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Journals / European Journal of Biology / 2026 / Volume: 81 Issue: 1

Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods

Beliz Yüksektepe(İstanbul University, Institute of Sciences, Programme of Molecular Biology and Genetics, Istanbul, Turkiye)0000-0001-7703-2981
Özlem Sefer(İstanbul Yeni Yüzyıl University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkiye)0000-0002-2711-5938
Gülin İnci Varol(Istanbul University, Faculty of Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkiye)0000-0003-0091-1245
Tuğba Teker(stanbul University, Institute of Sciences, Programme of Molecular Biotechnology and Genetics, Istanbul, Turkiye)0000-0001-9892-8429
Mehmet Arslan(İstanbul University, Institute of Sciences, Programme of Molecular Biotechnology and Genetics, Istanbul, Turkiye)0000-0003-0613-8978
Büşra Nur Çetin(İstanbul University, Institute of Sciences, Programme of Molecular Biotechnology and Genetics, Istanbul, Turkiye)0000-0002-3917-867X
Figen Mert(Canakkale Onsekiz Mart University, Faculty of Agriculture, Department of Plant Protection, Canakkale, Turkiye)0000-0002-1603-0226
Emre Yörük(İstanbul Yeni Yüzyıl University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkiye)0000-0003-2770-0157
Gülruh Albayrak(Istanbul University, Faculty of Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkiye)0000-0002-4499-8912
Pages
—
Publication Date
2022-06-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our studies will contribute to disease profiling and it is the first step in disease management.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Fusarium spp. cause Fusarium head blight (FHB) and crown rot (CR) diseases.
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Key Findings & Data:Results: Thirty-eight F. graminearum and 30 F. culmorum isolates were identified via amplification of UBC85 and OPT18 SCAR markers, respectively.
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Conclusion & Impact:Our studies will contribute to disease profiling and it is the first step in disease management.
🔬Design:Short Communication
📍Scope:2010 Verileri
⚙️Variables:Fusarium spp. · macroconidia · SCAR marker

Abstract

Objective: Fusarium spp. cause Fusarium head blight (FHB) and crown rot (CR) diseases. They also have harmful effects on animal and human health through their mycotoxins. Within the scope of this study, F. graminearum and F. culmorum isolates were purified from wheat ears and stalks contaminated with phytopathogens, which had been collected from various regions of Turkey, were identified and characterized by conventional and molecular methods. Materials and Methods: Sixty-eight Fusarium samples were isolated by single spore analysis and classified according to their macroconidia shape and size. Morphologically characterized samples were verified by amplification of SCAR markers. Their mating types (MAT) and chemotypes were also determined through polymerase chain reaction (PCR). Results: Thirty-eight F. graminearum and 30 F. culmorum isolates were identified via amplification of UBC85 and OPT18 SCAR markers, respectively. All isolates were determined as trichothecene producers by amplification of the tri5 gene. All F. graminarum isolates carry both MAT-1 and MAT-2 loci, whereas 7 of F. culmorum isolates were also determined as MAT-1 and 23 of them as MAT-2 mating types. Deoxynivalenol production capacity of all isolates was identified by tri13 amplification for chemotype determination. Conclusion: Routine monitoring of phytopathogens and their mycotoxin levels is a requirement since their annual levels may vary depending on environmental factors. This work provides knowledge about the distribution of Fusarium spp. leading to FHB and CR in different regions of Turkey between 2010 and 2020. Also, their chemotypes were demonstrated. Our studies will contribute to disease profiling and it is the first step in disease management.

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Cite This Article

Yüksektepe, B., Sefer, Ö., Varol, G. İ., Teker, T., Arslan, M., Çetin, B. N., Mert, F., Yörük, E., Albayrak, G. (2022). Identification of Fusarium graminearum and Fusarium culmorum Isolates via Conventional and Molecular Methods. European Journal of Biology, Volume: 81 Issue: 1. https://doi.org/10.26650/EurJBiol.2022.1078448

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