditional uses, phytochemistry and pharmacology of genus Syringa: A comprehensive review. Journal of Ethnopharmacology, 266, 113465. [CrossRef]
  13. Lewandowska, N., Klimowicz, A. (2022). Antioxidant properties of selected parts of Syringa vulgaris L. Pomeranian Journal of Life Sciences, 68(3). [CrossRef]
  14. Zhang, M., Zhai, X., He, L., et al. (2025). Morphological description and DNA barcoding research of nine Syringa species. Frontiers in Genetics, 16, 1544062. [CrossRef]
  15. Abd-elmegeed, A.S.S., Saad Abd-alrahman, H., Mohamed, A.A., et al. (2024). Medicinal plants: Past traditions and hope of tomorrow. International Journal of Science and Research Archive, 2024, 13(02), 3789-3797. [CrossRef]
  16. Atanasov, A.G., Waltenberger, B., Pferschy-Wenzig, E.M., et al. (2015). Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnology Advances, 33(8), 1582-1614. [CrossRef]
  17. Waheed, M., Hussain, M.B., Saeed, F., et al. (2024). Phytochemical profiling and therapeutic potential of thyme (Thymus spp.): A medicinal herb. Food Science & Nutrition, 12(12), 9893-9912. [CrossRef]
  18. Döşler, S., Özdemir, R., Yılmaz, F. (2019). Bazı bitki ekstrelerinin antimikrobiyal etkilerinin araştırılması. Türk Farmakope Dergisi, 4(3), 17-28.
  19. Chaughule, R.S. and Barve, R.S. (2024). Role of herbal medicines in the treatment of infectious diseases. Vegetos, 37(1), 41-51. [CrossRef]
  20. Bray, F., Laversanne, M., Sung, H., et al. (2024). Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 74(3), 229-263. [CrossRef]
  21. Talib, W.H., Alsalahat, I., Daoud, S., et al. (2020). Plant-derived natural products in cancer research: extraction, mechanism of action, and drug formulation. Molecules, 25(22), 5319. [CrossRef]
  22. Bray, F., Ferlay, J., Soerjomataram, I., et al. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68(6), 394-424. [CrossRef]
  23. Rayan, A., Raiyn, J., Falah, M. (2017). Nature is the best source of anticancer drugs: Indexing natural products for their anticancer bioactivity. PloS One, 12(11), e0187925. [CrossRef]
  24. Giakoumettis, D., Kritis, A., Foroglou, N. (2018). C6 cell line: The gold standard in glioma research. Hippokratia, 22(3), 105.
  25. Weller, M., Wen, P.Y., Chang, S.M., et al. (2024). Glioma. Nature Reviews Disease Primers, 10(1), 33. [CrossRef]
  26. EUCAST The European committee on antimicrobial susceptibility testing website. (2025). https://www.eucast.org/bacteria/clinical-breakpoints-and-interpretation/clinical-breakpoint-tables. Accessed date: 14.08.2025.
  27. EUCAST The European committee on antimicrobial susceptibility testing website. (2025). https://www.eucast.org/fungi-afst/clinical-breakpoints-and-interpretation/clinical-breakpoint-tables. Accessed date: 14.08.2025.
  28. Holetz, F.B., Pessini, G.L., Sanches, N.R., et al. (2002). Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Memórias do Instituto Oswaldo Cruz, 97, 1027-1031. [CrossRef]
  29. Jimenez‐Arellanes, A., Meckes, M., Ramirez, R., et al. (2003). Activity against multidrug‐resistant Mycobacterium tuberculosis in Mexican plants used to treat respiratory diseases. Phytotherapy Research, 17(8), 903-908. [CrossRef]
  30. Kuete, V. (2010). Potential of Cameroonian plants and derived products against microbial infections: A review. Planta Medica, 76(14), 1479-1491. [CrossRef]
  31. Awouafack, M.D., Tane, P., Kuete, V., Eloff, J.N. (2013). Sesquiterpenes from the Medicinal Plants of Africa. Ed: Kuete V., In Medicinal Plant Research in Africa, Waltham, MA, 33-103.
