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Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 8 Sayı: 2

Investigation of the Effects of Eugenol on Fusarium culmorum

Eugenol’ün Fusarium culmorum Üzerindeki Etkilerinin İncelenmesi

Emre Yörük0000-0003-2770-0157
Özlem Sefer0000-0002-2711-5938
Ayşe Server Sezer0000-0001-6869-8757
Zeynep Konukcu0000-0002-7854-3131
Elif Sedef Develi0000-0001-6696-7001
Pages
215–221
Publication Date
2018-06-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Normalization results showed that fold changes in FcMgv1 and FcStuA genes were as +4.35±0.25 and +2.04±0.13, respectively. qPCR results were also confirmed via reverse transcription PCR (RT-PCR) analysis.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Fusarium culmorum is one of the causal agents lead to economic loses in small grain cereals.
📊
Key Findings & Data:In this study, alterations in spore production, and linear growth rates in F. culmorum 15 isolate subjected to increased concentrations of eugenol (0, 200, 400, 800 µg mL-1) were examined.
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Conclusion & Impact:Normalization results showed that fold changes in FcMgv1 and FcStuA genes were as +4.35±0.25 and +2.04±0.13, respectively. qPCR results were also confirmed via reverse transcription PCR (RT-PCR) analysis.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antifungal · Doğrusal büyüme oranı · FcStua

Abstract

Fusarium culmorum is one of the causal agents lead to economic loses in small grain cereals. In this study, alterations in spore production, and linear growth rates in F. culmorum 15 isolate subjected to increased concentrations of eugenol (0, 200, 400, 800 µg mL-1) were examined. Additionally, the and expression of FcMgv1 and FcStuA genes which are essential in asexual stage and cell wall structure formation were also examined tested in F. culmorum eugenol treated and non-treated fungal cultures. F15 isolate subjected to increased concentrations of eugenol (0, 200, 400, 800 µg mL-1). Minimum inhibitory concentration was determined as 400 µg mL-1 eugenol. In comparison of control and experiment sets, there were significantly different (p<0.01) the decrease in spore production and linear growth rate (LGR) were significantly different (p<0.01). In real time polymerase chain reaction (qPCR) analysis, β-tubulin was used as endogenous control and the expression of FcMgv1 and FcStuA genes were determined by using cDNAs converted from total RNAs of control and experiment sets were used in Eva-Green fluorophore dyebased real time PCR to examine the FcMgv1 and FcStuA expression. Significant differences (p<0.05) were also determined in fold changes in gene expression. Normalization results showed that fold changes in FcMgv1 and FcStuA genes were as +4.35±0.25 and +2.04±0.13, respectively. qPCR results were also confirmed via reverse transcription PCR (RT-PCR) analysis. The late apoptosis was detected in the cells by using acridine orange/ethidium bromide staining. Findings obtained from this study showed that eugenol have the potential antifungal effects and it could be used in struggling with head blight disease caused by F. culmorum via its in vitro antifungal effects. This study presents the original data on about is first report to evaluatinge the phenotypic and transcriptomic effects of pure eugenol compound on F. culmorum.

Özet

Fusarium culmorum küçük daneli tahıllarda ekonomik kayıplara yol açan hastalık etmenlerinden birisidir.

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Cite This Article

Yörük, E., Sefer, Ö., Sezer, A. S., Konukcu, Z., Develi, E. S. (2018). Investigation of the Effects of Eugenol on Fusarium culmorum. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 8 Sayı: 2, 215–221. https://doi.org/10.21597/jist.428382

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