  32. Eruygur, N., Uçar, E., Tüzün, B., et al. (2024). Evaluation of antioxidant, antimicrobial, enzyme inhibition activity, and cell viability capacity of Hypericum heterophyllum vent., an endemic species in Turkey’s Flora. Journal of Molecular Structure, 1307, 137908. [CrossRef]
  33. El-Shiekh, R.A., Meselhy, M.R., Elshimy, R., et al. (2024). Plumieride as a novel anti-fungal and anti-inflammatory iridoid against superficial candidiasis in mice. BMC Complementary Medicine and Therapies, 24(1), 224. [CrossRef]
  34. Tunç, T. (2024). Synthesis and characterization of silver nanoparticles loaded with carboplatin as a potential antimicrobial and cancer therapy. Cancer Nanotechnology, 15(1), 2. [CrossRef]
  35. Tunç, T., Ozpinar, H., Koçyiğit, Ü.M., et al. (2024). Exploring the antioxidant, antimicrobial, antidiabetic, and anticancer properties of Capsicum annuum L. (Samandağ Pepper): Biochemical and in silico insights. ChemistrySelect, 9(48), e202403760. [CrossRef]
  36. Maregesi, S.M., Pieters, L., Ngassapa, O.D., et al. (2008). Screening of some Tanzanian medicinal plants from Bunda district for antibacterial, antifungal and antiviral activities. Journal of Ethnopharmacology, 119(1), 58-66. [CrossRef]
  37. Nadaf, N.H., Parulekar, R.S., Patil, R.S., et al. (2018). Biofilm inhibition mechanism from extract of Hymenocallis littoralis leaves. Journal of Ethnopharmacology, 222, 121-132. [CrossRef]
  38. Şimşek, G., Poyraz, Ö. (2025). Investigation of the antimicrobial and antibiofilm effect of plant Consolida orientalis on methicillin resistant Staphylococcus aureus (MRSA) and vancomycin resistant Enterococcus sp.(VRE). International Journal of Secondary Metabolite, 12(2), 343-354. [CrossRef]
  39. Mohammed, M.J., Younus, A. (2020). Chemical characteristics and antimicrobial effect of essential oil and fatty acids isolated from Foeniculum vulgare seeds. International Journal of Psychosocial Rehabilitation, 24(05). [CrossRef]
  40. Al-Abdulsalam, H., Almalki, M., Khalifa, A. (2025). Isolation and characterization of antimicrobial metabolites producing bacteria from soils in Al-Ahsa, Saudi Arabia. Journal of Microbiology, Biotechnology and Food Sciences, 14(5), e11779-e11779. [CrossRef]
  41. Dridi, A., Reis, F.S., Pires, T.C., et al. (2023). Aesculus hippocastanum L.: A simple ornamental plant or a source of compelling molecules for industry? Separations, 10(3), 160. [CrossRef]
  42. Alshehri, A., Ahmad, A., Tiwari, R.K., et al. (2022). In vitro evaluation of antioxidant, anticancer, and anti-inflammatory activities of ethanolic leaf extract of Adenium obesum. Frontiers in Pharmacology, 13, 847534. [CrossRef]
  43. Isbilen, O., Volkan, E. (2021). Allium willeanum Holmboe exerts anticancer activities on metastatic breast cancer cells MCF-7 and MDA-MB-231. Heliyon, 7(8). [CrossRef]
  44. Ghosh, S., Sinha, D. (2025). Indian perspective of PM2.5 attributed human health hazards during 2010-2025. Air Quality, Atmosphere and Health, 1-40. [CrossRef]
  45. Roshni, A.S., Elizabeth, A.A., Aishwarya, S.S. (2022). In vitro cytotoxic action of Aesculus hippocastanum extract against PA 1 human ovarian cell-line. Biomedical and Pharmacology Journal, 15(3), 1445-1451. [CrossRef]